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AB170869

Anti-VPS11 antibody [EPR10345]

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(2 Publications)

Rabbit Recombinant Monoclonal VPS11 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

RNF108, PP3476, VPS11, Vacuolar protein sorting-associated protein 11 homolog, hVPS11, RING finger protein 108

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-VPS11 antibody [EPR10345] (AB170869)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VPS11 antibody [EPR10345] (AB170869)

Immunofluorescent staining of HeLa cells labeling VPS11 using ab170869 at a 1/100 dilution (red) and DAPI nuclear staining (blue).

Immunoprecipitation - Anti-VPS11 antibody [EPR10345] (AB170869)
  • IP

Supplier Data

Immunoprecipitation - Anti-VPS11 antibody [EPR10345] (AB170869)

All lanes:

Immunoprecipitation - Anti-VPS11 antibody [EPR10345] (ab170869) at 1/10 dilution

All lanes:

HEK-293T cell lysate

Predicted band size: 108 kDa

false

Western blot - Anti-VPS11 antibody [EPR10345] (AB170869)
  • WB

Supplier Data

Western blot - Anti-VPS11 antibody [EPR10345] (AB170869)

All lanes:

Western blot - Anti-VPS11 antibody [EPR10345] (ab170869) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 108 kDa

false

  • Carrier free

    Anti-VPS11 antibody [EPR10345] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10345

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed : 11382755, PubMed : 23351085, PubMed : 24554770, PubMed : 25266290, PubMed : 25783203). Required for fusion of endosomes and autophagosomes with lysosomes (PubMed : 25783203). Involved in cargo transport from early to late endosomes and required for the transition from early to late endosomes (PubMed : 21148287). Involved in the retrograde Shiga toxin transport (PubMed : 23593995).
See full target information VPS11
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 35:ar40 PubMed38198575

2024

Loss of the HOPS complex disrupts early-to-late endosome transition, impairs endosomal recycling and induces accumulation of amphisomes.

Applications

Unspecified application

Species

Unspecified reactive species

Jan van der Beek,Cecilia de Heus,Paolo Sanza,Nalan Liv,Judith Klumperman

Nature communications 9:4279 PubMed30323255

2018

Mrp1 is involved in lipid presentation and iNKT cell activation by Streptococcus pneumoniae.

Applications

Unspecified application

Species

Unspecified reactive species

Shilpi Chandra,James Gray,William B Kiosses,Archana Khurana,Kaori Hitomi,Catherine M Crosby,Ashu Chawla,Zheng Fu,Meng Zhao,Natacha Veerapen,Stewart K Richardson,Steven A Porcelli,Gurdyal Besra,Amy R Howell,Sonia Sharma,Bjoern Peters,Mitchell Kronenberg
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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