Anti-Von Willebrand Factor antibody [EPR12011] - BSA and Azide free

6 product images

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  Western blot - Anti-Von Willebrand Factor antibody [EPR12011] - BSA and Azide free (ab240177)

  This data was developed using Anti-Von Willebrand Factor antibody [EPR12011] ab174290, the same antibody clone in a different buffer formulation.
  

  We are unsure how to define the extra bands.

  All lanes: Western blot - Anti-Von Willebrand Factor antibody [EPR12011] (Anti-Von Willebrand Factor antibody [EPR12011] ab174290) at 1/1000 dilution

  Lane 1: Human serum lysates at 20 µg

  Lane 2: Human plasma lysates at 20 µg

  Lane 3: Rat plasma lysates at 20 µg

  Lane 4: Mouse platelet lysates at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Predicted band size: 309 kDa

  Observed band size: 309 kDa

• 

  Western blot - Anti-Von Willebrand Factor antibody [EPR12011] - BSA and Azide free (ab240177)

  This data was developed using Anti-Von Willebrand Factor antibody [EPR12011] ab174290, the same antibody clone in a different buffer formulation.

  All lanes: Western blot - Anti-Von Willebrand Factor antibody [EPR12011] (Anti-Von Willebrand Factor antibody [EPR12011] ab174290) at 1/1000 dilution

  Lane 1: Human serum lysate at 10 µg

  Lane 2: Human plasma lysate at 10 µg

  Predicted band size: 309 kDa

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [EPR12011] - BSA and Azide free (ab240177)

  Immunohistochemical analysis of paraffin-embedded human esophagus labeling Von Willebrand Factor with Anti-Von Willebrand Factor antibody [EPR12011] ab174290 at 1/10000 dilution, incubated with Anti-Von Willebrand Factor antibody [EPR12011] ab174290 for 30 mins at room temperature; followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on the vessels in human esophagus. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
  This data was developed using Anti-Von Willebrand Factor antibody [EPR12011] ab174290, the same antibody clone in a different buffer formulation.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [EPR12011] - BSA and Azide free (ab240177)

  Immunohistochemical analysis of paraffin-embedded rat spleen labeling Von Willebrand Factor with Anti-Von Willebrand Factor antibody [EPR12011] ab174290 at 1/10000 dilution, incubated with Anti-Von Willebrand Factor antibody [EPR12011] ab174290 for 30 mins at room temperature; followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on the vessels in rat spleen. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
  This data was developed using Anti-Von Willebrand Factor antibody [EPR12011] ab174290, the same antibody clone in a different buffer formulation.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [EPR12011] - BSA and Azide free (ab240177)

  Immunohistochemical analysis of paraffin-embedded mouse spleen labeling Von Willebrand Factor with Anti-Von Willebrand Factor antibody [EPR12011] ab174290 at 1/10000 dilution, incubated with Anti-Von Willebrand Factor antibody [EPR12011] ab174290 for 30 mins at room temperature; followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on the vessels in mouse spleen. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
  This data was developed using Anti-Von Willebrand Factor antibody [EPR12011] ab174290, the same antibody clone in a different buffer formulation.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [EPR12011] - BSA and Azide free (ab240177)

  Immunohistochemical analysis of paraffin-embedded human tonsil labeling Von Willebrand Factor with Anti-Von Willebrand Factor antibody [EPR12011] ab174290 at 1/10000 dilution, incubated with Anti-Von Willebrand Factor antibody [EPR12011] ab174290 for 30 mins at room temperature; followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on the vessels in human tonsil. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
  This data was developed using Anti-Von Willebrand Factor antibody [EPR12011] ab174290, the same antibody clone in a different buffer formulation.