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AB236470

Anti-Vitamin D3 antibody [EPPTX-R6-42-12] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Vitamin D3 antibody. Carrier free. Suitable for cELISA and reacts with Chemical samples. Cited in 1 publication.

View Alternative Names

cholecalciferol

2 Images
Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] - BSA and Azide free (AB236470)
  • cELISA

Supplier Data

Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] - BSA and Azide free (AB236470)

Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820) used at 6.3 ng/ml.

This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] - BSA and Azide free (AB236470)
  • cELISA

Supplier Data

Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] - BSA and Azide free (AB236470)

Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820)

Goat anti-rabbit IgG was coated onto 96-wells. Serial dilutions (0, 2.5, 5, 10, 25, 50, 100 ng/mL) of 25-OH-Vitamin-D (25 μL) were incubated with 20-25 ng/mL of ab229820 (50 μL) for 30 minutes by shaking.

This was followed by adding HRP-conjugated 25-OH-Vitamin-D (50 μL) into each well and incubating for another 30 minutes. After washing, 125 μL of Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. OD was read at 450nm within 5 minutes.

This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPPTX-R6-42-12

Isotype

IgG

Carrier free

Yes

Applications

cELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "cELISA-species-checked": "testedAndGuaranteed", "cELISA-species-dilution-info": "", "cELISA-species-notes": "<p>Use at 30-35 ng/mL.</p>" } } }
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Product details

ab236470 is the carrier-free version of ab229820.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Vitamin D3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Genes & genomics 47:961-972 PubMed40794264

2025

NCOA4 inhibits glioma progression by suppressing the Sonic Hedgehog pathway and its overexpression indicates a better glioma prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Kaining Liu,Hu Wang,Tian Qiu,Guangxiu Wang,Anling Zhang,Zhifan Jia,Xiaoguang Tong
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com