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AB271837

Anti-Vitronectin/S-Protein antibody [EP873Y] - BSA and Azide free

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Rabbit Recombinant Monoclonal Vitronectin/S-Protein antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples.

View Alternative Names

Vitronectin, VN, S-protein, Serum-spreading factor, V75, VTN

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Vitronectin/S-Protein antibody [EP873Y] - BSA and Azide free (AB271837)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Vitronectin/S-Protein antibody [EP873Y] - BSA and Azide free (AB271837)

This data was developed using the same antibody clone in a different buffer formulation (ab45139).
Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) labeling Vitronectin/S-Protein with purified ab45139 at 1/100 dilution (7 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.11 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control : PBS instead of the primary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP873Y] - BSA and Azide free (AB271837)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP873Y] - BSA and Azide free (AB271837)

ab45139 at a 1/100 dilution, staining human Vitronectin/S-Protein in human liver tissue by immunohistochemistry using paraffin embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45139).

  • Unconjugated

    Anti-Vitronectin/S-Protein antibody [EP873Y]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Vitronectin/S-Protein antibody [EP873Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Vitronectin/S-Protein antibody [EP873Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Vitronectin/S-Protein antibody [EP873Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Vitronectin/S-Protein antibody [EP873Y]

  • 578 PE

    PE Anti-Vitronectin/S-Protein antibody [EP873Y]

  • 660 APC

    APC Anti-Vitronectin/S-Protein antibody [EP873Y]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Vitronectin/S-Protein antibody [EP873Y]

  • HRP

    HRP Anti-Vitronectin/S-Protein antibody [EP873Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP873Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab271837 is the carrier-free version of ab45139.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.. Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
See full target information Vitronectin
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Alternative Products

Primary Antibodies

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com