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Rabbit Recombinant Monoclonal Von Willebrand Factor antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 15 publications.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193), expandable thumbnail
  • Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193), expandable thumbnail
  • Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193), expandable thumbnail
  • Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/250 - 1/500

Notes

-

Tested
Tested

Species

Human

Dilution info

1/200

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

Alternative names

von Willebrand factor, vWF, F8VWF, VWF

Recommended products

Rabbit Recombinant Monoclonal Von Willebrand Factor antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 15 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPSISR15

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193)

    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Von Willebrand Factor with Purified ab154193 at 1:250 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193), expandable thumbnail

    Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193)

    Blocking and diluting buffer: 5% NFDM/TBST

    The expression profile observed is consistent with the literature (PMID:24769235) in which ab154193 was validated by knockdown assay in HepG2 cell line.

    All lanes: Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193) at 4 µg/mL

    All lanes: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution

    Predicted band size: 309 kDa

    Exposure time: 30s

  • Flow Cytometry (Intracellular) - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193)

    Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Von Willebrand Factor (red) with purified ab154193 at a 1/130 dilution (10ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193), expandable thumbnail

    Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193)

    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193) at 1/1000 dilution

    All lanes: Human plasma lysates at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 221 kDa, 309 kDa

    Observed band size: 221 kDa, 309 kDa

  • Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193), expandable thumbnail

    Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193)

    All lanes: Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193) at 1/1000 dilution

    Lane 1: Human serum at 10 µg

    Lane 2: Human plasma at 10 µg

    Predicted band size: 309 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193)

    Immunofluorescent staining of HepG2 cells labeling Von-Willebrand-Factor with unpurified ab154193 at 1/250 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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