Anti-Vitronectin/S-Protein antibody [EP781Y] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] - BSA and Azide free (AB247306)

This data was developed using ab46808, the same antibody clone in a different buffer formulation.

This image shows human lung tissue treated with ab46808 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IP

Lab

Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] - BSA and Azide free (AB247306)

This data was developed using ab46808, the same antibody clone in a different buffer formulation.
Purified ab46808 at 1/50 dilution (2μg) immunoprecipitating Vitronectin/S-Protein in Human serum.
Lane 1 (input) : Human serum 10μg
Lane 2 (+) : ab46808 + Human serum.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab46808 in Human serum.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/10,000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 65-75 kDa

All lanes:

Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (<a href='/en-us/products/primary-antibodies/vitronectin-s-protein-antibody-ep781y-ab46808'>ab46808</a>)

Predicted band size: 54 kDa

false

• WB

Unknown

Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] - BSA and Azide free (AB247306)

This data was developed using ab46808, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (<a href='/en-us/products/primary-antibodies/vitronectin-s-protein-antibody-ep781y-ab46808'>ab46808</a>) at 1/20000 dilution

All lanes:

Human serum at 10 µg

Secondary

All lanes:

Goat anti-rabbit, HRP labelled. at 1/2000 dilution

Predicted band size: 54 kDa

Observed band size: 54 kDa,60 kDa,75 kDa

false