AB46808

Anti-Vitronectin/S-Protein antibody [EP781Y]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal Vitronectin/S-Protein antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Vitronectin, VN, S-protein, Serum-spreading factor, V75, VTN

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

This image shows human lung tissue treated with ab46808 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Lab

Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

Purified ab46808 at 1/50 dilution (2μg) immunoprecipitating Vitronectin/S-Protein in Human serum.
Lane 1 (input) : Human serum 10μg
Lane 2 (+) : ab46808 + Human serum.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab46808 in Human serum.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/10,000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 65-75 kDa

All lanes:

Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)

Predicted band size: 54 kDa

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Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

All lanes:

Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808) at 1/20000 dilution

All lanes:

Human serum at 10 µg

Secondary

All lanes:

Goat anti-rabbit, HRP labelled. at 1/2000 dilution

Predicted band size: 54 kDa

Observed band size: 54 kDa,60 kDa,75 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP781Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

This antibody has not been purified. It is provided in tissue culture supernatant and storage buffer.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.. Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.

See full target information Vitronectin

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

The American journal of pathology 194:180-194 PubMed38029923

2023

Multilamellated Basement Membranes in the Capillary Network of Alveolar Capillary Dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Jan C Kamp,Lavinia Neubert,Jonas C Schupp,Peter Braubach,Christoph Wrede,Florian Laenger,Tim Salditt,Jakob Reichmann,Tobias Welte,Arjang Ruhparwar,Fabio Ius,Nicolaus Schwerk,Anke K Bergmann,Sandra von Hardenberg,Matthias Griese,Christina Rapp,Karen M Olsson,Jan Fuge,Da-Hee Park,Marius M Hoeper,Danny D Jonigk,Lars Knudsen,Mark P Kuehnel

Endocrine journal 68:543-552 PubMed33473055

2021

Circular RNA circ_0008274 enhances the malignant progression of papillary thyroid carcinoma via modulating solute carrier family 7 member 11 by sponging miR-154-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Ma,Zhenghua Kan

Ultrasound in medicine & biology 45:3102-3115 PubMed31537389

2019

Mechanical Weakness of Thoracic Aorta Related to Aging or Dissection Predicted by Speed of Sound with Collagenase.

Applications

Unspecified application

Species

Unspecified reactive species

Katsutoshi Miura,Kanna Yamashita

International journal of clinical and experimental 8:11364-71 PubMed26617861

2015

Vitronectin significantly influences prognosis in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Shi,Rui-Long Lan,Xuan Tao,Chao-Yang Wu,Hai-Feng Hong,Jian-Hua Lin

Molecular medicine reports 7:53-8 PubMed23124223

2012

Increased expression levels of vitronectin in the maternal‑fetal interface of placenta in early-onset severe preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Shen,Xiaoyan Ma,Fei Yi,Azhar Rasul,Manhua Cui,Tonghui Ma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Vitronectin/S-Protein antibody [EP781Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (5)

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