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AB235987

Anti-Vitronectin/S-Protein antibody

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(1 Publication)

Rabbit Polyclonal Vitronectin/S-Protein antibody. Suitable for WB, IP, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Vitronectin aa 350 to C-terminus.

View Alternative Names

Vitronectin, VN, S-protein, Serum-spreading factor, V75, VTN

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody (AB235987)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody (AB235987)

Paraffin-embedded human placenta tissue stained for Vitronectin/S-Protein using ab235987 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody (AB235987)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody (AB235987)

Paraffin-embedded human small intestine tissue stained for Vitronectin/S-Protein using ab235987 at 1/100 dilution in immunohistochemical analysis.

Immunoprecipitation - Anti-Vitronectin/S-Protein antibody (AB235987)
  • IP

Supplier Data

Immunoprecipitation - Anti-Vitronectin/S-Protein antibody (AB235987)

Vitronectin/S-Protein was immunoprecipitated from 500 μg NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate with ab235987.

Lane 1 : Rabbit control IgG IP in NIH/3T3 whole cell lysate.

Lane 2 : ab235987 IP in NIH/3T3 whole cell lysate.

Lane 3 : NIH/3T3 whole cell lysate 10 μg (Input).

For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.

All lanes:

Immunoprecipitation - Anti-Vitronectin/S-Protein antibody (ab235987)

Predicted band size: 54 kDa,62 kDa

false

Western blot - Anti-Vitronectin/S-Protein antibody (AB235987)
  • WB

Supplier Data

Western blot - Anti-Vitronectin/S-Protein antibody (AB235987)

All lanes:

Western blot - Anti-Vitronectin/S-Protein antibody (ab235987) at 12 µg/mL

All lanes:

HepG2 whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Observed band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human Vitronectin aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P04004

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "11 µg/mL", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.. Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
See full target information Vitronectin
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurobiology 61:1936-1952 PubMed37819429

2023

Teriflunomide Promotes Blood-Brain Barrier Integrity by Upregulating Claudin-1 via the Wnt/β-catenin Signaling Pathway in Multiple Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yipeng Zhao,Chen Chen,Xiuqing Xiao,Ling Fang,Xi Cheng,Yanyu Chang,Fuhua Peng,Jingqi Wang,Shishi Shen,Shilin Wu,Yiying Huang,Wei Cai,Linli Zhou,Wei Qiu
View all publications
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Product promise

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