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AB245743

Anti-VPAC1 antibody [SP234] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal VPAC1 antibody. Carrier free. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 1 publication.

View Alternative Names

Vasoactive intestinal polypeptide receptor 1, VIP-R-1, Pituitary adenylate cyclase-activating polypeptide type II receptor, VPAC1, PACAP type II receptor, PACAP-R-2, PACAP-R2, VIPR1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC1 antibody [SP234] - BSA and Azide free (AB245743)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC1 antibody [SP234] - BSA and Azide free (AB245743)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling VPAC1 with ab183312 at 1/100 dilution (1.01 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183312)

Flow Cytometry (Intracellular) - Anti-VPAC1 antibody [SP234] - BSA and Azide free (AB245743)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-VPAC1 antibody [SP234] - BSA and Azide free (AB245743)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for VIP Receptor using ab183312 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183312)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC1 antibody [SP234] - BSA and Azide free (AB245743)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC1 antibody [SP234] - BSA and Azide free (AB245743)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for VPAC1 using ab183312 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183312)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC1 antibody [SP234] - BSA and Azide free (AB245743)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC1 antibody [SP234] - BSA and Azide free (AB245743)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling VPAC1 with ab183312 at 1/100 dilution (1.01 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183312)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP234

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Primary antibody incubation for 10 minutes at room temperature.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p>Primary antibody incubation for 30 minutes at 4 degrees.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Primary antibody incubation for 10 minutes at room temperature.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This is a receptor for VIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The affinity is VIP = PACAP-27 > PACAP-38.
See full target information Vasoactive intestinal polypeptide receptor 1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Reproduction (Cambridge, England) 157:475-484 PubMed30817320

2019

VIP activates primordial follicles of rat through ERK-mTOR pathway in tissue culture.

Applications

Unspecified application

Species

Unspecified reactive species

Song Li,Qi Fan,Yanqiu Xie,Haiyan Lin,Qi Qiu,Yihua Liang,Qingxue Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB138260

Anti-VPAC1 antibody

primary-antibodies

vpac1-antibody-ab138260

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