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AB250063

Anti-VPS18 antibody [EPR13379] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal VPS18 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA1475, VPS18, Vacuolar protein sorting-associated protein 18 homolog, hVPS18

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-VPS18 antibody [EPR13379] - BSA and Azide free (AB250063)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VPS18 antibody [EPR13379] - BSA and Azide free (AB250063)

This data was developed using ab178689, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of A549 cells labeling VPS18 with ab178689 at a 1/100 dilution. DAPI nuclear staining (blue).

Flow Cytometry (Intracellular) - Anti-VPS18 antibody [EPR13379] - BSA and Azide free (AB250063)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-VPS18 antibody [EPR13379] - BSA and Azide free (AB250063)

This data was developed using ab178689, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized 293T cells using ab178689 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).

Western blot - Anti-VPS18 antibody [EPR13379] - BSA and Azide free (AB250063)
  • WB

Supplier Data

Western blot - Anti-VPS18 antibody [EPR13379] - BSA and Azide free (AB250063)

This data was developed using ab178689, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-VPS18 antibody [EPR13379] (<a href='/en-us/products/primary-antibodies/vps18-antibody-epr13379-ab178689'>ab178689</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Predicted band size: 110 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13379

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab250063 is the carrier-free version of ab178689.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed : 11382755, PubMed : 23351085, PubMed : 24554770, PubMed : 25783203). Required for fusion of endosomes and autophagosomes with lysosomes (PubMed : 25783203). Involved in dendrite development of Pukinje cells (By similarity).
See full target information VPS18
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 3: PubMed25206958

2014

Recovery of extracellular vesicles from human breast milk is influenced by sample collection and vesicle isolation procedures.

Applications

Unspecified application

Species

Unspecified reactive species

Marijke I Zonneveld,Alain R Brisson,Martijn J C van Herwijnen,Sisareuth Tan,Chris H A van de Lest,Frank A Redegeld,Johan Garssen,Marca H M Wauben,Esther N M Nolte-'t Hoen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com