Anti-VPS15 antibody - C-terminal

• IP

Supplier Data

Immunoprecipitation - Anti-VPS15 antibody - C-terminal (AB229665)

VPS15 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 μg of ab229665. Western blot was performed from the immunoprecipitate using ab229665. Anti-rabbit IgG was used as the secondary antibody.

Lane 1 : HeLa whole cell extract (input).
Lane 2 : Control IgG instead of ab229665 in HeLa whole cell extract.
Lane 3 : ab229665 IP in HeLa whole cell extract.

All lanes:

Immunoprecipitation - Anti-VPS15 antibody - C-terminal (ab229665)

Predicted band size: 153 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS15 antibody - C-terminal (AB229665)

Paraffin-embedded mouse duodenum tissue stained for VPS15 using ab229665 at 1/500 dilution in immunohistochemical analysis.

• WB

Supplier Data

Western blot - Anti-VPS15 antibody - C-terminal (AB229665)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-VPS15 antibody - C-terminal (ab229665) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract at 30 µg

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell extract at 30 µg

Lane 3:

NCI-H929 (human bone marrow lymphoblast cell line) whole cell extract at 30 µg

Predicted band size: 153 kDa

false

• WB

Supplier Data

Western blot - Anti-VPS15 antibody - C-terminal (AB229665)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-VPS15 antibody - C-terminal (ab229665) at 1/1000 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

PIK3R4 shRNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Predicted band size: 153 kDa

false

• WB

Supplier Data

Western blot - Anti-VPS15 antibody - C-terminal (AB229665)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-VPS15 antibody - C-terminal (ab229665) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Predicted band size: 153 kDa

false

• WB

Supplier Data

Western blot - Anti-VPS15 antibody - C-terminal (AB229665)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-VPS15 antibody - C-terminal (ab229665) at 1/1000 dilution

All lanes:

Mouse liver tissue extract at 50 µg

Predicted band size: 153 kDa

false

• WB

Supplier Data

Western blot - Anti-VPS15 antibody - C-terminal (AB229665)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-VPS15 antibody - C-terminal (ab229665) at 1/1000 dilution

Lane 1:

Neuro-2a (Mouse neuroblastoma cell line) whole cell extract at 30 µg

Lane 2:

C8D30 whole cell extract at 30 µg

Lane 3:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell extract at 30 µg

Lane 4:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg

Lane 5:

C2C12 (Mouse myoblast cell line) whole cell extract at 30 µg

Predicted band size: 153 kDa

false