JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB249551

Anti-VPS11 antibody [EPR10345] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal VPS11 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.

View Alternative Names

RNF108, PP3476, VPS11, Vacuolar protein sorting-associated protein 11 homolog, hVPS11, RING finger protein 108

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-VPS11 antibody [EPR10345] - BSA and Azide free (AB249551)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VPS11 antibody [EPR10345] - BSA and Azide free (AB249551)

This data was developed using ab170869, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of HeLa cells labeling VPS11 using ab170869 at a 1/100 dilution (red) and DAPI nuclear staining (blue).

Immunoprecipitation - Anti-VPS11 antibody [EPR10345] - BSA and Azide free (AB249551)
  • IP

Supplier Data

Immunoprecipitation - Anti-VPS11 antibody [EPR10345] - BSA and Azide free (AB249551)

This data was developed using ab170869, the same antibody clone in a different buffer formulation.

All lanes:

Immunoprecipitation - Anti-VPS11 antibody [EPR10345] (<a href='/en-us/products/primary-antibodies/vps11-antibody-epr10345-ab170869'>ab170869</a>) at 1/10 dilution

All lanes:

HEK-293T cell lysate

Predicted band size: 108 kDa

false

Western blot - Anti-VPS11 antibody [EPR10345] - BSA and Azide free (AB249551)
  • WB

Supplier Data

Western blot - Anti-VPS11 antibody [EPR10345] - BSA and Azide free (AB249551)

This data was developed using ab170869, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-VPS11 antibody [EPR10345] (<a href='/en-us/products/primary-antibodies/vps11-antibody-epr10345-ab170869'>ab170869</a>) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 108 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10345

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB125084

Anti-C14orf133 antibody

Immunoprecipitation - Anti-C14orf133 antibody (AB125084)

  • 0
  • 0
Primary Antibodies

AB202285

Anti-VPS13D antibody

Immunoprecipitation - Anti-VPS13D antibody (AB202285)

  • 0
  • 0
Primary Antibodies

AB36195

Anti-SYBL1/VAMP-7 antibody [158.2]

Flow Cytometry - Anti-SYBL1/VAMP-7 antibody [158.2] (AB36195)

  • 5
  • 1
Primary Antibodies

AB192890

Anti-LC3B antibody [EPR18709] - Autophagosome Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-LC3B antibody [EPR18709] - Autophagosome Marker (AB192890)

  • 5
  • 19
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

ab249551 is the carrier-free version of ab170869.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed : 11382755, PubMed : 23351085, PubMed : 24554770, PubMed : 25266290, PubMed : 25783203). Required for fusion of endosomes and autophagosomes with lysosomes (PubMed : 25783203). Involved in cargo transport from early to late endosomes and required for the transition from early to late endosomes (PubMed : 21148287). Involved in the retrograde Shiga toxin transport (PubMed : 23593995).
See full target information VPS11
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com