AB202285

Anti-VPS13D antibody

Name: Anti-VPS13D antibody (ab202285) | Abcam
Brand: Abcam Limited
SKU: AB202285
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal VPS13D antibody. Suitable for IP and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human VPS13D aa 2150-2200.

View Alternative Names

KIAA0453, VPS13D, Intermembrane lipid transfer protein VPS13D, Vacuolar protein sorting-associated protein 13D

1 Images

Supplier Data

Immunoprecipitation - Anti-VPS13D antibody (AB202285)

Lane 1 : Detection of Human VPS13D by Immunoprecipitation from Jurkat whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using ab202285 at 6 μg per reaction for IP.

Lane 2 : Immunoprecipitation from Jurkat whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using an antibody which recognized an epitope of VPS13D.
Lane 3 : Immunoprecipitation from Jurkat whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab202285 at 1 μg/ml.

Detection : Chemiluminescence with an exposure time of 3
minutes.

All lanes:

Immunoprecipitation - Anti-VPS13D antibody (ab202285)

Predicted band size: 492 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human VPS13D aa 2150-2200. The exact immunogen used to generate this antibody is proprietary information.

Q5THJ4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab202285 was affinity purified using an epitope specific to VPS13D immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates the transfer of lipids between membranes at organelle contact sites (By similarity). Functions in promoting mitochondrial clearance by mitochondrial autophagy (mitophagy), also possibly by positively regulating mitochondrial fission (PubMed : 29307555, PubMed : 29604224). Mitophagy plays an important role in regulating cell health and mitochondrial size and homeostasis.

See full target information VPS13D

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 24:e55895 PubMed37317656

2023

C9orf72 protein quality control by UBR5-mediated heterotypic ubiquitin chains.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Jülg,Dieter Edbauer,Christian Behrends

eLife 11: PubMed36448541

2022

An ER phospholipid hydrolase drives ER-associated mitochondrial constriction for fission and fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Tricia T Nguyen,Gia K Voeltz

Cell reports 39:110776 PubMed35508140

2022

Cholesterol determines the cytosolic entry and seeded aggregation of tau.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin J Tuck,Lauren V C Miller,Taxiarchis Katsinelos,Annabel E Smith,Emma L Wilson,Sophie Keeling,Shi Cheng,Marina J Vaysburd,Claire Knox,Lucy Tredgett,Emmanouil Metzakopian,Leo C James,William A McEwan

The Journal of cell biology 220: PubMed33891013

2021

VPS13D bridges the ER to mitochondria and peroxisomes via Miro.

Applications

Unspecified application

Species

Unspecified reactive species

Andrés Guillén-Samander,Marianna Leonzino,Michael G Hanna,Ni Tang,Hongying Shen,Pietro De Camilli

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VPS13D antibody

Immunoprecipitation - Anti-VPS13D antibody (AB202285)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

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