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AB251460

Anti-VKORC1 antibody [EPR20245] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal VKORC1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

VKOR, MSTP134, MSTP576, UNQ308/PRO351, VKORC1, Vitamin K epoxide reductase complex subunit 1

8 Images
Immunocytochemistry/ Immunofluorescence - Anti-VKORC1 antibody [EPR20245] - BSA and Azide free (AB251460)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VKORC1 antibody [EPR20245] - BSA and Azide free (AB251460)

This data was developed using ab206656, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells labeling VKORC1 with ab206656 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on A549 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-VKORC1 antibody [EPR20245] - BSA and Azide free (AB251460)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-VKORC1 antibody [EPR20245] - BSA and Azide free (AB251460)

This data was developed using ab206656, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling VKORC1 with ab206656 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-VKORC1 antibody [EPR20245] - BSA and Azide free (AB251460)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VKORC1 antibody [EPR20245] - BSA and Azide free (AB251460)

This data was developed using ab206656, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling VKORC1 with ab206656 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Immunoprecipitation - Anti-VKORC1 antibody [EPR20245] - BSA and Azide free (AB251460)
  • IP

Supplier Data

Immunoprecipitation - Anti-VKORC1 antibody [EPR20245] - BSA and Azide free (AB251460)

This data was developed using ab206656, the same antibody clone in a different buffer formulation.

VKORC1 was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab206656 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab206656 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HepG2 whole cell lysate, 10 μg (Input).

Lane 2 : ab206656 IP in HepG2 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab206656 in HepG2 whole cell lysate.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-VKORC1 antibody [EPR20245] (<a href='/en-us/products/primary-antibodies/vkorc1-antibody-epr20245-ab206656'>ab206656</a>)

Predicted band size: 18 kDa

Observed band size: 15 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VKORC1 antibody [EPR20245] - BSA and Azide free (AB251460)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VKORC1 antibody [EPR20245] - BSA and Azide free (AB251460)

This data was developed using ab206656, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling VKORC1 with ab206656 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on hepatocytes of mouse liver is observed [PMID : 25747820] [PMID : 14765194]. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-VKORC1 antibody [EPR20245] - BSA and Azide free (AB251460)
  • WB

Supplier Data

Western blot - Anti-VKORC1 antibody [EPR20245] - BSA and Azide free (AB251460)

This data was developed using ab206656, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-VKORC1 antibody [EPR20245] (<a href='/en-us/products/primary-antibodies/vkorc1-antibody-epr20245-ab206656'>ab206656</a>) at 1/1000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 3:

Mouse liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 18 kDa

Observed band size: 15 kDa

false

Exposure time: 8s

Western blot - Anti-VKORC1 antibody [EPR20245] - BSA and Azide free (AB251460)
  • WB

Unknown

Western blot - Anti-VKORC1 antibody [EPR20245] - BSA and Azide free (AB251460)

This data was developed using ab206656, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 25753038).

All lanes:

Western blot - Anti-VKORC1 antibody [EPR20245] (<a href='/en-us/products/primary-antibodies/vkorc1-antibody-epr20245-ab206656'>ab206656</a>) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 18 kDa

Observed band size: 15 kDa

false

Exposure time: 3min

Western blot - Anti-VKORC1 antibody [EPR20245] - BSA and Azide free (AB251460)
  • WB

Supplier Data

Western blot - Anti-VKORC1 antibody [EPR20245] - BSA and Azide free (AB251460)

This data was developed using ab206656, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-VKORC1 antibody [EPR20245] (<a href='/en-us/products/primary-antibodies/vkorc1-antibody-epr20245-ab206656'>ab206656</a>) at 1/1000 dilution

Lane 1:

Mouse lung lysate at 10 µg

Lane 2:

Mouse pancreas lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 18 kDa

Observed band size: 15 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20245

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is unsuitable for IHC in human.

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Reagents, Controls & Accessories

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TMB substrate 1X

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>This antibody is unsuitable for IHC in human.</p>" } } }
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Product details

ab251460 is the carrier-free version of ab206656.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vitamin K epoxide reductase complex subunit 1 (VKORC1) also known as VKOR is an enzyme that plays an important role in the vitamin K cycle. This enzyme has a molecular mass of approximately 18 kDa. VKORC1 helps in reducing vitamin K 23-epoxide to its active hydroquinone form which is necessary for the gamma-carboxylation of vitamin K-dependent proteins. These proteins are mainly responsible for blood coagulation. VKORC1 expression is high in the liver where much of the synthesis of clotting factors occurs.
Biological function summary

VKORC1 is integral to the vitamin K cycle an essential process for activating vitamin K-dependent clotting factors. It operates within a complex with its protein partner VKORC1-like 1 (VKORC1L1). This partnership is important for maintaining effective blood coagulation. The active form of vitamin K produced by VKORC1 ensures the activity of several blood clotting factors especially factors II VII IX and X. Without proper function of VKORC1 these factors would remain inactive.

Pathways

The vitamin K cycle is where VKORC1 makes a significant impact and this cycle is vital for blood coagulation processes. Specifically VKORC1 reduces vitamin K epoxide working closely with gamma-glutamyl carboxylase (GGCX) another enzyme that modifies the glutamic acid residues on clotting factors. This modification is important for their proper functioning within the coagulation cascade. The interplay between VKORC1 and GGCX directly connects to the synthesis of active blood coagulation proteins.

VKORC1 mutations are notably linked to warfarin resistance and susceptibility to bleeding disorders. Warfarin is an anticoagulant that targets VKORC1 to prevent clot formation and variations in this protein can lead to altered drug response. Furthermore any dysfunction in VKORC1 may result in vitamin K epoxide reductase deficiency affecting coagulation and leading to increased bleeding risk. Mutations could also affect partner proteins like coagulation factors II and VII leading to disorders related to abnormal blood clotting processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in vitamin K metabolism. Catalytic subunit of the vitamin K epoxide reductase (VKOR) complex which reduces inactive vitamin K 2,3-epoxide to active vitamin K. Vitamin K is required for the gamma-carboxylation of various proteins, including clotting factors, and is required for normal blood coagulation, but also for normal bone development.
See full target information VKORC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:26521 PubMed39489850

2024

GABPA inhibits tumorigenesis in clear cell renal cell carcinoma by regulating ferroptosis through ACSL4.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqian Wu,Zonglong Wu,Mengfei Yao,Li Liu,Yimeng Song,Lulin Ma,Cheng Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com