AB6994

Anti-Von Willebrand Factor antibody

Name: Von willebrand factor antibody, rabbit polyclonal (ab6994) | Abcam
Brand: Abcam Limited
SKU: AB6994
Price: 650 USD
Availability: InStock
Rating: 4 (61 reviews)

(61 Reviews)

(614 Publications)

Anti-Von Willebrand Factor antibody (ab6994) is a rabbit polyclonal antibody detecting Von Willebrand Factor in Western Blot, Flow Cytometry, IHC-P, IHC-Fr, ICC/IF. Suitable for Cow, Dog, Guinea pig, Horse, Human, Pig, Rat, Sheep.

- Over 500 publications
- Trusted since 2001

View Alternative Names

F8VWF, VWF, von Willebrand factor, vWF

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody (AB6994)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody (AB6994)

Immunohistochemical analysis of Formalin fixed paraffin-embedded sections human kidney tissue labeling Von Willebrand Factor with ab6994 at 1/2000.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody (AB6994)

Immunohistochemical analysis of Formalin fixed paraffin-embedded sections human kidney tissue labeling Von Willebrand Factor with ab6994 at 1/2000.

CiteAb

Immunohistochemistry - Anti-Von Willebrand Factor antibody (AB6994)

Immunohistochemistry using Anti-Von Willebrand Factor antibody, ab6994. Publication image from Huang, S. M. et al., 2013, J Biomed Sci, 23876213. Legend direct from paper.

Engrafted CM-DiI-labeled ASCs were expressed within the endothelial cells. (A-C) Images obtained from 16 µm sections frozen 14 days after the injection of CM-DiI-labeled ASCs (red fluorescence) into tissue with radiation ulcers. Phase contrast (A), red fluorescence (B) and overlay images (C). Bar : 200 µm. (D-K) Confocal images were acquired from 8 µm sections frozen 14 days after the injection of CM-DiI-labeled ASCs and immunostained with vWF (D-G) or CD31 (H-K). Differential interference contrast image (D and H). Red staining indicates CM-DiI-labeled ASCs (E and I). Green staining indicates vWF (F) and CD31 (J). Overlay image (G and K).

CiteAb

Western blot - Anti-Von Willebrand Factor antibody (AB6994)

Von Willebrand Factor western blotting using Anti-Von Willebrand Factor antibody ab6994. Publication image and figure legend from Yang, X., Sun, H. J., et al., 2015, BMC Cancer, PubMed : 25886574.

VEGF treatment induces vWF expression in and secretion from HUVEC cell lines in a dose- and time-dependent manner. HUVECs were exposed to 0, 10, 50 or 100 ng/mL of VEGF for 1 h and examined by western blot (A) and qRT-PCR (C). The concentration of 100 ng/mL induced the highest level of vWF protein and mRNA expression. HUVECs were exposed to the various doses of VEGF for the indicated times and examined by western blot (B) and qRT-PCR (D). The exposure time of 40 minutes stimulated the highest level of vWF protein expression but 80 minutes stimulated the highest level of vWF mRNA expression. *p < 0.05 and **p < 0.01 by Student's t-test.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Sheep, Rat, Pig, Horse, Dog, Guinea pig, Cow

Applications

Flow Cyt, IHC-FoFr, ICC/IF, WB, IHC-FrFl, IHC-P, IHC-Fr

applications

Immunogen

Native Full Length Protein corresponding to Human VWF.

P04275

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Reactivity data

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Perform enzymatic antigen retrieval with 0.1% pronase for 10 min at 35 °C before commencing with IHC protocol.Indirect Immunofluorescence: minimum working dilution of 1:200 was determined using FFPE sections of human tongue with FITC-conjugated secondary.Indirect Immunoperoxidase Labeling: minimum working dilution of 1:800 was determined using FFPE sections of human tongue with biotinylated secondary and signal amplification.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCFrFl-species-checked": "guaranteed", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "(see Abreview)" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCFrFl-species-checked": "guaranteed", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "(see Abreview)" }, "Chicken": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "notRecommended", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "for IF and 1/1000-1/2000 for ABC methods with HRP conjugates. Perform enzymatic antigen retrieval with 0.1% pronase for 10 min at 35 °C before commencing with IHC protocol.Indirect Immunofluorescence: minimum working dilution of 1:200 was determined using FFPE sections of human tongue with FITC-conjugated secondary.Indirect Immunoperoxidase Labeling: minimum working dilution of 1:800 was determined using FFPE sections of human tongue with biotinylated secondary and signal amplification.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCFrFl-species-checked": "notRecommended", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "(see Abreview)" }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCFrFl-species-checked": "guaranteed", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "(see Abreview)" }, "Dog": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCFrFl-species-checked": "guaranteed", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "(see Abreview)" }, "Guinea pig": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCFrFl-species-checked": "guaranteed", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "(see Abreview)" }, "Horse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCFrFl-species-checked": "guaranteed", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "(see Abreview)" }, "Pig": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCFrFl-species-checked": "guaranteed", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "(see Abreview)" }, "Sheep": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCFrFl-species-checked": "guaranteed", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "(see Abreview)" } } }

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Product details

Product Specifications
Anti-Von Willebrand Factor antibody (ab6994) is a rabbit polyclonal antibody and is validated for use in Flow Cyt, ICC/IF, IHC-FoFr, IHC-Fr, IHC-FrFl, IHC-P, WB in cow, dog, guineapig, horse, human, pig, rat, sheep samples.
Anti-Von Willebrand Factor antibody (ab6994) specifically detects Von Willebrand Factor (UniProt ID: P04275; Molecular weight: 81kDa) and is sold in 100 µL selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-Von Willebrand Factor antibody (ab6994) has high sensitivity and specificity.
Anti-Von Willebrand Factor antibody (ab6994) has been cited over 502 times in peer reviewed journals and is trusted by the scientific community.
Anti-Von Willebrand Factor antibody (ab6994) has 61 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other rabbit serum proteins.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

See full target information VWF

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Publications (614)

Recent publications for all applications. Explore the full list and refine your search

Stem cells translational medicine 14: PubMed40973918

2025

Rapid manufacturing of angiogenic cellular collagen patches for ischemic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Eric Pfrender,Sungwoo Kim,John A Farag,Shin Yajima,Yujiro Kawai,Koji Kawago,Umayr Syed,Gentaro Ikeda,Tsuyoshi Ueyama,Hiroyuki Takashima,Alex Dalal,Yuanjia Zhu,Kenzo Ichimura,Yu Liu,Seyedsina Moeinzadeh,Jayme Koltsov,Joseph C Wu,Y Joseph Woo,Phillip C Yang,Yunzhi P Yang,Yasuhiro Shudo

International journal of molecular sciences 26: PubMed40869420

2025

Adaptive and Pathological Changes of the Cardiac Muscle in a Mouse Model of Renocardiac Syndrome: The Role of Nestin-Positive Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Polina A Abramicheva,Ilya A Sokolov,Arina A Druzhinina,Daria M Potashnikova,Nadezda V Andrianova,Dmitry S Semenovich,Vasily N Manskikh,Ljubava D Zorova,Elmira I Yakupova,Ivan M Vikhlyantsev,Olga S Tarasova,Dmitry B Zorov,Egor Y Plotnikov

Plastic and reconstructive surgery. Global open 13:e7062 PubMed40771260

2025

Efficacy of Combined Use of PELNAC and Platelet-rich Plasma in Promoting Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroshi Fujimaki,Hajime Matsumine,Yuki Hasegawa,Yosuke Niimi,Hiroyuki Sakurai

Communications materials 6:151 PubMed40688364

2025

Evaluation of in situ tissue-engineered arteriovenous grafts suitable for cannulation in a large animal model.

Applications

Unspecified application

Species

Unspecified reactive species

Paul J Besseling,Wojciech Szymczyk,Martin Teraa,Raechel J Toorop,Paul A A Bartels,Boris Arts,Rob C H Driessen,Arturo M Lichauco,Hidde C Bakker,Joost O Fledderus,Gert J de Borst,Patricia Y W Dankers,Carlijn V C Bouten,Marianne C Verhaar

PloS one 20:e0326138 PubMed40549708

2025

The bovine oviductal environment and composition are negatively affected by elevated body energy reserves.

Applications

Unspecified application

Species

Unspecified reactive species

Natália Marins Bastos,Rodrigo Silva Goulart,Alessandra Bridi,Rosane Mazzarella,Luana Alves,Paola Maria da Silva Rosa,Ricardo de Francisco Strefezzi,Lindsay Baltel Paskoski,Ricardo Perecin Nociti,Juliano Rodrigues Sangalli,Schaienni Fontoura Saldanha,Camila Azzolin de Souza,Angélica Camargo Dos Santos,Marcos Roberto Chiaratti,Guilherme Pugliesi,Flávio Vieira Meirelles,Felipe Perecin,Juliano Coelho da Silveira

Journal of clinical medicine 14: PubMed40507663

2025

Short-Term Administration of Parathyroid Hormone Improves Wound Healing Around Implants in an Osteoporotic Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Farah A Al-Omari,Shinichiro Kuroshima,Ryohei Kozutsumi,Takashi Sawase

Interdisciplinary cardiovascular and thoracic surgery 40: PubMed40465413

2025

A novel decellularization protocol with cryopreservation of pulmonary allografts in an ovine model.

Applications

Unspecified application

Species

Unspecified reactive species

Lucas Van Hoof,Geoffroy de Beco,Manon Van Hecke,Piet Claus,Alexander Van De Bruaene,Tania Roskams,Cristina Castells-Sala,José Rodríguez Martínez,Ana Vilarrodona,Stefano Mastrobuoni,Ramadan Jashari,Filip Rega

Frontiers in immunology 16:1581599 PubMed40356930

2025

Donor adipose-derived stromal cells are vasoprotectant but unable to revert acute rejection in rodent vascularized composite allotransplants.

Applications

Unspecified application

Species

Unspecified reactive species

Riccardo Schweizer,Pranitha Kamat,Holger J Klein,Branislav Kollar,Matthias Waldner,Klara Stölzl,Fabienne Lehner,Souzan Salemi,Peter Bode,Daniel Eberli,Adriano Taddeo,Jan A Plock

Burns & trauma 13:tkaf005 PubMed40321300

2025

A bioactive hydrogel patch accelerates revascularization in ischemic lesions for tissue repair.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuo Liu,Kang Wu,Hong Zeng,Wenxin Huang,Xuemeng Wang,Ying Qu,Chuntao Chen,Lei Zhang,Dongpin Sun,Sifeng Chen,Xiao Lin,Ning Sun,Lei Yang,Chen Xu

Cell transplantation 34:9636897251328419 PubMed40264358

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A novel method of pancreatic islet transplantation at the liver surface using a gelatin hydrogel nonwoven fabric.

Applications

Unspecified application

Species

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Yukiko Endo Kumata,Akiko Inagaki,Yasuhiro Nakamura,Takehiro Imura,Ryusuke Saito,Takumi Katano,Shoki Suzuki,Kazuaki Tokodai,Takashi Kamei,Michiaki Unno,Kimiko Watanabe,Yasuhiko Tabata,Masafumi Goto

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Von Willebrand Factor antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody (AB6994)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody (AB6994)

Immunohistochemistry - Anti-Von Willebrand Factor antibody (AB6994)

Western blot - Anti-Von Willebrand Factor antibody (AB6994)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (614)

Product promise