Rabbit Recombinant Monoclonal VPS11 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.
Images
![Western blot - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (AB251005), expandable thumbnail](https://content.abcam.com/products/images/vps11-antibody-epr103442-bsa-and-azide-free-ab251005--western-blot-img104441.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (AB251005)")
![Western blot - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (AB251005), expandable thumbnail](https://content.abcam.com/products/images/vps11-antibody-epr103442-bsa-and-azide-free-ab251005--western-blot-img94071.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (AB251005)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (AB251005), expandable thumbnail](https://content.abcam.com/products/images/vps11-antibody-epr103442-bsa-and-azide-free-ab251005--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123053.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (AB251005)")
![Flow Cytometry (Intracellular) - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (AB251005), expandable thumbnail](https://content.abcam.com/products/images/vps11-antibody-epr103442-bsa-and-azide-free-ab251005--flow-cytometry-intracellular-img55157.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (AB251005)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed:11382755, PubMed:23351085, PubMed:24554770, PubMed:25266290, PubMed:25783203). Required for fusion of endosomes and autophagosomes with lysosomes (PubMed:25783203). Involved in cargo transport from early to late endosomes and required for the transition from early to late endosomes (PubMed:21148287). Involved in the retrograde Shiga toxin transport (PubMed:23593995).
Alternative names
RNF108, PP3476, VPS11, Vacuolar protein sorting-associated protein 11 homolog, hVPS11, RING finger protein 108
Recommended products
Rabbit Recombinant Monoclonal VPS11 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR10344(2)
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251005 is the carrier-free version of Anti-VPS11 antibody [EPR10344(2)] ab189920.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (ab251005), expandable thumbnail](https://content.abcam.com/products/images/vps11-antibody-epr103442-bsa-and-azide-free-ab251005--western-blot-img104441.jpg "Western blot - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (ab251005)")
Western blot - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (ab251005)
This data was developed using Anti-VPS11 antibody [EPR10344(2)] ab189920, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-VPS11 antibody [EPR10344(2)] (Anti-VPS11 antibody [EPR10344(2)] ab189920) at 1/10000 dilution
Lane 1: Human fetal brain tissue lysate at 20 µg
Lane 2: K562 cell lysate at 20 µg
Lane 3: 293 cell lysate at 20 µg
Lane 4: HeLa cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 108 kDa
Observed band size: 108 kDa
![Western blot - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (ab251005), expandable thumbnail](https://content.abcam.com/products/images/vps11-antibody-epr103442-bsa-and-azide-free-ab251005--western-blot-img94071.jpg "Western blot - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (ab251005)")
Western blot - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (ab251005)
This data was developed using Anti-VPS11 antibody [EPR10344(2)] ab189920, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-VPS11 antibody [EPR10344(2)] (Anti-VPS11 antibody [EPR10344(2)] ab189920) at 1/1000 dilution
All lanes: RAW 264.7 cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 108 kDa
Observed band size: 108 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (ab251005), expandable thumbnail](https://content.abcam.com/products/images/vps11-antibody-epr103442-bsa-and-azide-free-ab251005--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123053.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (ab251005)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (ab251005)
This data was developed using Anti-VPS11 antibody [EPR10344(2)] ab189920, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling VPS11 with Anti-VPS11 antibody [EPR10344(2)] ab189920 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (ab251005), expandable thumbnail](https://content.abcam.com/products/images/vps11-antibody-epr103442-bsa-and-azide-free-ab251005--flow-cytometry-intracellular-img55157.jpg "Flow Cytometry (Intracellular) - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (ab251005)")
Flow Cytometry (Intracellular) - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (ab251005)
This data was developed using Anti-VPS11 antibody [EPR10344(2)] ab189920, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling VPS11 with Anti-VPS11 antibody [EPR10344(2)] ab189920 at 1/90 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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