Anti-Von Willebrand Factor antibody [RM1068] (ab316979)

Rabbit Recombinant Multiclonal Von Willebrand Factor antibody. Suitable for WB, IHC-P, IP, IHC-Fr, Flow Cyt and reacts with Human, Mouse, Rat samples.

Images

Western blot - Anti-Von Willebrand Factor antibody [RM1068] (AB316979), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Multiclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	IP	IHC-Fr	Flow Cyt	ICC/IF
Human	Tested	Tested	Not recommended	Expected	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Tested	Tested	Not recommended
Rat	Tested	Tested	Tested	Tested	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -
Species Rat	Dilution info 1/30	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -
Species Rat	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Target data

See full target information VWF

Function

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

Alternative names

F8VWF, VWF, von Willebrand factor, vWF

Recommended products

Rabbit Recombinant Multiclonal Von Willebrand Factor antibody. Suitable for WB, IHC-P, IP, IHC-Fr, Flow Cyt and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Multiclonal
Immunogens: The exact immunogen used to generate this antibody is proprietary information.
Clone number: RM1068
Purification technique: Affinity purification Protein A
Specificity: Unsuitable for human IP, human and rat FC.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

Western blot - Anti-Von Willebrand Factor antibody [RM1068] (ab316979)
Von Willebrand Factor Western blot staining using rabbit Anti-Von Willebrand Factor antibody
Negative controls: HeLa and HT-29.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-Von Willebrand Factor antibody [RM1068] (ab316979) at 1/1000 dilution
Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 40 µg with 5% NFDM/TBST
Lane 2: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 40 µg with 5% NFDM/TBST
Lane 3: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 40 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 300 kDa, 309 kDa, 124 kDa
Exposure time: 180s
Flow Cytometry - Anti-Von Willebrand Factor antibody [RM1068] (ab316979)
Flow cytometric analysis of Mouse PBMC cells labelling Von Willebrand Factor with ab316979 at 1/500 dilution (0.1ug)/right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Cells were co-stained with anti-CD41 conjugated to APC.

Gated on viable cells.
Western blot - Anti-Von Willebrand Factor antibody [RM1068] (ab316979)
The bands beneath the target band (300 kDa) are expected to be cleavage fragments (PMID: 21909423).
Exposure time: Lanes 1-2: 37 seconds; Lanes 3-4: 180 seconds
All lanes: Western blot - Anti-Von Willebrand Factor antibody [RM1068] (ab316979) at 1/1000 dilution
Lane 1: Mouse platelet lysate at 2 µg with 5% NFDM/TBST
Lane 2: Rat platelet lysate at 2 µg with 5% NFDM/TBST
Lane 3: Human plasma lysate at 40 µg with 5% NFDM/TBST
Lane 4: Human serum lysate at 40 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 300 kDa
Western blot - Anti-Von Willebrand Factor antibody [RM1068] (ab316979)
The bands beneath the target band (300 kDa) are expected to be cleavage fragments (PMID: 21909423).
Exposure time: Lane 1: 136 seconds; Lane 2: 180 seconds
All lanes: Western blot - Anti-Von Willebrand Factor antibody [RM1068] (ab316979) at 1/1000 dilution
Lane 1: Mouse lung tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Rat lung tissue lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 300 kDa
Immunohistochemistry (Frozen sections) - Anti-Von Willebrand Factor antibody [RM1068] (ab316979)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat lung (fresh frozen) tissue labeling Von Willebrand Factor with ab316979 at 1/500 (0.958 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing positive staining in endothelial cells of rat lung. The nuclear counterstain was DAPI (Blue). The section was incubated with ab316979 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.
Immunohistochemistry (Frozen sections) - Anti-Von Willebrand Factor antibody [RM1068] (ab316979)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung (fresh frozen) tissue labeling Von Willebrand Factor with ab316979 at 1/500 (0.958 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing positive staining in endothelial cells of mouse lung. The nuclear counterstain was DAPI (Blue). The section was incubated with ab316979 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [RM1068] (ab316979)
Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling Von Willebrand Factor with ab316979 at 1/2000 (0.24 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on endothelial cells in rat lung. The section was incubated with ab316979 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [RM1068] (ab316979)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Von Willebrand Factor with ab316979 at 1/2000 (0.24 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on platelets and megakaryocytes in rat spleen. The section was incubated with ab316979 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [RM1068] (ab316979)
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Von Willebrand Factor with ab316979 at 1/2000 (0.24 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on endothelial cells in mouse lung. The section was incubated with ab316979 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [RM1068] (ab316979)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Von Willebrand Factor with ab316979 at 1/2000 (0.24 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on platelets and megakaryocytes in mouse spleen. The section was incubated with ab316979 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [RM1068] (ab316979)
Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling Von Willebrand Factor with ab316979 at 1/2000 (0.24 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on endothelial cells in human prostatic hyperplasia. The section was incubated with ab316979 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [RM1068] (ab316979)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Von Willebrand Factor with ab316979 at 1/2000 (0.24 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on endothelial cells in human tonsil. The section was incubated with ab316979 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunoprecipitation - Anti-Von Willebrand Factor antibody [RM1068] (ab316979)
Von Willebrand Factor was immunoprecipitated from 0.35 mg Rat platelet lysate with ab316979 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316979 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Rat platelet lysate

Lane 2: ab316979 IP in Rat platelet lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab316979 in rat platelet lysate.
All lanes: Immunoprecipitation - Anti-Von Willebrand Factor antibody [RM1068] (ab316979) at 1/30 dilution
All lanes: Rat platelet lysate with 5% NFDM/TBST
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 24s
Immunoprecipitation - Anti-Von Willebrand Factor antibody [RM1068] (ab316979)
Von Willebrand Factor was immunoprecipitated from 0.35 mg Mouse platelet lysate with ab316979 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316979 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse platelet lysate

Lane 2: ab316979 IP in Mouse platelet lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab316979 in mouse platelet lysate.
The bands beneath the target band (300 kDa) are expected to be cleavage fragments (PMID: 21909423).
All lanes: Immunoprecipitation - Anti-Von Willebrand Factor antibody [RM1068] (ab316979) at 1/30 dilution
All lanes: Mouse platelet lysate with 5% NFDM/TBST
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 50s