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Rabbit Recombinant Monoclonal Von Willebrand Factor antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples.

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Images

Western blot - Anti-Von Willebrand Factor antibody [EPR18569-105] - BSA and Azide free (AB228523), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested

Tested
Tested

Species

Mouse, Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

Alternative names

von Willebrand factor, vWF, F8VWF, VWF

Recommended products

Rabbit Recombinant Monoclonal Von Willebrand Factor antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples.

Alternative names

von Willebrand factor, vWF, F8VWF, VWF

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR18569-105

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab228523 is the carrier-free version of Anti-Von Willebrand Factor antibody [EPR18569-105] ab189500.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-Von Willebrand Factor antibody [EPR18569-105] - BSA and Azide free (ab228523), expandable thumbnail

    Western blot - Anti-Von Willebrand Factor antibody [EPR18569-105] - BSA and Azide free (ab228523)

    Exposure times: Lanes 1,2: 3 minutes; Lane 3: 1 minute; Lane 4,5: 15 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular masses are consistent with what has been described in the literature (PMID: 8204880; PMID: 18344423).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Von Willebrand Factor antibody [EPR18569-105] ab189500).

    All lanes: Western blot - Anti-Von Willebrand Factor antibody [EPR18569-105] (Anti-Von Willebrand Factor antibody [EPR18569-105] ab189500) at 1/1000 dilution

    Lane 1: Human liver lysate at 20 µg

    Lane 2: Human plasma at 20 µg

    Lane 3: Hep1-6 (mouse hepatoma epithelial cell line) whole cell lysate at 20 µg

    Lane 4: Mouse plasma at 20 µg

    Lane 5: Mouse liver lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 309 kDa

    Observed band size: 309 kDa, 90 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com