AB26073

Anti-VMAT2 antibody

Name: Anti-VMAT2 antibody (ab26073) | Abcam
Brand: Abcam Limited
SKU: AB26073

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(1 Publication)

Goat Polyclonal VMAT2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC18A2 aa 500 to C-terminus.

View Alternative Names

SVMT, VMAT2, SLC18A2, Synaptic vesicular amine transporter, Solute carrier family 18 member 2, Vesicular amine transporter 2, Vesicular monoamine transporter 2, VAT2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody (AB26073)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody (AB26073)

Immunohistochemistry staining of paraffin embedded Human Adrenal Gland labeling VMAT2 with ab26073 at (6µg/mL). Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Supplier Data

Western blot - Anti-VMAT2 antibody (AB26073)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-VMAT2 antibody (ab26073) at 2 µg/mL

All lanes:

Human Adrenal Gland lysate at 35 µg

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SLC18A2 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q05940

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "6 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The vesicular monoamine transporter 2 (VMAT2) also known as SLC18A2 is a protein that is important for transporting neurotransmitters like dopamine serotonin norepinephrine and histamine into synaptic vesicles. VMAT2 has a mass of approximately 70 kDa and is mainly found in the brain where it is expressed in neurons. Its activity relies on the proton gradient across the vesicular membrane enabling it to concentrate neurotransmitters in vesicles for release into the synaptic cleft.

Biological function summary

VMAT2 handles the packaging and storage of important monoamine neurotransmitters which play a role in mood regulation cognition and movement. Although VMAT2 acts independently it is important for the functioning of neurotransmitter transport and release. By storing neurotransmitters into vesicles VMAT2 ensures that neurons can quickly release them into the synapse when needed supporting efficient neural signaling.

Pathways

VMAT2 plays an important role in the dopamine and serotonin systems. In the dopamine pathway VMAT2 stores dopamine into vesicles which are then released during neurotransmission. It works closely with the dopamine transporter DAT which reuptakes dopamine from the synaptic cleft back into neurons. Similarly in the serotonin pathway VMAT2 collaborates with the serotonin transporter SERT. These relationships emphasize VMAT2's central position in modulating neurotransmitter signaling linking it to mood and motor control regulation.

VMAT2 has a notable link to neurological and psychiatric conditions. Parkinson's disease characterized by the loss of dopaminergic neurons shows disrupted VMAT2 function affecting dopamine storage and release. In depression altered monoamine neurotransmitter levels including serotonin and dopamine involve VMAT2 dysfunction. The protein is not only connected to these diseases through its own function but also through its interactions with DAT and SERT which are key players in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Electrogenic antiporter that exchanges one cationic monoamine with two intravesicular protons across the membrane of secretory and synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to accumulate high concentrations of monoamines inside the vesicles prior to their release via exocytosis. Transports a variety of catecholamines such as dopamine, adrenaline and noradrenaline, histamine, and indolamines such as serotonin (PubMed : 23363473, PubMed : 37914936, PubMed : 38081299, PubMed : 38517752, PubMed : 8643547). Regulates the transvesicular monoaminergic gradient that determines the quantal size. Mediates somatodendritic dopamine release in hippocampal neurons, likely as part of a regulated secretory pathway that integrates retrograde synaptic signals (By similarity). Acts as a primary transporter for striatal dopamine loading ensuring impulse-dependent release of dopamine at the synaptic cleft (By similarity). Responsible for histamine and serotonin storage and subsequent corelease from mast cell granules (PubMed : 8860238).

See full target information SLC18A2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 284:26964-77 PubMed19643733

2009

SLIMMER (FHL1B/KyoT3) interacts with the proapoptotic protein Siva-1 (CD27BP) and delays skeletal myoblast apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Denny L Cottle,Meagan J McGrath,Brendan R Wilding,Belinda S Cowling,Jordan M Kane,Colleen E D'Arcy,Melissa Holdsworth,Irene Hatzinisiriou,Mark Prescott,Susan Brown,Christina A Mitchell

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-VMAT2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody (AB26073)

Western blot - Anti-VMAT2 antibody (AB26073)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise