Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (AB273655)

Paraffin-embedded human lung carcinoma tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.

Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (AB273655)

Paraffin-embedded human pancreas tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.

Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (AB273655)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.

Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (AB273655)

Paraffin-embedded human bladder tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.

Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (AB273655)

Paraffin-embedded human colon adenocarcinoma tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.

Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (AB273655)

Paraffin-embedded human ovary adenocarcinoma tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.

Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (AB273655)

Paraffin-embedded human endometrium tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.

Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).

• WB

Supplier Data

Western blot - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (AB273655)

Cells were transfected with cDNA for 48 hours then lysed.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).

All lanes:

Western blot - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655) at 1/4000 dilution

Lane 1:

HEK-293T cells were transfected with the pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cells were transfected with the pCMV6-ENTRY VHL cDNA at 5 µg

Predicted band size: 24 kDa

false