AB280364

Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal VMAT2 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Rat, Mouse samples.

View Alternative Names

Vmat2, Slc18a2, Synaptic vesicular amine transporter, Monoamine transporter, Solute carrier family 18 member 2, Vesicular amine transporter 2, Vesicular monoamine transporter 2, VAT2

11 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

This data was developed using ab259970, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse skin tissue labeling VMAT2 with ab259970 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : almost no staining on mouse skin.The section was incubated with ab259970 for 30 minutes at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

This data was developed using ab259970, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat skin tissue labeling VMAT2 with ab259970 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : almost no staining on rat skin. The section was incubated with ab259970 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

This data was developed using ab259970, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling VMAT2 with ab259970 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum (PMID : 27836486). The section was incubated with ab259970 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

This data was developed using ab259970, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling VMAT2 with ab259970 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : almost no staining on mouse spleen.The section was incubated with ab259970 for 30 minutes at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

This data was developed using ab259970, the same antibody clone in a different buffer formulation.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

This data was developed using ab259970, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling VMAT2 with ab259970 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum. The section was incubated with ab259970 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

This data was developed using ab259970, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling VMAT2 with ab259970 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : almost no staining on rat spleen.The section was incubated with ab259970 for 30 minutes at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

Supplier Data

Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

This data was developed using ab259970, the same antibody clone in a different buffer formulation.

VMAT2 was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 ug with ab259970 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259970 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat brain tissue lysate 10 ug

Lane 2 : ab259970 IP in Rat brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab259970 in Rat brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds

Input sample is non-boiled as boiling may cause protein aggregates.

All lanes:

Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] (<a href='/en-us/products/primary-antibodies/vmat2-antibody-epr24197-51-ab259970'>ab259970</a>)

Predicted band size: 56 kDa

Observed band size: 55 kDa

false

Supplier Data

Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

This data was developed using ab259970, the same antibody clone in a different buffer formulation.

VMAT2 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab259970 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259970 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse brain tissue lysate 10 ug

Lane 2 : ab259970 IP in Mouse brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab259970 in Mouse brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds

Input sample is non-boiled as boiling may cause protein aggregates.

All lanes:

Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] (<a href='/en-us/products/primary-antibodies/vmat2-antibody-epr24197-51-ab259970'>ab259970</a>)

Predicted band size: 56 kDa

Observed band size: 55 kDa

false

Lab

Western blot - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

This data was developed using ab259970, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Samples are non-boiled as boiling may cause protein aggregates.

This protein is specifically expressed in CNS.

Exposure time : 48 seconds

All lanes:

Western blot - Anti-VMAT2 antibody [EPR24197-51] (<a href='/en-us/products/primary-antibodies/vmat2-antibody-epr24197-51-ab259970'>ab259970</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse hippocampus tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Mouse skin tissue lysate at 20 µg

Lane 5:

Mouse thyroid tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 56 kDa

Observed band size: 55 kDa

false

Lab

Western blot - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

This data was developed using ab259970, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Samples are non-boiled as boiling may cause protein aggregates.

This protein is specifically expressed in CNS.

Exposure time : Lane 1 : 29 seconds

Lane 2-5 : 59 seconds

All lanes:

Western blot - Anti-VMAT2 antibody [EPR24197-51] (<a href='/en-us/products/primary-antibodies/vmat2-antibody-epr24197-51-ab259970'>ab259970</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat hippocampus tissue lysate at 20 µg

Lane 3:

Rat spleen tissue lysate at 20 µg

Lane 4:

Rat skin tissue lysate at 20 µg

Lane 5:

Rat thyroid tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 56 kDa

Observed band size: 55 kDa

false

Unconjugated

Anti-VMAT2 antibody [EPR24197-51]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24197-51

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

ICC/IF, IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab280364 is the carrier-free version of ab259970.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The vesicular monoamine transporter 2 (VMAT2) also known as SLC18A2 is a protein that is important for transporting neurotransmitters like dopamine serotonin norepinephrine and histamine into synaptic vesicles. VMAT2 has a mass of approximately 70 kDa and is mainly found in the brain where it is expressed in neurons. Its activity relies on the proton gradient across the vesicular membrane enabling it to concentrate neurotransmitters in vesicles for release into the synaptic cleft.

Biological function summary

VMAT2 handles the packaging and storage of important monoamine neurotransmitters which play a role in mood regulation cognition and movement. Although VMAT2 acts independently it is important for the functioning of neurotransmitter transport and release. By storing neurotransmitters into vesicles VMAT2 ensures that neurons can quickly release them into the synapse when needed supporting efficient neural signaling.

Pathways

VMAT2 plays an important role in the dopamine and serotonin systems. In the dopamine pathway VMAT2 stores dopamine into vesicles which are then released during neurotransmission. It works closely with the dopamine transporter DAT which reuptakes dopamine from the synaptic cleft back into neurons. Similarly in the serotonin pathway VMAT2 collaborates with the serotonin transporter SERT. These relationships emphasize VMAT2's central position in modulating neurotransmitter signaling linking it to mood and motor control regulation.

VMAT2 has a notable link to neurological and psychiatric conditions. Parkinson's disease characterized by the loss of dopaminergic neurons shows disrupted VMAT2 function affecting dopamine storage and release. In depression altered monoamine neurotransmitter levels including serotonin and dopamine involve VMAT2 dysfunction. The protein is not only connected to these diseases through its own function but also through its interactions with DAT and SERT which are key players in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Electrogenic antiporter that exchanges one cationic monoamine with two intravesicular protons across the membrane of secretory and synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to accumulate high concentrations of monoamines inside the vesicles prior to their release via exocytosis. Transports a variety of catecholamines such as dopamine, adrenaline and noradrenaline, histamine, and indolamines such as serotonin (By similarity) (PubMed : 10618388). Regulates the transvesicular monoaminergic gradient that determines the quantal size. Mediates somatodendritic dopamine release in hippocampal neurons, likely as part of a regulated secretory pathway that integrates retrograde synaptic signals (By similarity). Acts as a primary transporter for striatal dopamine loading ensuring impulse-dependent release of dopamine at the synaptic cleft (PubMed : 9427251). Responsible for histamine and serotonin storage and subsequent corelease from mast cell granules (By similarity) (PubMed : 10618388).

See full target information Slc18a2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

Western blot - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

Western blot - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise