Anti-VPS26 antibody

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody (AB23892)

ab23892 staining VPS26 in U-2 OS cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab23892 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• WB

AbReview31780****

Western blot - Anti-VPS26 antibody (AB23892)

Blocked with 5% milk for 30 min at 25°C

All lanes:

Western blot - Anti-VPS26 antibody (ab23892) at 1/1000 dilution

Lane 1:

HEK293T whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilution

Predicted band size: 38 kDa

Observed band size: 40 kDa

true

Exposure time: 2s

This image is courtesy of an anonymous Abreview

• WB

Lab

Western blot - Anti-VPS26 antibody (AB23892)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : VPS26 knockout HAP1 cell lysate (20 μg)
Lane 3 : Jurkat cell lysate (20 μg)
Lanes 1 - 3 : Merged signal (red and green). Green - ab23892 observed at 40 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab23892 was shown to recognize VPS26 when VPS26 knockout samples were used, along with additional cross-reactive bands. Wild-type and VPS26 knockout samples were subjected to SDS-PAGE. ab23892 at a concentration of 1 μg/ml and ab8245 (loading control to GAPDH) diluted to 1/10000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-VPS26 antibody (ab23892)

Predicted band size: 38 kDa

false

• WB

Project1353****

Western blot - Anti-VPS26 antibody (AB23892)

ab23892 detects a band of ~ 38kDa in Human kidney lysate. This band is quenched by the addition of the immunizing peptide, ab24288. ab23892 also detects a band of the correct size in HEK293 and mouse kidney lysate (data not shown).

All lanes:

Western blot - Anti-VPS26 antibody (ab23892) at 1 µg/mL

Lane 1:

Human kidney lysate at 20 µg

Lane 2:

Human kidney lysate at 20 µg with Human VPS26 peptide (<a href='/en-us/products/unavailable/human-vps26-peptide-ab24288'>ab24288</a>)

Secondary

All lanes:

Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution

Predicted band size: 38 kDa

Observed band size: 25 kDa,37 kDa,50 kDa

false

• WB

AbReview31782****

Western blot - Anti-VPS26 antibody (AB23892)

Blocked with 5% milk for 30 min at 25°C

All lanes:

Western blot - Anti-VPS26 antibody (ab23892) at 1/1000 dilution

Lane 1:

Rat NRK whole cell lysate at 25 µg

Lane 2:

Rat NRK whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilution

Predicted band size: 38 kDa

Observed band size: 40 kDa

true

Exposure time: 10s

This image is courtesy of an anonymous Abreview

• WB

AbReview31783****

Western blot - Anti-VPS26 antibody (AB23892)

All lanes:

Western blot - Anti-VPS26 antibody (ab23892) at 1/1000 dilution

All lanes:

COS whole cell lysate, African Green Monkey at 50 µg

Predicted band size: 38 kDa

true

Exposure time: 10s

This image is courtesy of an anonymous Abreview.