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AB206326

Anti-VPS16 antibody

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(2 Publications)

Goat Polyclonal VPS16 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human VPS16 aa 350-400.

View Alternative Names

Vacuolar protein sorting-associated protein 16 homolog, hVPS16, VPS16

2 Images
Western blot - Anti-VPS16 antibody (AB206326)
  • WB

Supplier Data

Western blot - Anti-VPS16 antibody (AB206326)

All lanes:

Western blot - Anti-VPS16 antibody (ab206326) at 0.1 µg/mL

All lanes:

mouse spleen lysate (in RIPA buffer) at 35 µg

Predicted band size: 95 kDa

Observed band size: 100 kDa

true

Western blot - Anti-VPS16 antibody (AB206326)
  • WB

Supplier Data

Western blot - Anti-VPS16 antibody (AB206326)

All lanes:

Western blot - Anti-VPS16 antibody (ab206326) at 0.1 µg/mL

Lane 1:

HeLa lysate (in RIPA buffer) at 35 µg

Lane 2:

HepG2 lysate (in RIPA buffer) at 35 µg

Lane 3:

Jurkat lysate (in RIPA buffer) at 35 µg

Predicted band size: 95 kDa

Observed band size: 100 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human VPS16 aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

Q9H269

Specificity

ab206326 is expected to recognize reported isoform 1 (NP_072097.2) only.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab206326 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed : 11382755, PubMed : 23351085, PubMed : 24554770, PubMed : 25266290, PubMed : 25783203). Required for recruitment of VPS33A to the HOPS complex (PubMed : 23901104). Required for fusion of endosomes and autophagosomes with lysosomes; the function is dependent on its association with VPS33A but not VPS33B (PubMed : 25783203). The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG (PubMed : 24554770).
See full target information VPS16
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 12:e12319 PubMed37021404

2023

Exosomal lipid PI4P regulates small extracellular vesicle secretion by modulating intraluminal vesicle formation.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Jin,Tian Xia,Shuchen Luo,Ying Zhang,Yu Xia,Hang Yin

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 35:1322-1332 PubMed32119750

2020

Adult Osteosclerotic Metaphyseal Dysplasia With Progressive Osteonecrosis of the Jaws and Abnormal Bone Resorption Pattern Due to a LRRK1 Splice Site Mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Antonia Howaldt,Anna Floriane Hennig,Tim Rolvien,Uta Rössler,Nina Stelzer,Alexej Knaus,Sebastian Böttger,Jozef Zustin,Sven Geißler,Ralf Oheim,Michael Amling,Hans-Peter Howaldt,Uwe Kornak
View all publications
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Product promise

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