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AB251005

Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal VPS11 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

RNF108, PP3476, VPS11, Vacuolar protein sorting-associated protein 11 homolog, hVPS11, RING finger protein 108

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (AB251005)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (AB251005)

This data was developed using ab189920, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling VPS11 with ab189920 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (AB251005)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (AB251005)

This data was developed using ab189920, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling VPS11 with ab189920 at 1/90 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Western blot - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (AB251005)
  • WB

Supplier Data

Western blot - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (AB251005)

This data was developed using ab189920, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-VPS11 antibody [EPR10344(2)] (<a href='/en-us/products/primary-antibodies/vps11-antibody-epr103442-ab189920'>ab189920</a>) at 1/10000 dilution

Lane 1:

Human fetal brain tissue lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 108 kDa

Observed band size: 108 kDa

false

Western blot - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (AB251005)
  • WB

Supplier Data

Western blot - Anti-VPS11 antibody [EPR10344(2)] - BSA and Azide free (AB251005)

This data was developed using ab189920, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-VPS11 antibody [EPR10344(2)] (<a href='/en-us/products/primary-antibodies/vps11-antibody-epr103442-ab189920'>ab189920</a>) at 1/1000 dilution

All lanes:

RAW 264.7 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 108 kDa

Observed band size: 108 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10344(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab251005 is the carrier-free version of ab189920.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed : 11382755, PubMed : 23351085, PubMed : 24554770, PubMed : 25266290, PubMed : 25783203). Required for fusion of endosomes and autophagosomes with lysosomes (PubMed : 25783203). Involved in cargo transport from early to late endosomes and required for the transition from early to late endosomes (PubMed : 21148287). Involved in the retrograde Shiga toxin transport (PubMed : 23593995).
See full target information VPS11
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Genes 14: PubMed37239471

2023

Differential Regulation of POC5 by ERα in Human Normal and Scoliotic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Amani Hassan,Edward T Bagu,Shunmoogum A Patten,Sirinart Molidperee,Stefan Parent,Soraya Barchi,Isabelle Villemure,André Tremblay,Florina Moldovan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com