Rabbit Recombinant Monoclonal Ergocalciferol antibody. Carrier free. Suitable for cELISA and reacts with Chemical samples.
Images
![Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (AB236471), expandable thumbnail](https://content.abcam.com/products/images/vitamin-d2vitamin-d3-antibody-epptx-r6-74-3-bsa-and-azide-free-ab236471--competitive-elisa-img63638.jpg/_jcr_content/renditions/webp-475.webp "Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (AB236471)")
![Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (AB236471), expandable thumbnail](https://content.abcam.com/products/images/vitamin-d2vitamin-d3-antibody-epptx-r6-74-3-bsa-and-azide-free-ab236471--competitive-elisa-img39383.jpg/_jcr_content/renditions/webp-475.webp "Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (AB236471)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| cELISA | |
|---|---|
| Chemical | Tested |
cELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes Use at 30-35 ng/mL. |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Additional Targets
Vitamin D3
Alternative names
Ergocalciferol
Recommended products
Rabbit Recombinant Monoclonal Ergocalciferol antibody. Carrier free. Suitable for cELISA and reacts with Chemical samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPPTX-R6-74-3
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab236471 is the carrier-free version of Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] ab229821.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (ab236471), expandable thumbnail](https://content.abcam.com/products/images/vitamin-d2vitamin-d3-antibody-epptx-r6-74-3-bsa-and-azide-free-ab236471--competitive-elisa-img63638.jpg "Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (ab236471)")
Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (ab236471)
Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] (Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] ab229821).Goat anti-rabbit IgG was coated onto 96-wells. Serial dilutions (0, 2.5, 5, 10, 25, 50, 100 ng/mL) of 25-OH-Vitamin-D (25 μL) were incubated with 30-35 ng/mL of Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] ab229821 (50 μL) for 30 minutes by shaking.
This was followed by adding HRP-conjugated 25-OH-Vitamin-D (50 μL) into each well and incubating for another 30 minutes. After washing, 125 μL Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. OD was read at 450nm within 5 minutes.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
![Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (ab236471), expandable thumbnail](https://content.abcam.com/products/images/vitamin-d2vitamin-d3-antibody-epptx-r6-74-3-bsa-and-azide-free-ab236471--competitive-elisa-img39383.jpg "Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (ab236471)")
Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (ab236471)
Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] (Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] ab229821) used at 8.3 ng/mL.The results show cross-reactivity with Vitamin D2.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
Downloads
Product protocols
- Visit the general protocols
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