Rabbit Recombinant Monoclonal VPS18 antibody. N-terminal. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.
Images
![Western blot - Anti-VPS18 antibody [EPR13378] - N-terminal (AB178416), expandable thumbnail](https://content.abcam.com/products/images/vps18-antibody-epr13378-n-terminal-ab178416--western-blot-img126801.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VPS18 antibody [EPR13378] - N-terminal (AB178416)")
![Immunocytochemistry/ Immunofluorescence - Anti-VPS18 antibody [EPR13378] - N-terminal (AB178416), expandable thumbnail](https://content.abcam.com/products/images/vps18-antibody-epr13378-n-terminal-ab178416--immunocytochemistry-immunofluorescence-img1127.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-VPS18 antibody [EPR13378] - N-terminal (AB178416)")
![Flow Cytometry (Intracellular) - Anti-VPS18 antibody [EPR13378] - N-terminal (AB178416), expandable thumbnail](https://content.abcam.com/products/images/vps18-antibody-epr13378-n-terminal-ab178416--flow-cytometry-intracellular-img26609.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-VPS18 antibody [EPR13378] - N-terminal (AB178416)")
Publications
Filter by
- Nature communications 14:63602023C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShen Zhang,Mindan Tong,Denghao Zheng,Huiying Huang,Linsen Li,Christian Ungermann,Yi Pan,Hanyan Luo,Ming Lei,Zaiming Tang,Wan Fu,She Chen,Xiaoxia Liu,Qing ZhongPubMed 37821429
- The EMBO journal 39:e1023012020SKIP-HOPS recruits TBC1D15 for a Rab7-to-Arl8b identity switch to control late endosome transport.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarlieke Lm Jongsma et. al.PubMed 32080880
- PLoS pathogens 13:e10067002017Salmonella exploits the host endolysosomal tethering factor HOPS complex to promote its intravacuolar replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAastha Sindhwani et. al.PubMed 29084291
- The Journal of cell biology 216:1051-10702017The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRituraj Marwaha et. al.PubMed 28325809
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested | Tested |
| Mouse | Not recommended | Predicted | Not recommended | Predicted | Predicted |
| Rat | Not recommended | Predicted | Not recommended | Predicted | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed:11382755, PubMed:23351085, PubMed:24554770, PubMed:25783203). Required for fusion of endosomes and autophagosomes with lysosomes (PubMed:25783203). Involved in dendrite development of Pukinje cells (By similarity).
Alternative names
KIAA1475, VPS18, Vacuolar protein sorting-associated protein 18 homolog, hVPS18
Recommended products
Rabbit Recombinant Monoclonal VPS18 antibody. N-terminal. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR13378
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416), expandable thumbnail](https://content.abcam.com/products/images/vps18-antibody-epr13378-n-terminal-ab178416--western-blot-img126801.jpg "Western blot - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)")
Western blot - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)
All lanes: Western blot - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg
Lane 3: K562 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 110 kDa
Observed band size: 100 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416), expandable thumbnail](https://content.abcam.com/products/images/vps18-antibody-epr13378-n-terminal-ab178416--immunocytochemistry-immunofluorescence-img1127.jpg "Immunocytochemistry/ Immunofluorescence - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)")
Immunocytochemistry/ Immunofluorescence - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)
Immunofluorescent analysis of 293T cells labeling VPS18 with ab178416 at 1/100 (green) and DAPI staining (blue).
![Flow Cytometry (Intracellular) - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416), expandable thumbnail](https://content.abcam.com/products/images/vps18-antibody-epr13378-n-terminal-ab178416--flow-cytometry-intracellular-img26609.jpg "Flow Cytometry (Intracellular) - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)")
Flow Cytometry (Intracellular) - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)
Intracellular flow cytometrical analysis of permeabilized K562 cells labeling VPS18 with ab178416 at 1/10 (red) or a rabbit IgG (negative) (green).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com