Rabbit Recombinant Monoclonal VMAT2 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Rat, Mouse samples.
Images
![Western blot - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364), expandable thumbnail](https://content.abcam.com/products/images/vmat2-antibody-epr24197-51-bsa-and-azide-free-ab280364--western-blot-img7783.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)")
![Western blot - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364), expandable thumbnail](https://content.abcam.com/products/images/vmat2-antibody-epr24197-51-bsa-and-azide-free-ab280364--western-blot-img5392.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)")
![Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364), expandable thumbnail](https://content.abcam.com/products/images/vmat2-antibody-epr24197-51-bsa-and-azide-free-ab280364--immunoprecipitation-img138192.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364), expandable thumbnail](https://content.abcam.com/products/images/vmat2-antibody-epr24197-51-bsa-and-azide-free-ab280364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10422.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364), expandable thumbnail](https://content.abcam.com/products/images/vmat2-antibody-epr24197-51-bsa-and-azide-free-ab280364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118616.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (AB280364)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Tested | Tested | Not recommended | Tested |
| Rat | Tested | Tested | Tested | Not recommended | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Electrogenic antiporter that exchanges one cationic monoamine with two intravesicular protons across the membrane of secretory and synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to accumulate high concentrations of monoamines inside the vesicles prior to their release via exocytosis. Transports a variety of catecholamines such as dopamine, adrenaline and noradrenaline, histamine, and indolamines such as serotonin (By similarity) (PubMed:10618388). Regulates the transvesicular monoaminergic gradient that determines the quantal size. Mediates somatodendritic dopamine release in hippocampal neurons, likely as part of a regulated secretory pathway that integrates retrograde synaptic signals (By similarity). Acts as a primary transporter for striatal dopamine loading ensuring impulse-dependent release of dopamine at the synaptic cleft (PubMed:9427251). Responsible for histamine and serotonin storage and subsequent corelease from mast cell granules (By similarity) (PubMed:10618388).
Alternative names
Vmat2, Slc18a2, Synaptic vesicular amine transporter, Monoamine transporter, Solute carrier family 18 member 2, Vesicular amine transporter 2, Vesicular monoamine transporter 2, VAT2
Recommended products
Rabbit Recombinant Monoclonal VMAT2 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24197-51
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab280364 is the carrier-free version of Anti-VMAT2 antibody [EPR24197-51] ab259970.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The vesicular monoamine transporter 2 (VMAT2) also known as SLC18A2 is a protein that is important for transporting neurotransmitters like dopamine serotonin norepinephrine and histamine into synaptic vesicles. VMAT2 has a mass of approximately 70 kDa and is mainly found in the brain where it is expressed in neurons. Its activity relies on the proton gradient across the vesicular membrane enabling it to concentrate neurotransmitters in vesicles for release into the synaptic cleft.
- Biological function summary
VMAT2 handles the packaging and storage of important monoamine neurotransmitters which play a role in mood regulation cognition and movement. Although VMAT2 acts independently it is important for the functioning of neurotransmitter transport and release. By storing neurotransmitters into vesicles VMAT2 ensures that neurons can quickly release them into the synapse when needed supporting efficient neural signaling.
- Pathways
VMAT2 plays an important role in the dopamine and serotonin systems. In the dopamine pathway VMAT2 stores dopamine into vesicles which are then released during neurotransmission. It works closely with the dopamine transporter DAT which reuptakes dopamine from the synaptic cleft back into neurons. Similarly in the serotonin pathway VMAT2 collaborates with the serotonin transporter SERT. These relationships emphasize VMAT2's central position in modulating neurotransmitter signaling linking it to mood and motor control regulation.
- Associated diseases and disorders
VMAT2 has a notable link to neurological and psychiatric conditions. Parkinson's disease characterized by the loss of dopaminergic neurons shows disrupted VMAT2 function affecting dopamine storage and release. In depression altered monoamine neurotransmitter levels including serotonin and dopamine involve VMAT2 dysfunction. The protein is not only connected to these diseases through its own function but also through its interactions with DAT and SERT which are key players in these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
11 product images
![Western blot - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364), expandable thumbnail](https://content.abcam.com/products/images/vmat2-antibody-epr24197-51-bsa-and-azide-free-ab280364--western-blot-img7783.jpg "Western blot - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)")
Western blot - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
This data was developed using Anti-VMAT2 antibody [EPR24197-51] ab259970, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Samples are non-boiled as boiling may cause protein aggregates.
This protein is specifically expressed in CNS.
Exposure time: 48 seconds
All lanes: Western blot - Anti-VMAT2 antibody [EPR24197-51] (Anti-VMAT2 antibody [EPR24197-51] ab259970) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse hippocampus tissue lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
Lane 4: Mouse skin tissue lysate at 20 µg
Lane 5: Mouse thyroid tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 56 kDa
Observed band size: 55 kDa
![Western blot - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364), expandable thumbnail](https://content.abcam.com/products/images/vmat2-antibody-epr24197-51-bsa-and-azide-free-ab280364--western-blot-img5392.jpg "Western blot - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)")
Western blot - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
This data was developed using Anti-VMAT2 antibody [EPR24197-51] ab259970, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Samples are non-boiled as boiling may cause protein aggregates.
This protein is specifically expressed in CNS.
