Rabbit Recombinant Monoclonal Vitronectin/S-Protein antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 4 publications.
Images
![Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808), expandable thumbnail](https://content.abcam.com/products/images/vitronectin-s-protein-antibody-ep781y-ab46808--western-blot-img32188.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)")
![Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808), expandable thumbnail](https://content.abcam.com/products/images/vitronectin-s-protein-antibody-ep781y-ab46808--immunoprecipitation-img6105.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808), expandable thumbnail](https://content.abcam.com/products/images/vitronectin-s-protein-antibody-ep781y-ab46808--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73787.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)")
Publications
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- Endocrine journal 68:543-5522021Circular RNA circ_0008274 enhances the malignant progression of papillary thyroid carcinoma via modulating solute carrier family 7 member 11 by sponging miR-154-3p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingjing Ma,Zhenghua KanPubMed 33473055
- Ultrasound in medicine & biology 45:3102-31152019Mechanical Weakness of Thoracic Aorta Related to Aging or Dissection Predicted by Speed of Sound with Collagenase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatsutoshi Miura et. al.PubMed 31537389
- International journal of clinical and experimental 8:11364-712015Vitronectin significantly influences prognosis in osteosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Shi et. al.PubMed 26617861
- Molecular medicine reports 7:53-82012Increased expression levels of vitronectin in the maternal‑fetal interface of placenta in early-onset severe preeclampsia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Shen,Xiaoyan Ma,Fei Yi,Azhar Rasul,Manhua Cui,Tonghui MaPubMed 23124223
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Associated Products
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Target data
Function
Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
Alternative names
Vitronectin, VN, S-protein, Serum-spreading factor, V75, VTN
Recommended products
Rabbit Recombinant Monoclonal Vitronectin/S-Protein antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP781Y
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
This antibody has not been purified. It is provided in tissue culture supernatant and storage buffer.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808), expandable thumbnail](https://content.abcam.com/products/images/vitronectin-s-protein-antibody-ep781y-ab46808--western-blot-img32188.jpg "Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)")
Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)
All lanes: Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808) at 1/20000 dilution
All lanes: Human serum at 10 µg
SecondaryAll lanes: Goat anti-rabbit, HRP labelled. at 1/2000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa, 60 kDa, 75 kDa
![Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808), expandable thumbnail](https://content.abcam.com/products/images/vitronectin-s-protein-antibody-ep781y-ab46808--immunoprecipitation-img6105.jpg "Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)")
Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)
Purified ab46808 at 1/50 dilution (2μg) immunoprecipitating Vitronectin/S-Protein in Human serum.
Lane 1 (input): Human serum 10μg
Lane 2 (+): ab46808 + Human serum.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab46808 in Human serum.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/10,000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 65-75 kDaAll lanes: Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)
Predicted band size: 54 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808), expandable thumbnail](https://content.abcam.com/products/images/vitronectin-s-protein-antibody-ep781y-ab46808--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73787.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)
This image shows human lung tissue treated with ab46808 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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