Rabbit Recombinant Monoclonal VPS24 antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (AB249897), expandable thumbnail](https://content.abcam.com/products/images/vps24-antibody-epr12821-bsa-and-azide-free-ab249897--western-blot-img115394.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (AB249897)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (AB249897), expandable thumbnail](https://content.abcam.com/products/images/vps24-antibody-epr12821-bsa-and-azide-free-ab249897--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img40244.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (AB249897)")
![Immunoprecipitation - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (AB249897), expandable thumbnail](https://content.abcam.com/products/images/vps24-antibody-epr12821-bsa-and-azide-free-ab249897--immunoprecipitation-img73856.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (AB249897)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (AB249897), expandable thumbnail](https://content.abcam.com/products/images/vps24-antibody-epr12821-bsa-and-azide-free-ab249897--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116687.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (AB249897)")
![Flow Cytometry (Intracellular) - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (AB249897), expandable thumbnail](https://content.abcam.com/products/images/vps24-antibody-epr12821-bsa-and-azide-free-ab249897--flow-cytometry-intracellular-img27172.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (AB249897)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Tested |
| Mouse | Predicted | Predicted | Predicted | Not recommended | Predicted |
| Rat | Predicted | Predicted | Predicted | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Plays a role in endosomal sorting/trafficking of EGF receptor. Isoform 2 prevents stress-mediated cell death and accumulation of reactive oxygen species when expressed in yeast cells.
Alternative names
CGI149, NEDF, VPS24, CGI-149, CHMP3, Charged multivesicular body protein 3, Chromatin-modifying protein 3, Neuroendocrine differentiation factor, Vacuolar protein sorting-associated protein 24, hVps24
Recommended products
Rabbit Recombinant Monoclonal VPS24 antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12821
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249897 is the carrier-free version of Anti-VPS24 antibody [EPR12821] ab175930.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (ab249897), expandable thumbnail](https://content.abcam.com/products/images/vps24-antibody-epr12821-bsa-and-azide-free-ab249897--western-blot-img115394.jpg "Western blot - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (ab249897)")
Western blot - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (ab249897)
This data was developed using Anti-VPS24 antibody [EPR12821] ab175930, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-VPS24 antibody [EPR12821] (Anti-VPS24 antibody [EPR12821] ab175930) at 1/1000 dilution
Lane 1: 293T cell lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: U87-MG cell lysate at 10 µg
Lane 4: Fetal brain tissue lysate at 10 µg
Predicted band size: 25 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (ab249897), expandable thumbnail](https://content.abcam.com/products/images/vps24-antibody-epr12821-bsa-and-azide-free-ab249897--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img40244.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (ab249897)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (ab249897)
This data was developed using Anti-VPS24 antibody [EPR12821] ab175930, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human prostate tissue labeling VPS24 with Anti-VPS24 antibody [EPR12821] ab175930 at 1/50 dilution Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (ab249897), expandable thumbnail](https://content.abcam.com/products/images/vps24-antibody-epr12821-bsa-and-azide-free-ab249897--immunoprecipitation-img73856.jpg "Immunoprecipitation - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (ab249897)")
Immunoprecipitation - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (ab249897)
This data was developed using Anti-VPS24 antibody [EPR12821] ab175930, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from (1) HepG2 cells lysate or (2) 1X PBS (negative control) using Anti-VPS24 antibody [EPR12821] ab175930 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes: Immunoprecipitation - Anti-VPS24 antibody [EPR12821] (Anti-VPS24 antibody [EPR12821] ab175930)
Predicted band size: 25 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (ab249897), expandable thumbnail](https://content.abcam.com/products/images/vps24-antibody-epr12821-bsa-and-azide-free-ab249897--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116687.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (ab249897)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (ab249897)
This data was developed using Anti-VPS24 antibody [EPR12821] ab175930, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue using labeling VPS24 with Anti-VPS24 antibody [EPR12821] ab175930 at 1/50 dilution Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (ab249897), expandable thumbnail](https://content.abcam.com/products/images/vps24-antibody-epr12821-bsa-and-azide-free-ab249897--flow-cytometry-intracellular-img27172.jpg "Flow Cytometry (Intracellular) - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (ab249897)")
Flow Cytometry (Intracellular) - Anti-VPS24 antibody [EPR12821] - BSA and Azide free (ab249897)
This data was developed using Anti-VPS24 antibody [EPR12821] ab175930, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized 293T cells labeling VPS24 with Anti-VPS24 antibody [EPR12821] ab175930 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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