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AB113684

Anti-VMA2 antibody [13D11B2]

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(3 Publications)

Mouse Monoclonal VMA2 antibody. Suitable for WB, ICC/IF and reacts with Saccharomyces cerevisiae samples. Cited in 3 publications.

View Alternative Names

VAT2, YBR127C, YBR1002, VMA2, V-type proton ATPase subunit B, V-ATPase subunit B, V-ATPase 57 kDa subunit, Vacuolar proton pump subunit B

1 Images
Western blot - Anti-VMA2 antibody [13D11B2] (AB113684)
  • WB

Unknown

Western blot - Anti-VMA2 antibody [13D11B2] (AB113684)

All lanes:

Western blot - Anti-VMA2 antibody [13D11B2] (ab113684)

All lanes:

Yeast whole cell lysate at 15 µg

Predicted band size: 58 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

13D11B2

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Saccharomyces cerevisiae

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.25-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification technique
Proprietary technique
Purification notes
Near homogeneity as judged by SDS-PAGE (purity >95%). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VMA2 also known as V-type proton ATPase subunit B enzyme plays a role in the assembly and function of the V-ATPase complex that acidifies cellular compartments. This protein has a mass of approximately 57 kDa. VMA2 localizes to membrane structures and is expressed highly in yeast and other eukaryotic organisms. Its main role is maintaining the proton gradient across the vacuolar membrane influencing several cellular processes.
Biological function summary

VMA2 functions as part of the V-ATPase complex which is essential for cellular energy regulation and the acidification of vacuoles and endosomes. This complex contributes to cellular homeostasis by facilitating pH regulation and ion transport. VMA2 as a non-catalytic subunit assists with the stabilization of the enzyme structure and participates in proton transport processes that impact numerous cellular activities.

Pathways

VMA2 influences protein sorting and vesicle trafficking within the endocytic and secretory pathways. It collaborates with proteins like VMA9 and VMA13 to regulate these processes. The function of the V-ATPase complex including VMA2 integrates into pathways like the pH signaling pathway and vesicular trafficking ensuring proper cellular functions like nutrient uptake and protein degradation.

VMA2 has been linked to conditions like osteopetrosis and Fanconi syndrome. These disorders relate to improper acidification due to dysfunctional V-ATPase. VMA2's association with VMA4 and other V-ATPase components becomes important in understanding the molecular basis of these diseases shedding light on possible therapeutic targets or disease markers.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed : 2141385). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments (PubMed : 2141385).
See full target information VMA2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biomaterials research 29:0201 PubMed40330275

2025

Impact of Culture Duration on the Properties and Functionality of Yeast-Derived Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Gyeongchan Jeon,Yang-Hoon Kim,Jiho Min

iScience 24:103338 PubMed34805795

2021

The fission yeast FLCN/FNIP complex augments TORC1 repression or activation in response to amino acid (AA) availability.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel A Calvo,Shalini Sharma,Joao A Paulo,Alexander O D Gulka,Andras Boeszoermenyi,Jingyu Zhang,Jose M Lombana,Christina M Palmieri,Laura A Laviolette,Haribabu Arthanari,Othon Iliopoulos,Steven P Gygi,Mo Motamedi

Autophagy 13:114-132 PubMed27846375

2016

Ubiquitination of ERMES components by the E3 ligase Rsp5 is involved in mitophagy.

Applications

WB

Species

Saccharomyces cerevisiae S288C

Naïma Belgareh-Touzé,Laetitia Cavellini,Mickael M Cohen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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