Exposure time: Lane 1: 29 seconds
Lane 2-5: 59 seconds
All lanes: Western blot - Anti-VMAT2 antibody [EPR24197-51] (Anti-VMAT2 antibody [EPR24197-51] ab259970) at 1/1000 dilution
Lane 1: Rat brain tissue lysate at 20 µg
Lane 2: Rat hippocampus tissue lysate at 20 µg
Lane 3: Rat spleen tissue lysate at 20 µg
Lane 4: Rat skin tissue lysate at 20 µg
Lane 5: Rat thyroid tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 56 kDa
Observed band size: 55 kDa
![Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364), expandable thumbnail](https://content.abcam.com/products/images/vmat2-antibody-epr24197-51-bsa-and-azide-free-ab280364--immunoprecipitation-img138192.jpg "Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)")
Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
This data was developed using Anti-VMAT2 antibody [EPR24197-51] ab259970, the same antibody clone in a different buffer formulation.
VMAT2 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with Anti-VMAT2 antibody [EPR24197-51] ab259970 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-VMAT2 antibody [EPR24197-51] ab259970 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate 10 ug
Lane 2: Anti-VMAT2 antibody [EPR24197-51] ab259970 IP in Mouse brain tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-VMAT2 antibody [EPR24197-51] ab259970 in Mouse brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
Input sample is non-boiled as boiling may cause protein aggregates.
All lanes: Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] (Anti-VMAT2 antibody [EPR24197-51] ab259970)
Predicted band size: 56 kDa
Observed band size: 55 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364), expandable thumbnail](https://content.abcam.com/products/images/vmat2-antibody-epr24197-51-bsa-and-azide-free-ab280364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10422.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
This data was developed using Anti-VMAT2 antibody [EPR24197-51] ab259970, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling VMAT2 with Anti-VMAT2 antibody [EPR24197-51] ab259970 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse cerebrum (PMID: 27836486). The section was incubated with Anti-VMAT2 antibody [EPR24197-51] ab259970 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364), expandable thumbnail](https://content.abcam.com/products/images/vmat2-antibody-epr24197-51-bsa-and-azide-free-ab280364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118616.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
This data was developed using Anti-VMAT2 antibody [EPR24197-51] ab259970, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling VMAT2 with Anti-VMAT2 antibody [EPR24197-51] ab259970 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse cerebrum (PMID: 27836486). The section was incubated with Anti-VMAT2 antibody [EPR24197-51] ab259970 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
![Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364), expandable thumbnail](https://content.abcam.com/products/images/vmat2-antibody-epr24197-51-bsa-and-azide-free-ab280364--immunoprecipitation-img138840.jpg "Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)")
Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
This data was developed using Anti-VMAT2 antibody [EPR24197-51] ab259970, the same antibody clone in a different buffer formulation.
VMAT2 was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 ug with Anti-VMAT2 antibody [EPR24197-51] ab259970 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-VMAT2 antibody [EPR24197-51] ab259970 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Rat brain tissue lysate 10 ug
Lane 2: Anti-VMAT2 antibody [EPR24197-51] ab259970 IP in Rat brain tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-VMAT2 antibody [EPR24197-51] ab259970 in Rat brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
Input sample is non-boiled as boiling may cause protein aggregates.
All lanes: Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] (Anti-VMAT2 antibody [EPR24197-51] ab259970)
Predicted band size: 56 kDa
Observed band size: 55 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364), expandable thumbnail](https://content.abcam.com/products/images/vmat2-antibody-epr24197-51-bsa-and-azide-free-ab280364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img43283.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
This data was developed using Anti-VMAT2 antibody [EPR24197-51] ab259970, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling VMAT2 with Anti-VMAT2 antibody [EPR24197-51] ab259970 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat cerebrum. The section was incubated with Anti-VMAT2 antibody [EPR24197-51] ab259970 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364), expandable thumbnail](https://content.abcam.com/products/images/vmat2-antibody-epr24197-51-bsa-and-azide-free-ab280364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img9008.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
This data was developed using Anti-VMAT2 antibody [EPR24197-51] ab259970, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling VMAT2 with Anti-VMAT2 antibody [EPR24197-51] ab259970 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: almost no staining on mouse spleen.The section was incubated with Anti-VMAT2 antibody [EPR24197-51] ab259970 for 30 minutes at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364), expandable thumbnail](https://content.abcam.com/products/images/vmat2-antibody-epr24197-51-bsa-and-azide-free-ab280364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img40564.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
This data was developed using Anti-VMAT2 antibody [EPR24197-51] ab259970, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse skin tissue labeling VMAT2 with Anti-VMAT2 antibody [EPR24197-51] ab259970 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: almost no staining on mouse skin.The section was incubated with Anti-VMAT2 antibody [EPR24197-51] ab259970 for 30 minutes at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364), expandable thumbnail](https://content.abcam.com/products/images/vmat2-antibody-epr24197-51-bsa-and-azide-free-ab280364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img46647.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
This data was developed using Anti-VMAT2 antibody [EPR24197-51] ab259970, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling VMAT2 with Anti-VMAT2 antibody [EPR24197-51] ab259970 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: almost no staining on rat spleen.The section was incubated with Anti-VMAT2 antibody [EPR24197-51] ab259970 for 30 minutes at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364), expandable thumbnail](https://content.abcam.com/products/images/vmat2-antibody-epr24197-51-bsa-and-azide-free-ab280364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113503.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
This data was developed using Anti-VMAT2 antibody [EPR24197-51] ab259970, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat skin tissue labeling VMAT2 with Anti-VMAT2 antibody [EPR24197-51] ab259970 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: almost no staining on rat skin. The section was incubated with Anti-VMAT2 antibody [EPR24197-51] ab259970 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364), expandable thumbnail](https://content.abcam.com/products/images/vmat2-antibody-epr24197-51-bsa-and-azide-free-ab280364--immunoprecipitation-img138840.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364), expandable thumbnail](https://content.abcam.com/products/images/vmat2-antibody-epr24197-51-bsa-and-azide-free-ab280364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img43283.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)")
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