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AB250539

Anti-Von Willebrand Factor antibody [EPR2992(N)] - BSA and Azide free

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Rabbit Recombinant Monoclonal Von Willebrand Factor antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

F8VWF, VWF, von Willebrand factor, vWF

2 Images
Western blot - Anti-Von Willebrand Factor antibody [EPR2992(N)] - BSA and Azide free (AB250539)
  • WB

Supplier Data

Western blot - Anti-Von Willebrand Factor antibody [EPR2992(N)] - BSA and Azide free (AB250539)

This data was developed using ab181871, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Von Willebrand Factor antibody [EPR2992(N)] (<a href='/en-us/products/primary-antibodies/von-willebrand-factor-antibody-epr2992n-ab181871'>ab181871</a>) at 1/2000 dilution

Lane 1:

Human plasma lysate at 20 µg

Lane 2:

Human serum lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 309 kDa

Observed band size: 309 kDa

false

Immunoprecipitation - Anti-Von Willebrand Factor antibody [EPR2992(N)] - BSA and Azide free (AB250539)
  • IP

Supplier Data

Immunoprecipitation - Anti-Von Willebrand Factor antibody [EPR2992(N)] - BSA and Azide free (AB250539)

This data was developed using ab181871, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from Human plasma, labeling Von Willebrand Factor immunoprecipitated using ab181871 at 1/70 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-Von Willebrand Factor antibody [EPR2992(N)] (<a href='/en-us/products/primary-antibodies/von-willebrand-factor-antibody-epr2992n-ab181871'>ab181871</a>)

Predicted band size: 309 kDa

false

  • Unconjugated

    Anti-Von Willebrand Factor antibody [EPR2992(N)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2992(N)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250539 is the carrier-free version of ab181871.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the gene VWF is important for maintaining hemostasis. It promotes platelet adhesion at sites of vascular injury by forming a molecular bridge between the sub-endothelial collagen matrix and the platelet-surface receptor complex GPIb-IX-V. Additionally, it acts as a chaperone for coagulation factor VIII, delivering it to the injury site, stabilizing its heterodimeric structure, and protecting it from premature clearance from plasma. This supplementary information is collated from multiple sources and compiled automatically.
See full target information VWF
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com