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AB191121

Anti-VMAT2 antibody

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(7 Publications)

Rabbit Polyclonal VMAT2 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

View Alternative Names

SVMT, VMAT2, SLC18A2, Synaptic vesicular amine transporter, Solute carrier family 18 member 2, Vesicular amine transporter 2, Vesicular monoamine transporter 2, VAT2

3 Images
Western blot - Anti-VMAT2 antibody (AB191121)
  • WB

Supplier Data

Western blot - Anti-VMAT2 antibody (AB191121)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-VMAT2 antibody (ab191121) at 1/1000 dilution

All lanes:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 56 kDa

Observed band size: 56 kDa

false

Western blot - Anti-VMAT2 antibody (AB191121)
  • WB

Supplier Data

Western blot - Anti-VMAT2 antibody (AB191121)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-VMAT2 antibody (ab191121) at 1/1000 dilution

All lanes:

Mouse brain lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa

Observed band size: 56 kDa

false

Western blot - Anti-VMAT2 antibody (AB191121)
  • WB

Supplier Data

Western blot - Anti-VMAT2 antibody (AB191121)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-VMAT2 antibody (ab191121) at 1/200 dilution

All lanes:

Rat brain lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa

Observed band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The vesicular monoamine transporter 2 (VMAT2) also known as SLC18A2 is a protein that is important for transporting neurotransmitters like dopamine serotonin norepinephrine and histamine into synaptic vesicles. VMAT2 has a mass of approximately 70 kDa and is mainly found in the brain where it is expressed in neurons. Its activity relies on the proton gradient across the vesicular membrane enabling it to concentrate neurotransmitters in vesicles for release into the synaptic cleft.
Biological function summary

VMAT2 handles the packaging and storage of important monoamine neurotransmitters which play a role in mood regulation cognition and movement. Although VMAT2 acts independently it is important for the functioning of neurotransmitter transport and release. By storing neurotransmitters into vesicles VMAT2 ensures that neurons can quickly release them into the synapse when needed supporting efficient neural signaling.

Pathways

VMAT2 plays an important role in the dopamine and serotonin systems. In the dopamine pathway VMAT2 stores dopamine into vesicles which are then released during neurotransmission. It works closely with the dopamine transporter DAT which reuptakes dopamine from the synaptic cleft back into neurons. Similarly in the serotonin pathway VMAT2 collaborates with the serotonin transporter SERT. These relationships emphasize VMAT2's central position in modulating neurotransmitter signaling linking it to mood and motor control regulation.

VMAT2 has a notable link to neurological and psychiatric conditions. Parkinson's disease characterized by the loss of dopaminergic neurons shows disrupted VMAT2 function affecting dopamine storage and release. In depression altered monoamine neurotransmitter levels including serotonin and dopamine involve VMAT2 dysfunction. The protein is not only connected to these diseases through its own function but also through its interactions with DAT and SERT which are key players in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Electrogenic antiporter that exchanges one cationic monoamine with two intravesicular protons across the membrane of secretory and synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to accumulate high concentrations of monoamines inside the vesicles prior to their release via exocytosis. Transports a variety of catecholamines such as dopamine, adrenaline and noradrenaline, histamine, and indolamines such as serotonin (PubMed : 23363473, PubMed : 37914936, PubMed : 38081299, PubMed : 38517752, PubMed : 8643547). Regulates the transvesicular monoaminergic gradient that determines the quantal size. Mediates somatodendritic dopamine release in hippocampal neurons, likely as part of a regulated secretory pathway that integrates retrograde synaptic signals (By similarity). Acts as a primary transporter for striatal dopamine loading ensuring impulse-dependent release of dopamine at the synaptic cleft (By similarity). Responsible for histamine and serotonin storage and subsequent corelease from mast cell granules (PubMed : 8860238).
See full target information SLC18A2
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Pharmacology research & perspectives 13:e70057 PubMed39806528

2025

HDAC4/5 Inhibitor, LMK-235 Improves Animal Voluntary Movement in MPTP-Induced Parkinson's Disease Model.

Applications

Unspecified application

Species

Unspecified reactive species

Heejin Lee,Hyun-Jin Kim,Ju-Sik Min,Eunhye Lee,Dong Kyu Choi,Jae-Hyeog Choi,Yohan Seo,Sion Lee,Chun Young Im,Gi Hun Bae,Yoojin Oh,Eun-A Ko,Sung-Cherl Jung,Soong-Hyun Kim,Oh-Bin Kwon

Pharmacology research & perspectives 11:e01135 PubMed37740715

2023

Dopaminergic cell protection and alleviation of neuropsychiatric disease symptoms by VMAT2 expression through the class I HDAC inhibitor TC-H 106.

Applications

Unspecified application

Species

Unspecified reactive species

Heejin Lee,Hye-Ji Kim,Dong-Kyu Choi,Eu N-A Ko,Jae-Hyeog Choi,Yohan Seo,Sion Lee,Soong-Hyun Kim,Sejin Jung,Minwoo Kim,Dongwan Kang,Chun-Young Im,Gi-Hun Bae,Sung-Cherl Jung,Oh-Bin Kwon

NPJ Parkinson's disease 9:135 PubMed37726343

2023

Cortical Lewy body injections induce long-distance pathogenic alterations in the non-human primate brain.

Applications

Unspecified application

Species

Unspecified reactive species

Margaux Teil,Sandra Dovero,Mathieu Bourdenx,Marie-Laure Arotcarena,Morgane Darricau,Gregory Porras,Marie-Laure Thiolat,Inés Trigo-Damas,Celine Perier,Cristina Estrada,Nuria Garcia-Carrillo,María Trinidad Herrero,Miquel Vila,José A Obeso,Erwan Bezard,Benjamin Dehay

NPJ Parkinson's disease 9:56 PubMed37029193

2023

Aberrant somatic calcium channel function in cNurr1 and LRRK2-G2019S mice.

Applications

Unspecified application

Species

Unspecified reactive species

Olga Skiteva,Ning Yao,Ioannis Mantas,Xiaoqun Zhang,Thomas Perlmann,Per Svenningsson,Karima Chergui

The European journal of neuroscience 48:3514-3533 PubMed30402991

2018

Adaptive neurogenesis in the cerebral cortex and contralateral subventricular zone induced by unilateral cortical devascularization: Possible modulation by dopamine neurotransmission.

Applications

Unspecified application

Species

Unspecified reactive species

Leslie Vargas-Saturno,Carlos Ayala-Grosso

Frontiers in neuroscience 12:545 PubMed30147642

2018

Glycosylation States of Pre- and Post-synaptic Markers of 5-HT Neurons Differ With Sex and 5-HTTLPR Genotype in Cortical Autopsy Samples.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer N K Nyarko,Maa O Quartey,Ryan M Heistad,Paul R Pennington,Lisa J Poon,Kaeli J Knudsen,Odette Allonby,Amr M El Zawily,Andrew Freywald,Gail Rauw,Glen B Baker,Darrell D Mousseau

eNeuro 5: PubMed29911172

2018

PICK1-Deficient Mice Exhibit Impaired Response to Cocaine and Dysregulated Dopamine Homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Kathrine Louise Jensen,Gunnar Sørensen,Ditte Dencker,William Anthony Owens,Troels Rahbek-Clemmensen,Michael Brett Lever,Annika H Runegaard,Nikolaj Riis Christensen,Pia Weikop,Gitta Wörtwein,Anders Fink-Jensen,Kenneth L Madsen,Lynette Daws,Ulrik Gether,Mattias Rickhag
View all publications
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