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AB282120

Anti-Von Hippel Lindau/VHL antibody [EPR24354-128] - BSA and Azide free

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Rabbit Recombinant Monoclonal Von Hippel Lindau/VHL antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

von Hippel-Lindau disease tumor suppressor, Protein G7, pVHL, VHL

1 Images
Western blot - Anti-Von Hippel Lindau/VHL antibody [EPR24354-128] - BSA and Azide free (AB282120)
  • WB

Lab

Western blot - Anti-Von Hippel Lindau/VHL antibody [EPR24354-128] - BSA and Azide free (AB282120)

This data was developed using ab270968, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The antibody recognizes both the long and the short isoforms with molecular weights of 24KDa and 19KDa.

Negative control : 786-O (PMID : 9370235)

Exposure time : 48 seconds.

All lanes:

Western blot - Anti-Von Hippel Lindau/VHL antibody [EPR24354-128] (<a href='/en-us/products/primary-antibodies/von-hippel-lindau-vhl-antibody-epr24354-128-ab270968'>ab270968</a>) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 40 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 3:

CCRF-CEM (human T cell lymphoblast cell line) whole cell lysate at 40 µg

Lane 4:

786-O (human kidney renal cell adenocarcinoma epithelial cell)whole cell lysate at 40 µg

Lane 5:

293T (human embryonic kidney epithelial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 24 kDa

Observed band size: 19 kDa,24 kDa

false

  • Unconjugated

    Anti-Von Hippel Lindau/VHL antibody [EPR24354-128]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24354-128

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab282120 is the carrier-free version of ab270968.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex (PubMed : 10944113, PubMed : 17981124, PubMed : 19584355). Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions (PubMed : 10944113, PubMed : 17981124). Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases (PubMed : 10944113, PubMed : 17981124). Ubiquitinates, in an oxygen-responsive manner, ADRB2 (PubMed : 19584355). Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR (PubMed : 34290272).
See full target information VHL
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com