Anti-Von Willebrand Factor antibody [EPR12011] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [EPR12011] - BSA and Azide free (AB240177)

Immunohistochemical analysis of paraffin-embedded human esophagus labeling Von Willebrand Factor with ab174290 at 1/10000 dilution, incubated with ab174290 for 30 mins at room temperature; followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on the vessels in human esophagus. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. This data was developed using ab174290, the same antibody clone in a different buffer formulation.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [EPR12011] - BSA and Azide free (AB240177)

Immunohistochemical analysis of paraffin-embedded human tonsil labeling Von Willebrand Factor with ab174290 at 1/10000 dilution, incubated with ab174290 for 30 mins at room temperature; followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on the vessels in human tonsil. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. This data was developed using ab174290, the same antibody clone in a different buffer formulation.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [EPR12011] - BSA and Azide free (AB240177)

Immunohistochemical analysis of paraffin-embedded mouse spleen labeling Von Willebrand Factor with ab174290 at 1/10000 dilution, incubated with ab174290 for 30 mins at room temperature; followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on the vessels in mouse spleen. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. This data was developed using ab174290, the same antibody clone in a different buffer formulation.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [EPR12011] - BSA and Azide free (AB240177)

Immunohistochemical analysis of paraffin-embedded rat spleen labeling Von Willebrand Factor with ab174290 at 1/10000 dilution, incubated with ab174290 for 30 mins at room temperature; followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on the vessels in rat spleen. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. This data was developed using ab174290, the same antibody clone in a different buffer formulation.

• WB

Supplier Data

Western blot - Anti-Von Willebrand Factor antibody [EPR12011] - BSA and Azide free (AB240177)

This data was developed using ab174290, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Von Willebrand Factor antibody [EPR12011] (<a href='/en-us/products/primary-antibodies/von-willebrand-factor-antibody-epr12011-ab174290'>ab174290</a>) at 1/1000 dilution

Lane 1:

Human serum lysate at 10 µg

Lane 2:

Human plasma lysate at 10 µg

Predicted band size: 309 kDa

false

• WB

Lab

Western blot - Anti-Von Willebrand Factor antibody [EPR12011] - BSA and Azide free (AB240177)

This data was developed using ab174290, the same antibody clone in a different buffer formulation.

We are unsure how to define the extra bands.

All lanes:

Western blot - Anti-Von Willebrand Factor antibody [EPR12011] (<a href='/en-us/products/primary-antibodies/von-willebrand-factor-antibody-epr12011-ab174290'>ab174290</a>) at 1/1000 dilution

Lane 1:

Human serum lysates at 20 µg

Lane 2:

Human plasma lysates at 20 µg

Lane 3:

Rat plasma lysates at 20 µg

Lane 4:

Mouse platelet lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 309 kDa

Observed band size: 309 kDa

false