AB154193

Anti-Von Willebrand Factor antibody [EPSISR15]

RabMAb
Recombinant
What is this?

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(15 Publications)

Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193) is a rabbit monoclonal antibody detecting Von Willebrand Factor in Western Blot, Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

F8VWF, VWF, von Willebrand factor, vWF

6 Images

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Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

All lanes:

Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193) at 1/1000 dilution

Lane 1:

Human serum at 10 µg

Lane 2:

Human plasma at 10 µg

Predicted band size: 309 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Immunofluorescent staining of HepG2 cells labeling Von-Willebrand-Factor with unpurified ab154193 at 1/250 dilution.

Flow Cytometry (Intracellular) - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Von Willebrand Factor (red) with purified ab154193 at a 1/130 dilution (10ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Von Willebrand Factor with Purified ab154193 at 1 : 250 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1 : 200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Lab

Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Blocking and diluting buffer : 5% NFDM/TBST

The expression profile observed is consistent with the literature (PMID : 24769235) in which ab154193 was validated by knockdown assay in HepG2 cell line.

All lanes:

Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193) at 4 µg/mL

All lanes:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution

Predicted band size: 309 kDa

false

Exposure time: 30s

Lab

Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193) at 1/1000 dilution

All lanes:

Human plasma lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 221 kDa,309 kDa

Observed band size: 221 kDa,309 kDa

false

HRP

HRP Anti-Von Willebrand Factor antibody [EPSISR15]
Carrier free

Anti-Von Willebrand Factor antibody [EPSISR15] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Von Willebrand Factor antibody [EPSISR15]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Von Willebrand Factor antibody [EPSISR15]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Von Willebrand Factor antibody [EPSISR15]
660 APC

APC Anti-Von Willebrand Factor antibody [EPSISR15]
578 PE

PE Anti-Von Willebrand Factor antibody [EPSISR15]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Von Willebrand Factor antibody [EPSISR15]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPSISR15

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200", "FlowCytIntra-species-notes": "" } } }

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You may be interested in:

AB108918

Human Von Willebrand Factor ELISA Kit (VWF)

0 Reviews

View product

Why is this recommended?

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

What is this antibody validated in?
Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of Von Willebrand Factor?
Anti-Von Willebrand Factor [EPSISR15] (ab154193) specifically detects a band for Von Willebrand Factor (UniProt: P04275) at a molecular weight of 309kDa.

Trusted by the scientific community
Anti-Von Willebrand Factor [EPSISR15] (ab154193) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPSISR15] also available for your convenience: ab154193, Alexa Fluor® 488 - ab195028, Alexa Fluor® 647 - ab195029, HRP - ab195030, Carrier free - ab232473, APC - ab310828, PE - ab310904, Alexa Fluor® 594 - ab311713, Alexa Fluor® 568 - ab312989, Alexa Fluor® 555 - ab313194

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the gene VWF is important for maintaining hemostasis. It promotes platelet adhesion at sites of vascular injury by forming a molecular bridge between the sub-endothelial collagen matrix and the platelet-surface receptor complex GPIb-IX-V. Additionally, it acts as a chaperone for coagulation factor VIII, delivering it to the injury site, stabilizing its heterodimeric structure, and protecting it from premature clearance from plasma. This supplementary information is collated from multiple sources and compiled automatically.

See full target information VWF

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37686378

2023

Enhanced Angiogenesis in HUVECs Preconditioned with Media from Adipocytes Differentiated from Lipedema Adipose Stem Cells In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Al-Ghadban,Samantha G Walczak,Spencer U Isern,Elizabeth C Martin,Karen L Herbst,Bruce A Bunnell

Cell communication and signaling : CCS 21:176 PubMed37480055

2023

Neutrophil extracellular traps promote angiogenesis in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shifeng Yang,Boshi Sun,Jiacheng Li,Nana Li,Ange Zhang,Xinyu Zhang,Hao Yang,Xiaoming Zou

Journal of tissue engineering 13:20417314221143240 PubMed36600998

2022

Microvessels derived from hiPSCs are a novel source for angiogenesis and tissue regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Gao,Shixing Ma,Xiaotao Xing,Jian Yang,Xun Xu,Cheng Liang,Yejia Yu,Lei Liu,Li Liao,Weidong Tian

Frontiers in endocrinology 13:830466 PubMed35399949

2022

Integration of Metabolomics and Proteomics in Exploring the Endothelial Dysfunction Mechanism Induced by Serum Exosomes From Diabetic Retinopathy and Diabetic Nephropathy Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yang,Dongwei Liu,Zhangsuo Liu

Experimental and therapeutic medicine 22:1441 PubMed34721683

2021

Semaphorin 7A knockdown improves injury and prevents endothelial-to-mesenchymal transition in ox-LDL-induced HUVECs by regulating β1 integrin expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoying Song,Jing Meng,Guoliang Yan,Haihui Wang,Haitao Li,Danfei Lou

Experimental and therapeutic medicine 22:1415 PubMed34676008

2021

Adipose mesenchymal stem cells and gingival mesenchymal stem cells have a comparable effect in endothelium repair.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Yang,Dongmei Xie,Wanwen Lin,Peng Xiang,Chaoquan Peng

Frontiers in bioengineering and biotechnology 9:761861 PubMed34660561

2021

Coaxially Bioprinted Cell-Laden Tubular-Like Structure for Studying Glioma Angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanzhi Wang,Xinda Li,Yi Zhang,Xiaoyan Long,Haitao Zhang,Tao Xu,Chaoshi Niu

Oxidative medicine and cellular longevity 2021:9993240 PubMed34394840

2021

Recombinant High-Mobility Group Box 1 (rHMGB1) Promotes NRF2-Independent Mitochondrial Fusion through CXCR4/PSMB5-Mediated Drp1 Degradation in Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shunrong Zhang,Fei Feng,Jingting Dai,Jia Li,Xiangye Bu,Xiaojie Xie

Journal of immunology research 2021:8100298 PubMed34337080

2021

Exosomes Derived from Nerve Stem Cells Loaded with FTY720 Promote the Recovery after Spinal Cord Injury in Rats by PTEN/AKT Signal Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianbin Chen,Can Zhang,Shouye Li,Zheming Li,Xiaojing Lai,Qingqing Xia

Cell biology international 44:2588-2597 PubMed32808723

2020

An effective method of isolating microvascular endothelial cells from the human dermis.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Hou,Jiao Li,Ling Zhou,Jiannan Liang,Juanjuan Wang,Junqin Li,Ruixia Hou,Juan Li,Xiaohong Yang,Kaiming Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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primary-antibodies

von-willebrand-factor-antibody-vwf-1465-ab218333

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Von Willebrand Factor antibody [EPSISR15]

Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Flow Cytometry (Intracellular) - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Related conjugates and formulations (9)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

You may be interested in:

Human Von Willebrand Factor ELISA Kit (VWF)

Why is this recommended?

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (15)

Product promise

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