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AB316980

Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free

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Rabbit Recombinant Multiclonal Von Willebrand Factor antibody. Carrier free. Suitable for WB, IHC-P, IP, IHC-Fr, Flow Cyt and reacts with Human, Mouse, Rat samples.

View Alternative Names

F8VWF, VWF, von Willebrand factor, vWF

14 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)

This data was developed using ab316979, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Von Willebrand Factor with ab316979 at 1/2000 (0.24 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on endothelial cells in human tonsil. The section was incubated with ab316979 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)

This data was developed using ab316979, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling Von Willebrand Factor with ab316979 at 1/2000 (0.24 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on endothelial cells in human prostatic hyperplasia. The section was incubated with ab316979 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

Flow Cytometry - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)

This data was developed using ab316979, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse PBMC cells labelling Von Willebrand Factor with ab316979 at 1/500 dilution (0.1ug)/right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti-CD41 conjugated to APC.
Gated on viable cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)

This data was developed using ab316979, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Von Willebrand Factor with ab316979 at 1/2000 (0.24 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on platelets and megakaryocytes in rat spleen. The section was incubated with ab316979 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)

This data was developed using ab316979, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling Von Willebrand Factor with ab316979 at 1/2000 (0.24 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on endothelial cells in rat lung. The section was incubated with ab316979 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)

This data was developed using ab316979, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Von Willebrand Factor with ab316979 at 1/2000 (0.24 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on platelets and megakaryocytes in mouse spleen. The section was incubated with ab316979 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)

This data was developed using ab316979, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat lung (fresh frozen) tissue labeling Von Willebrand Factor with ab316979 at 1/500 (0.958 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

Confocal image showing positive staining in endothelial cells of rat lung. The nuclear counterstain was DAPI (Blue). The section was incubated with ab316979 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

Immunohistochemistry (Frozen sections) - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)

This data was developed using ab316979, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung (fresh frozen) tissue labeling Von Willebrand Factor with ab316979 at 1/500 (0.958 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

Confocal image showing positive staining in endothelial cells of mouse lung. The nuclear counterstain was DAPI (Blue). The section was incubated with ab316979 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)

This data was developed using ab316979, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Von Willebrand Factor with ab316979 at 1/2000 (0.24 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on endothelial cells in mouse lung. The section was incubated with ab316979 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

Immunoprecipitation - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)
  • IP

Supplier Data

Immunoprecipitation - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)

This data was developed using ab316979, the same antibody clone in a different buffer formulation.

Von Willebrand Factor was immunoprecipitated from 0.35 mg Rat platelet lysate with ab316979 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316979 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat platelet lysate
Lane 2 : ab316979 IP in Rat platelet lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab316979 in rat platelet lysate.

All lanes:

Immunoprecipitation - Anti-Von Willebrand Factor antibody [RM1068] (<a href='/en-us/products/primary-antibodies/von-willebrand-factor-antibody-rm1068-ab316979'>ab316979</a>) at 1/30 dilution

All lanes:

Rat platelet lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 24s

Immunoprecipitation - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)
  • IP

Supplier Data

Immunoprecipitation - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)

This data was developed using ab316979, the same antibody clone in a different buffer formulation.

Von Willebrand Factor was immunoprecipitated from 0.35 mg Mouse platelet lysate with ab316979 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316979 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse platelet lysate
Lane 2 : ab316979 IP in Mouse platelet lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab316979 in mouse platelet lysate.

The bands beneath the target band (300 kDa) are expected to be cleavage fragments (PMID : 21909423).

All lanes:

Immunoprecipitation - Anti-Von Willebrand Factor antibody [RM1068] (<a href='/en-us/products/primary-antibodies/von-willebrand-factor-antibody-rm1068-ab316979'>ab316979</a>) at 1/30 dilution

All lanes:

Mouse platelet lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 50s

Western blot - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)
  • WB

Supplier Data

Western blot - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)

This data was developed using ab316979, the same antibody clone in a different buffer formulation.

The bands beneath the target band (300 kDa) are expected to be cleavage fragments (PMID : 21909423).

Exposure time : Lanes 1-2 : 37 seconds; Lanes 3-4 : 180 seconds

All lanes:

Western blot - Anti-Von Willebrand Factor antibody [RM1068] (<a href='/en-us/products/primary-antibodies/von-willebrand-factor-antibody-rm1068-ab316979'>ab316979</a>) at 1/1000 dilution

Lane 1:

Mouse platelet lysate at 2 µg

Lane 2:

Rat platelet lysate at 2 µg

Lane 3:

Human plasma lysate at 40 µg

Lane 4:

Human serum lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 300 kDa

false

Western blot - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)
  • WB

Supplier Data

Western blot - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)

This data was developed using ab316979, the same antibody clone in a different buffer formulation.

Negative controls : HeLa and HT-29.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Von Willebrand Factor antibody [RM1068] (<a href='/en-us/products/primary-antibodies/von-willebrand-factor-antibody-rm1068-ab316979'>ab316979</a>) at 1/1000 dilution

Lane 1:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 40 µg

Lane 2:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 3:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 300 kDa,309 kDa,124 kDa

false

Exposure time: 180s

Western blot - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)
  • WB

Supplier Data

Western blot - Anti-Von Willebrand Factor antibody [RM1068] - BSA and Azide free (AB316980)

This data was developed using ab316979, the same antibody clone in a different buffer formulation.

The bands beneath the target band (300 kDa) are expected to be cleavage fragments (PMID : 21909423).

Exposure time : Lane 1 : 136 seconds; Lane 2 : 180 seconds

All lanes:

Western blot - Anti-Von Willebrand Factor antibody [RM1068] (<a href='/en-us/products/primary-antibodies/von-willebrand-factor-antibody-rm1068-ab316979'>ab316979</a>) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 20 µg

Lane 2:

Rat lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 300 kDa

false

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RM1068

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

Flow Cyt, IHC-P, WB, IP, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Unsuitable for human IP, human and rat FC.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab316980 is the carrirer-free version of ab316979.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
See full target information VWF
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com