AB194405

Anti-Von Willebrand Factor antibody [3E2D10]

Name: Anti-Von Willebrand Factor antibody [3E2D10] (ab194405) | Abcam
Brand: Abcam Limited
SKU: AB194405
Price: 215 USD
Availability: InStock

Be the first to review this product! Submit a review

(15 Publications)

Mouse Monoclonal Von Willebrand Factor antibody. Suitable for IHC-P and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VWF aa 800-950.

View Alternative Names

F8VWF, VWF, von Willebrand factor, vWF

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [3E2D10] (AB194405)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [3E2D10] (AB194405)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Von Willebrand Factor with ab194405 at 1.0 μg/mL.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [3E2D10] (AB194405)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling Von Willebrand Factor with ab194405 at 2 μg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3E2D10

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human VWF aa 800-950. The exact immunogen used to generate this antibody is proprietary information.

P04275

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>use 10mM Tris with 1mM EDTA, pH 9.0 for antigen retrieval.</p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from Bioreactor Concentrate

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

See full target information VWF

style

left-column

Publications (15)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35955642

2022

Functional Characterization of Human Induced Pluripotent Stem Cell-Derived Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xuehui Fan,Lukas Cyganek,Katja Nitschke,Stefanie Uhlig,Philipp Nuhn,Karen Bieback,Daniel Duerschmied,Ibrahim El-Battrawy,Xiaobo Zhou,Ibrahim Akin

ACS applied materials & interfaces 14:31567-31585 PubMed35815638

2022

Development of an In Vitro Biomimetic Peripheral Neurovascular Platform.

Applications

Unspecified application

Species

Unspecified reactive species

Afonso Malheiro,Adrián Seijas-Gamardo,Abhishek Harichandan,Carlos Mota,Paul Wieringa,Lorenzo Moroni

Journal of oncology 2022:3780854 PubMed35342404

2022

Mechanism of N-Methyl-N-Nitroso-Urea-Induced Gastric Precancerous Lesions in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng-Xiong Zhang,Wen Tian,Yuan-Liang Liu,Jia-Hui Ni,Dan Zhang,Hua-Feng Pan,Zi-Ming Zhao,Bo Ai,Zhe-Sheng Chen,Li-Zhu Lin,Wei Liu

Communications biology 4:1205 PubMed34671074

2021

Mitigating the non-specific uptake of immunomagnetic microparticles enables the extraction of endothelium from human fat.

Applications

Unspecified application

Species

Unspecified reactive species

Jeremy A Antonyshyn,Vienna Mazzoli,Meghan J McFadden,Anthony O Gramolini,Stefan O P Hofer,Craig A Simmons,J Paul Santerre

Frontiers in immunology 12:735922 PubMed34671353

2021

COVID-19 Lung Pathogenesis in SARS-CoV-2 Autopsy Cases.

Applications

Unspecified application

Species

Unspecified reactive species

Silvana Valdebenito,Simon Bessis,Djillali Annane,Geoffroy Lorin de la Grandmaison,Elisabeth Cramer-Bordé,Brendan Prideaux,Eliseo A Eugenin,Morgane Bomsel

Stem cells (Dayton, Ohio) 39:853-865 PubMed33594762

2021

Phage display targeting identifies EYA1 as a regulator of glioblastoma stem cell maintenance and proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

JongMyung Kim,Chunhua She,Marine Potez,Ping Huang,Qiulian Wu,Briana C Prager,Zhixin Qiu,Shideng Bao,Jeremy N Rich,James K C Liu

Frontiers in cellular neuroscience 14:540669 PubMed33584203

2021

Inhibition of Caspase-1 Ameliorates Ischemia-Associated Blood-Brain Barrier Dysfunction and Integrity by Suppressing Pyroptosis Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yubin Liang,Pingping Song,Wei Chen,Xuemin Xie,Rixin Luo,Jiehua Su,Yunhui Zhu,Jiamin Xu,Rongrong Liu,Peizhi Zhu,Yusheng Zhang,Min Huang

Stem cell research & therapy 12:87 PubMed33494812

2021

Exosomes derived from miR-301a-3p-overexpressing adipose-derived mesenchymal stem cells reverse hypoxia-induced erectile dysfunction in rat models.

Applications

Unspecified application

Species

Unspecified reactive species

Li Liang,Dachao Zheng,Chao Lu,Qinghong Xi,Hua Bao,Wengfeng Li,Yufei Gu,Yuanshen Mao,Bin Xu,Xin Gu

Oncology letters 20:303 PubMed33093912

2020

Endothelial transdifferentiation of human HGC-27 gastric cancer cells .

Applications

Unspecified application

Species

Unspecified reactive species

Changxin Chen,Zhixin Hunag,Mucheng Wang,Zicheng Huang,Xiangbo Chen,Anye Huang,Binbin Zheng,Lishan Wu,Yi Liu,Xinwen Wang,Weifeng Xu

Cell death & disease 11:457 PubMed32541667

2020

Snail promotes the generation of vascular endothelium by breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenyu Chang,Yanan Zhang,Jie Liu,Yiqiong Zheng,Huayue Li,Yanjun Kong,Pengyun Li,Haiwen Peng,Yajiao Shi,Bo Cao,Fang Ran,Yingjie Chen,Yuhua Song,Qinong Ye,Lihua Ding

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Von Willebrand Factor antibody [3E2D10]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [3E2D10] (AB194405)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [3E2D10] (AB194405)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (15)

Product promise