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AB113683

Anti-VPH1 antibody [10D7A7B2]

3

(1 Review)

|

(12 Publications)

Mouse Monoclonal VPH1 antibody. Suitable for WB, ICC/IF and reacts with Saccharomyces cerevisiae samples. Cited in 12 publications.

View Alternative Names

YOR270C, VPH1, V-ATPase a 1 subunit, V-ATPase 95 kDa subunit, Vacuolar pH protein 1, Vacuolar proton pump a subunit, Vacuolar proton translocating ATPase subunit a 1

1 Images
Western blot - Anti-VPH1 antibody [10D7A7B2] (AB113683)
  • WB

Unknown

Western blot - Anti-VPH1 antibody [10D7A7B2] (AB113683)

All lanes:

Western blot - Anti-VPH1 antibody [10D7A7B2] (ab113683)

Lane 1:

Yeast whole cell lysate at 15 µg

Lane 2:

yeast membrane preparation at 15 µg

Predicted band size: 96 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

10D7A7B2

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Saccharomyces cerevisiae

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.25-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p>Heat induced antigen retrieval recommended.</p>" } } }
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Product details

This IgG2a kappa isotype control monoclonal antibody was raised in mouse against a yeast-specific protein, V-ATPase subunit (the product of VPH1 gene). It is a suitable isotype control for mammalian species.

Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody's host species, isotype, and possible conjugation.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed : 11278748, PubMed : 1491220, PubMed : 8798414). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments (PubMed : 11278748, PubMed : 1491220). Is present only in vacuolar V-ATPase complexes; enzymes containing this subunit have a 4-fold higher ratio of proton transport to ATP hydrolysis than complexes containing the Golgi/endosomal isoform and undergo reversible dissociation of V1 and V0 in response to glucose depletion (PubMed : 11278748, PubMed : 8798414).
See full target information VPH1
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107808 PubMed39307308

2024

Sphingolipids containing very long-chain fatty acids regulate Ypt7 function during the tethering stage of vacuole fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Chi Zhang,Jorge D Calderin,Logan R Hurst,Zeynep D Gokbayrak,Michael R Hrabak,Adam Balutowski,David A Rivera-Kohr,Thomas D D Kazmirchuk,Christopher L Brett,Rutilio A Fratti

Molecular biology of the cell 35:br20 PubMed39259764

2024

Big1 is a newly identified autophagy regulator that is critical for a fully functional V-ATPase.

Applications

Unspecified application

Species

Unspecified reactive species

Yuchen Lei,Ying Yang,Zhihai Zhang,Ruoxi Zhang,Xinxin Song,Sami N Malek,Daolin Tang,Daniel J Klionsky

The EMBO journal 43:1653-1685 PubMed38491296

2024

MemPrep, a new technology for isolating organellar membranes provides fingerprints of lipid bilayer stress.

Applications

Unspecified application

Species

Unspecified reactive species

John Reinhard,Leonhard Starke,Christian Klose,Per Haberkant,Henrik Hammarén,Frank Stein,Ofir Klein,Charlotte Berhorst,Heike Stumpf,James P Sáenz,Jochen Hub,Maya Schuldiner,Robert Ernst

The Journal of biological chemistry 299:102973 PubMed36738789

2023

The pH-sensing Rim101 pathway regulates cell size in budding yeast.

Applications

Unspecified application

Species

Unspecified reactive species

Masaru Shimasawa,Jun-Ichi Sakamaki,Tatsuya Maeda,Noboru Mizushima

STAR protocols 4:101935 PubMed36520633

2022

Protocol to purify and detect ubiquitinated phospholipids in budding yeast and human cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Ichi Sakamaki,Noboru Mizushima

PLoS genetics 16:e1009046 PubMed33064727

2020

The Ccr4-Not complex regulates TORC1 signaling and mitochondrial metabolism by promoting vacuole V-ATPase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Hongfeng Chen,P Winston Miller,Daniel L Johnson,R Nicholas Laribee

eLife 9: PubMed31909711

2020

The genetic basis of aneuploidy tolerance in wild yeast.

Applications

Unspecified application

Species

Unspecified reactive species

James Hose,Leah E Escalante,Katie J Clowers,H Auguste Dutcher,DeElegant Robinson,Venera Bouriakov,Joshua J Coon,Evgenia Shishkova,Audrey P Gasch

The Journal of biological chemistry 295:1439-1451 PubMed31844018

2019

Development of a yeast model to study the contribution of vacuolar polyphosphate metabolism to lysine polyphosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Azevedo,Yann Desfougères,Yannasittha Jiramongkol,Hamish Partington,Sasanan Trakansuebkul,Jyoti Singh,Nicole Steck,Henning J Jessen,Adolfo Saiardi

Developmental cell 47:80-97.e6 PubMed30269949

2018

Rab-Effector-Kinase Interplay Modulates Intralumenal Fragment Formation during Vacuole Fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Mahmoud Abdul Karim,Erin Kate McNally,Dieter Ronny Samyn,Sevan Mattie,Christopher Leonard Brett

Nature communications 8:15832 PubMed28607491

2017

An ubiquitin-dependent balance between mitofusin turnover and fatty acids desaturation regulates mitochondrial fusion.

Applications

WB

Species

Saccharomyces cerevisiae S288C

Laetitia Cavellini,Julie Meurisse,Justin Findinier,Zoi Erpapazoglou,Naïma Belgareh-Touzé,Allan M Weissman,Mickael M Cohen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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