JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB178689

Anti-VPS18 antibody [EPR13379]

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal VPS18 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

KIAA1475, VPS18, Vacuolar protein sorting-associated protein 18 homolog, hVPS18

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-VPS18 antibody [EPR13379] (AB178689)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VPS18 antibody [EPR13379] (AB178689)

Immunofluorescence analysis of A549 cells labeling VPS18 with ab178689 at a 1/100 dilution. DAPI nuclear staining (blue).

Flow Cytometry (Intracellular) - Anti-VPS18 antibody [EPR13379] (AB178689)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-VPS18 antibody [EPR13379] (AB178689)

Intracellular flow cytometric analysis of permeabilized 293T cells using ab178689 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).

Western blot - Anti-VPS18 antibody [EPR13379] (AB178689)
  • WB

Supplier Data

Western blot - Anti-VPS18 antibody [EPR13379] (AB178689)

All lanes:

Western blot - Anti-VPS18 antibody [EPR13379] (ab178689) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Predicted band size: 110 kDa

false

  • Carrier free

    Anti-VPS18 antibody [EPR13379] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13379

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
style
left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed : 11382755, PubMed : 23351085, PubMed : 24554770, PubMed : 25783203). Required for fusion of endosomes and autophagosomes with lysosomes (PubMed : 25783203). Involved in dendrite development of Pukinje cells (By similarity).
See full target information VPS18
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 23:277 PubMed37978523

2023

Eleven metabolism‑related genes composed of Stard5 predict prognosis and contribute to EMT phenotype in HCC.

Applications

Unspecified application

Species

Unspecified reactive species

Dongping Li,Xiahui Lin,Jiale Li,Xinyi Liu,Feng Zhang,Wenqing Tang,Si Zhang,Ling Dong,Ruyi Xue

Life science alliance 6: PubMed36316035

2022

CRISPRi screening reveals regulators of tau pathology shared between exosomal and vesicle-free tau.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Carlos Polanco,Yevhen Akimov,Avinash Fernandes,Adam Briner,Gabriel Rhys Hand,Marloes van Roijen,Giuseppe Balistreri,Jürgen Götz
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB240574

Anti-VPS18 antibody

Immunoprecipitation - Anti-VPS18 antibody (AB240574)

  • 0
  • 0
Primary Antibodies

AB178416

Anti-VPS18 antibody [EPR13378] - N-terminal

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-VPS18 antibody [EPR13378] - N-terminal (AB178416)

  • 4
  • 1

Complementary Products

Primary Antibodies

AB241382

Anti-YKT6 antibody

Immunoprecipitation - Anti-YKT6 antibody (AB241382)

  • 0
  • 0
Primary Antibodies

AB133497

Anti-Flotillin 1 antibody [EPR6041]

RabMAb|Recombinant|KO Validated|20ul selling size

Flow Cytometry (Intracellular) - Anti-Flotillin 1 antibody [EPR6041] (AB133497)

  • 5
  • 6
Primary Antibodies

AB181078

Anti-VPS41 antibody [EPR13268]

RabMAb|Recombinant

Western blot - Anti-VPS41 antibody [EPR13268] (AB181078)

  • 5
  • 1
Primary Antibodies

AB177477

Anti-IMP3 antibody [EPR12021]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-IMP3 antibody [EPR12021] (AB177477)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com