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AB178416

Anti-VPS18 antibody [EPR13378] - N-terminal

4

(1 Review)

|

(7 Publications)

Rabbit Recombinant Monoclonal VPS18 antibody. N-terminal. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.

View Alternative Names

KIAA1475, VPS18, Vacuolar protein sorting-associated protein 18 homolog, hVPS18

3 Images
Flow Cytometry (Intracellular) - Anti-VPS18 antibody [EPR13378] - N-terminal (AB178416)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-VPS18 antibody [EPR13378] - N-terminal (AB178416)

Intracellular flow cytometrical analysis of permeabilized K562 cells labeling VPS18 with ab178416 at 1/10 (red) or a rabbit IgG (negative) (green).

Immunocytochemistry/ Immunofluorescence - Anti-VPS18 antibody [EPR13378] - N-terminal (AB178416)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VPS18 antibody [EPR13378] - N-terminal (AB178416)

Immunofluorescent analysis of 293T cells labeling VPS18 with ab178416 at 1/100 (green) and DAPI staining (blue).

Western blot - Anti-VPS18 antibody [EPR13378] - N-terminal (AB178416)
  • WB

Supplier Data

Western blot - Anti-VPS18 antibody [EPR13378] - N-terminal (AB178416)

All lanes:

Western blot - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 110 kDa

Observed band size: 100 kDa

false

  • Carrier free

    Anti-VPS18 antibody [EPR13378] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13378

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed : 11382755, PubMed : 23351085, PubMed : 24554770, PubMed : 25783203). Required for fusion of endosomes and autophagosomes with lysosomes (PubMed : 25783203). Involved in dendrite development of Pukinje cells (By similarity).
See full target information VPS18
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 224: PubMed39907656

2025

VPS41 recruits biosynthetic LAMP-positive vesicles through interaction with Arl8b.

Applications

Unspecified application

Species

Unspecified reactive species

Paolo Sanzà,Jan van der Beek,Derk Draper,Cecilia de Heus,Tineke Veenendaal,Corlinda Ten Brink,Ginny G Farías,Nalan Liv,Judith Klumperman

Science advances 11:eadr6166 PubMed39888996

2025

VPS18 contributes to phagosome membrane integrity in -infected macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Charul Jani,Neha Jain,Amanda K Marsh,Pooja Uchil,Triet Doan,Meggie Hudspith,Owen T Glover,Zach R Baskir,Julie Boucau,David E Root,Nicole N van der Wel,John G Doench,Amy K Barczak

Molecular biology of the cell 35:ar40 PubMed38198575

2024

Loss of the HOPS complex disrupts early-to-late endosome transition, impairs endosomal recycling and induces accumulation of amphisomes.

Applications

Unspecified application

Species

Unspecified reactive species

Jan van der Beek,Cecilia de Heus,Paolo Sanza,Nalan Liv,Judith Klumperman

Nature communications 14:6360 PubMed37821429

2023

C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Shen Zhang,Mindan Tong,Denghao Zheng,Huiying Huang,Linsen Li,Christian Ungermann,Yi Pan,Hanyan Luo,Ming Lei,Zaiming Tang,Wan Fu,She Chen,Xiaoxia Liu,Qing Zhong

The EMBO journal 39:e102301 PubMed32080880

2020

SKIP-HOPS recruits TBC1D15 for a Rab7-to-Arl8b identity switch to control late endosome transport.

Applications

Unspecified application

Species

Unspecified reactive species

Marlieke Lm Jongsma,Jeroen Bakker,Birol Cabukusta,Nalan Liv,Daphne van Elsland,Job Fermie,Jimmy Ll Akkermans,Coenraad Kuijl,Sabina Y van der Zanden,Lennert Janssen,Denise Hoogzaad,Rik van der Kant,Ruud H Wijdeven,Judith Klumperman,Ilana Berlin,Jacques Neefjes

PLoS pathogens 13:e1006700 PubMed29084291

2017

Salmonella exploits the host endolysosomal tethering factor HOPS complex to promote its intravacuolar replication.

Applications

Unspecified application

Species

Unspecified reactive species

Aastha Sindhwani,Subhash B Arya,Harmeet Kaur,Divya Jagga,Amit Tuli,Mahak Sharma

The Journal of cell biology 216:1051-1070 PubMed28325809

2017

The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Rituraj Marwaha,Subhash B Arya,Divya Jagga,Harmeet Kaur,Amit Tuli,Mahak Sharma
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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