AB124817

Anti-VPS15 antibody [EPR5303(2)]

Name: Anti-VPS15 antibody [EPR5303(2)] (ab124817) | Abcam
Brand: Abcam Limited
SKU: AB124817
Price: 355 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(3 Publications)

Rabbit Recombinant Monoclonal VPS15 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

VPS15, PIK3R4, Phosphoinositide 3-kinase regulatory subunit 4, PI3-kinase regulatory subunit 4, PI3-kinase p150 subunit, Phosphoinositide 3-kinase adaptor protein

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS15 antibody [EPR5303(2)] (AB124817)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS15 antibody [EPR5303(2)] (AB124817)

ab124817 at 1/100 dilution staining VPS15 in Formalin fixed Paraffin-embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-VPS15 antibody [EPR5303(2)] (AB124817)

All lanes:

Western blot - Anti-VPS15 antibody [EPR5303(2)] (ab124817) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

Molt-4 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Lane 5:

C6 cell lysate at 10 µg

Lane 6:

Raw 264.7 cell lysate at 10 µg

Secondary

All lanes:

Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 153 kDa

false

Carrier free

Anti-VPS15 antibody [EPR5303(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5303(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways : PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed : 20643123).

See full target information PIK3R4

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0317369 PubMed40063597

2025

Identification and validation of the common pathogenesis and hub biomarkers in Papillary thyroid carcinoma complicated by rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Yingming Liu,Xiangjun Kong,Qianshu Sun,Tianxing Cui,Shengnan Xu,Chao Ding

Autophagy 20:1798-1814 PubMed38705725

2024

The mammalian actin elongation factor ENAH/MENA contributes to autophagosome formation via its actin regulatory function.

Applications

Unspecified application

Species

Unspecified reactive species

Yueheng Li,Yafei Zhang,Menghui Wang,Junhui Su,Xinjue Dong,Yuqi Yang,Hongshan Wang,QingQuan Li

Nature neuroscience 21:207-217 PubMed29311744

2018

Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Gstrein,Andrew Edwards,Anna Přistoupilová,Ines Leca,Martin Breuss,Sandra Pilat-Carotta,Andi H Hansen,Ratna Tripathy,Anna K Traunbauer,Tobias Hochstoeger,Gavril Rosoklija,Marco Repic,Lukas Landler,Viktor Stránecký,Gerhard Dürnberger,Thomas M Keane,Johannes Zuber,David J Adams,Jonathan Flint,Tomas Honzik,Marta Gut,Sergi Beltran,Karl Mechtler,Elliott Sherr,Stanislav Kmoch,Ivo Gut,David A Keays

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VPS15 antibody [EPR5303(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS15 antibody [EPR5303(2)] (AB124817)

Western blot - Anti-VPS15 antibody [EPR5303(2)] (AB124817)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (3)

Product promise