Rabbit Recombinant Monoclonal VMAT1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-VMAT1 antibody [EPR6476] - BSA and Azide free (AB249447), expandable thumbnail](https://content.abcam.com/products/images/vmat1-antibody-epr6476-bsa-and-azide-free-ab249447--flow-cytometry-intracellular-img80066.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-VMAT1 antibody [EPR6476] - BSA and Azide free (AB249447)")
![Western blot - Anti-VMAT1 antibody [EPR6476] - BSA and Azide free (AB249447), expandable thumbnail](https://content.abcam.com/products/images/vmat1-antibody-epr6476-bsa-and-azide-free-ab249447--western-blot-img126798.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VMAT1 antibody [EPR6476] - BSA and Azide free (AB249447)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Predicted | Not recommended | Not recommended | Predicted |
| Rat | Not recommended | Tested | Not recommended | Not recommended | Expected |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Isoform 1. Electrogenic antiporter that exchanges one cationic monoamine with two intravesicular protons across the membrane of secretory and synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to accumulate high concentrations of monoamines inside the vesicles prior to their release via exocytosis. Transports catecholamines and indolamines with higher affinity for serotonin (PubMed:16326835, PubMed:23337945, PubMed:8643547). Regulates the transvesicular monoaminergic gradient that determines the quantal size. Mediates presynaptic monoaminergic vesicle transport in the amygdala and prefrontal brain regions related with emotion processing in response to environmental stimuli (PubMed:23337945). Isoform 2. Unable to uptake serotonin.
Alternative names
VAT1, VMAT1, SLC18A1, Chromaffin granule amine transporter, Solute carrier family 18 member 1, Vesicular amine transporter 1
Recommended products
Rabbit Recombinant Monoclonal VMAT1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6476
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249447 is the carrier-free version of Anti-VMAT1 antibody [EPR6476] ab168347.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Vesicular monoamine transporter 1 (VMAT1) also known as solute carrier family 18 member 1 (SLC18A1) facilitates the transport of monoamines—such as dopamine serotonin and norepinephrine—into synaptic vesicles. VMAT1 with a molecular weight of approximately 46 kDa is particularly expressed in neuroendocrine cells. These are present in tissues like the adrenal medulla and various parts of the central nervous system. Its transporter function is critical for monoamine storage and release essentially influencing neurotransmitter recycling.
- Biological function summary
VMAT1 is involved in the modulation of neural signaling processes. It functions as a monoamine transporter by regulating neurotransmitter levels within vesicles preparing them for secretion. By doing so it influences synaptic transmission and signal transduction impacting mood cognition and other neural activities. VMAT1 operates as part of a vesicular transport machinery functioning alongside other proteins that maintain neurotransmitter balance.
- Pathways
VMAT1 plays an important role in the catecholamine and serotonin neurotransmitter pathways. It associates with proteins like dopamine transporter (DAT) and serotonin transporter (SERT) to maintain neurotransmitter homeostasis. These pathways are integral for mood regulation and stress responses. Interactions within these pathways are vital for maintaining synaptic stability and efficient neurotransmitter exchange.
- Associated diseases and disorders
VMAT1 has been implicated in neuropsychiatric conditions such as schizophrenia and mood disorders. Altered VMAT1 function may disrupt monoamine storage and release contributing to imbalances observed in these disorders. The interaction with related proteins such as DAT highlights its role in dopamine-related dysfunction in these diseases. Understanding VMAT1's involvement in these conditions aids the development of targeted therapies to correct neurotransmitter imbalances.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry (Intracellular) - Anti-VMAT1 antibody [EPR6476] - BSA and Azide free (ab249447), expandable thumbnail](https://content.abcam.com/products/images/vmat1-antibody-epr6476-bsa-and-azide-free-ab249447--flow-cytometry-intracellular-img80066.jpg "Flow Cytometry (Intracellular) - Anti-VMAT1 antibody [EPR6476] - BSA and Azide free (ab249447)")
Flow Cytometry (Intracellular) - Anti-VMAT1 antibody [EPR6476] - BSA and Azide free (ab249447)
This data was developed using Anti-VMAT1 antibody [EPR6476] ab168347, the same antibody clone in a different buffer formulation.
Intracellular flow cytometrical analysis of permeabilized A549 cells with Anti-VMAT1 antibody [EPR6476] ab168347 antibody at a dilution of 1/100 (red) or a rabbit IgG negative (green).
![Western blot - Anti-VMAT1 antibody [EPR6476] - BSA and Azide free (ab249447), expandable thumbnail](https://content.abcam.com/products/images/vmat1-antibody-epr6476-bsa-and-azide-free-ab249447--western-blot-img126798.jpg "Western blot - Anti-VMAT1 antibody [EPR6476] - BSA and Azide free (ab249447)")
Western blot - Anti-VMAT1 antibody [EPR6476] - BSA and Azide free (ab249447)
This data was developed using Anti-VMAT1 antibody [EPR6476] ab168347, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-VMAT1 antibody [EPR6476] (Anti-VMAT1 antibody [EPR6476] ab168347) at 1/1000 dilution
Lane 1: BxPC-3 cell lysate at 10 µg
Lane 2: PC-12 cell lysate at 10 µg
Lane 3: A549 cell lysate at 10 µg
Predicted band size: 56 kDa
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