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Rabbit Recombinant Monoclonal Vitamin D3 antibody. Suitable for cELISA and reacts with Chemical samples.

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Images

Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (AB229820), expandable thumbnail
  • Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (AB229820), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
cELISA
Chemical
Tested

Tested
Tested

Species
Chemical
Dilution info
0.03-0.035 µg/mL
Notes

-

Target data

Alternative names

cholecalciferol

Recommended products

Rabbit Recombinant Monoclonal Vitamin D3 antibody. Suitable for cELISA and reacts with Chemical samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPPTX-R6-42-12
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820), expandable thumbnail

    Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820)

    Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820)

    Goat anti-rabbit IgG was coated onto 96-wells. Serial dilutions (0, 2.5, 5, 10, 25, 50, 100 ng/mL) of 25-OH-Vitamin-D (25 μL) were incubated with 20-25 ng/mL of ab229820 (50 μL) for 30 minutes by shaking.

    This was followed by adding HRP-conjugated 25-OH-Vitamin-D (50 μL) into each well and incubating for another 30 minutes. After washing, 125 μL of Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. OD was read at 450nm within 5 minutes.

    This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

  • Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820), expandable thumbnail

    Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820)

    Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820) used at 6.3 ng/ml.

    This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com