AB270968

Anti-Von Hippel Lindau/VHL antibody [EPR24354-128]

20ul selling size
RabMAb
Recombinant
What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal Von Hippel Lindau/VHL antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

von Hippel-Lindau disease tumor suppressor, Protein G7, pVHL, VHL

1 Images

Lab

Western blot - Anti-Von Hippel Lindau/VHL antibody [EPR24354-128] (AB270968)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The antibody recognizes both the long and the short isoforms with molecular weights of 24KDa and 19KDa.

Negative control : 786-O (PMID : 9370235)

Exposure time : 48 seconds.

All lanes:

Western blot - Anti-Von Hippel Lindau/VHL antibody [EPR24354-128] (ab270968) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 40 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 3:

CCRF-CEM (human T cell lymphoblast cell line) whole cell lysate at 40 µg

Lane 4:

786-O (human kidney renal cell adenocarcinoma epithelial cell)whole cell lysate at 40 µg

Lane 5:

293T (human embryonic kidney epithelial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 24 kDa

Observed band size: 19 kDa,24 kDa

false

Carrier free

Anti-Von Hippel Lindau/VHL antibody [EPR24354-128] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24354-128

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex (PubMed : 10944113, PubMed : 17981124, PubMed : 19584355). Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions (PubMed : 10944113, PubMed : 17981124). Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases (PubMed : 10944113, PubMed : 17981124). Ubiquitinates, in an oxygen-responsive manner, ADRB2 (PubMed : 19584355). Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR (PubMed : 34290272).

See full target information VHL

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e32889 PubMed39005918

2024

HIF-1α activation impairs dendrites formation and elongation in osteocytogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Li,Jian Zhao,Lei Chen,Xinyi Zhou,Minglong Qiu,Lianfu Deng,Kai Yang,Yaozeng Xu

Clinical and translational medicine 14:e1725 PubMed38886900

2024

Deletion of endothelial IGFBP5 protects against ischaemic hindlimb injury by promoting angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Song,Yu Hu,Yi-Xiang Hong,Hu Sun,Yue Han,Yi-Jie Mao,Wei-Yin Wu,Gang Li,Yan Wang

The Journal of biological chemistry 300:105535 PubMed38072043

2023

FOXA2 activates HIF2α expression to promote tumor progression and is regulated by the E3 ubiquitin ligase VHL in renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dongjun Yang,Qixiang Li,Peifen Lu,Dongliang Wu,Wenyang Li,Xingjun Meng,Mengying Xing,Wenbing Shangguan,Bing Chen,Jie Yang,Zhihong Zhang,Zengjun Wang,David C S Huang,Quan Zhao

iScience 26:107535 PubMed37636080

2023

PROTAC targeting cyclophilin A controls virus-induced cytokine storm.

Applications

Unspecified application

Species

Unspecified reactive species

Heqiao Li,Wenxian Yang,Huizi Li,Xiaoyuan Bai,He Zhang,Wenhui Fan,Wenjun Liu,Lei Sun

Nature communications 14:4274 PubMed37460463

2023

Proteogenomics of clear cell renal cell carcinoma response to tyrosine kinase inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Hailiang Zhang,Lin Bai,Xin-Qiang Wu,Xi Tian,Jinwen Feng,Xiaohui Wu,Guo-Hai Shi,Xiaoru Pei,Jiacheng Lyu,Guojian Yang,Yang Liu,Wenhao Xu,Aihetaimujiang Anwaier,Yu Zhu,Da-Long Cao,Fujiang Xu,Yue Wang,Hua-Lei Gan,Meng-Hong Sun,Jian-Yuan Zhao,Yuanyuan Qu,Dingwei Ye,Chen Ding

Aging and disease 14:529-547 PubMed37008055

2023

Positive Feedback Regulation of Circular RNA Hsa_circ_0000566 and HIF-1α promotes Osteosarcoma Progression and Glycolysis Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Shuying Shen,Yining Xu,Zhe Gong,Teng Yao,Di Qiao,Yizhen Huang,Zhenlei Zhang,Jun Gao,Haonan Ni,Zhanping Jin,Yingchun Zhu,Hongfei Wu,Qingxin Wang,Xiangqian Fang,Kangmao Huang,Jianjun Ma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Von Hippel Lindau/VHL antibody [EPR24354-128]

Western blot - Anti-Von Hippel Lindau/VHL antibody [EPR24354-128] (AB270968)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise