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Rabbit Recombinant Monoclonal VPS15 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human, Rat samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Not recommended
Mouse
Tested
Not recommended
Rat
Tested
Not recommended

Tested
Tested

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Target data

Function

Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123).

Alternative names

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Rabbit Recombinant Monoclonal VPS15 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human, Rat samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR5302(2)

Purification technique

Affinity purification Protein A

Dissociation constant

1.22 x 10-10 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab248210 is the carrier-free version of ab128903.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-VPS15 antibody [EPR5302(2)] - BSA and Azide free (ab248210), expandable thumbnail

    Western blot - Anti-VPS15 antibody [EPR5302(2)] - BSA and Azide free (ab248210)

    This antibody detects high background.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128903).

    All lanes: Western blot - Anti-VPS15 antibody [EPR5302(2)] (AB128903) at 1/1000 dilution

    All lanes: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 153 kDa

    Observed band size: 150 kDa

  • Western blot - Anti-VPS15 antibody [EPR5302(2)] - BSA and Azide free (ab248210), expandable thumbnail

    Western blot - Anti-VPS15 antibody [EPR5302(2)] - BSA and Azide free (ab248210)

    This antibody detects high background.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128903).

    All lanes: Western blot - Anti-VPS15 antibody [EPR5302(2)] (AB128903) at 1/500 dilution

    Lane 1: Mouse E14.5 brain lysates at 20 µg

    Lane 2: Rat E14 brain lysates at 20 µg

    Lane 3: Rat P0 brain lysates at 20 µg

    Lane 4: C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

    Lane 5: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 153 kDa

    Observed band size: 150 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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