AB4808

Anti-VP16 tag antibody

Name: Anti-VP16 tag antibody. Rabbit polyclonal (ab4808) | Abcam
Brand: Abcam Limited
SKU: AB4808
Price: 555 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(27 Publications)

Rabbit Polyclonal VP16 tag antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Tag samples. Cited in 27 publications.

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VP16 tag antibody (AB4808)

IHC-P

AbReview4297****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VP16 tag antibody (AB4808)

ab4808 at a 1/100 dilution staining mouse brain sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval, the antibody was incubated with the tissue for 24 hours. Bound antibody was detected using an Alexa-Fluor 488® conjugated Donkey anti-Rabbit IgG.

This image is courtesy of an Abreview submitted on 10 March 2006.

Project260****

Western blot - Anti-VP16 tag antibody (AB4808)

Lane 1:

Marker

Lanes 2 - 3:

Western blot - Anti-VP16 tag antibody (ab4808) at 1 µg/mL

Lane 2:

GAL4-VP16 human recombinant E.coli

Lane 3:

GAL4-VP16 human recombinant E.coli with VP16 tag peptide (<a href='/en-us/products/unavailable/vp16-tag-peptide-ab13406'>ab13406</a>)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Applications

IHC-Fr, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody was raised against an immunogen that is predicted to react with HSV-1. We do not expect this product to react with HSV-2. Abcam welcomes customer feedback and would appreciate any comments regarding this product.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information VP16 tag

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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed35311648

2022

A light-gated transcriptional recorder for detecting cell-cell contacts.

Applications

Unspecified application

Species

Unspecified reactive species

Kelvin F Cho,Shawn M Gillespie,Nicholas A Kalogriopoulos,Michael A Quezada,Martin Jacko,Michelle Monje,Alice Y Ting

Journal of cellular and molecular medicine 26:880-892 PubMed34931449

2021

γ-Secretase modulators show selectivity for γ-secretase-mediated amyloid precursor protein intramembrane processing.

Applications

Unspecified application

Species

Unspecified reactive species

Tobias A Weber,Johan Lundkvist,Johanna Wanngren,Hlin Kvartsberg,ShaoBo Jin,Pia Larssen,Dan Wu,Daniel V Oliveira,Karolina Minta,Gunnar Brinkmalm,Henrik Zetterberg,Kaj Blennow,Gunnar Nordvall,Bengt Winblad,Erik Portelius,Helena Karlström

Molecular plant 13:717-731 PubMed32087370

2020

TOLs Function as Ubiquitin Receptors in the Early Steps of the ESCRT Pathway in Higher Plants.

Applications

Unspecified application

Species

Unspecified reactive species

Jeanette Moulinier-Anzola,Maximilian Schwihla,Lucinda De-Araújo,Christina Artner,Lisa Jörg,Nataliia Konstantinova,Christian Luschnig,Barbara Korbei

Bioscience, biotechnology, and biochemistry 84:970-979 PubMed31985350

2020

Flowering time control in rice by introducing Arabidopsis clock-associated PSEUDO-RESPONSE REGULATOR 5.

Applications

Unspecified application

Species

Unspecified reactive species

Norihito Nakamichi,Toru Kudo,Nobue Makita,Takatoshi Kiba,Toshinori Kinoshita,Hitoshi Sakakibara

Nucleic acids research 47:10662-10677 PubMed31586400

2019

A global functional analysis of missense mutations reveals two major hotspots in the PALB2 tumor suppressor.

Applications

Unspecified application

Species

Unspecified reactive species

Amélie Rodrigue,Guillaume Margaillan,Thiago Torres Gomes,Yan Coulombe,Gemma Montalban,Simone da Costa E Silva Carvalho,Larissa Milano,Mandy Ducy,Giuliana De-Gregoriis,Graham Dellaire,Wilson Araújo da Silva,Alvaro N Monteiro,Marcelo A Carvalho,Jacques Simard,Jean-Yves Masson

Plant direct 3:e00172 PubMed31549020

2019

Structure-function study of a novel inhibitor of the casein kinase 1 family in .

Applications

Unspecified application

Species

Unspecified reactive species

Ami N Saito,Hiromi Matsuo,Keiko Kuwata,Azusa Ono,Toshinori Kinoshita,Junichiro Yamaguchi,Norihito Nakamichi

Nature biotechnology 35:864-871 PubMed28650461

2017

A light- and calcium-gated transcription factor for imaging and manipulating activated neurons.

Applications

Species

Unspecified reactive species

Wenjing Wang,Craig P Wildes,Tanyaporn Pattarabanjird,Mateo I Sanchez,Gordon F Glober,Gillian A Matthews,Kay M Tye,Alice Y Ting

The Journal of cell biology 216:2329-2338 PubMed28619714

2017

The G protein-coupled receptor GPR31 promotes membrane association of KRAS.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole Fehrenbacher,Israel Tojal da Silva,Craig Ramirez,Yong Zhou,Kwang-Jin Cho,Shafi Kuchay,Jie Shi,Susan Thomas,Michele Pagano,John F Hancock,Dafna Bar-Sagi,Mark R Philips

Proceedings of the National Academy of Sciences of the United States of America 114:6310-6315 PubMed28559344

2017

Viral and cellular mRNA-specific activators harness PABP and eIF4G to promote translation initiation downstream of cap binding.

Applications

Unspecified application

Species

Unspecified reactive species

Richard W P Smith,Ross C Anderson,Osmany Larralde,Joel W S Smith,Barbara Gorgoni,William A Richardson,Poonam Malik,Sheila V Graham,Nicola K Gray

Bio-protocol 7:e2258 PubMed34541245

2017

Mating Based Split-ubiquitin Assay for Detection of Protein Interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Wijitra Horaruang,Ben Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VP16 tag antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VP16 tag antibody (AB4808)

Western blot - Anti-VP16 tag antibody (AB4808)

Key facts

Host species

Clonality

Isotype

Carrier free

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (27)

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise