Rabbit Polyclonal VPS26 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 64 publications.
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- Molecular neurodegeneration 18:512023VPS35 and α-Synuclein fail to interact to modulate neurodegeneration in rodent models of Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXi Chen,Elpida Tsika,Nathan Levine,Darren J MoorePubMed 37542299
- Science advances 9:eadd26712023CREB3L2-ATF4 heterodimerization defines a transcriptional hub of Alzheimer's disease gene expression linked to neuropathology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCláudio Gouveia Roque,Kyung Min Chung,Ethan P McCurdy,Radhika Jagannathan,Lisa K Randolph,Krystal Herline-Killian,Jimena Baleriola,Ulrich HengstPubMed 36867706
- Life science alliance 6:2023FAM21 is critical for TLR2/CLEC4E-mediated dendritic cell function against .Applications:Unspecified applicationReactive species:Unspecified reactive speciesRakesh Kulkarni,Siti Khadijah Kasani,Ching-Yen Tsai,Shu-Yun Tung,Kun-Hai Yeh,Chen-Hsin Albert Yu,Wen ChangPubMed 36717248
- Cell reports. Medicine 3:1007402022A genetically modified minipig model for Alzheimer's disease with SORL1 haploinsufficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlav M Andersen,Nikolaj Bøgh,Anne M Landau,Gro G Pløen,Anne Mette G Jensen,Giulia Monti,Benedicte P Ulhøi,Jens R Nyengaard,Kirsten R Jacobsen,Margarita M Jørgensen,Ida E Holm,Marianne L Kristensen,Aage Kristian O Alstrup,Esben S S Hansen,Charlotte E Teunissen,Laura Breidenbach,Mathias Droescher,Ying Liu,Hanne S Pedersen,Henrik Callesen,Yonglun Luo,Lars Bolund,David J Brooks,Christoffer Laustsen,Scott A Small,Lars F Mikkelsen,Charlotte B SørensenPubMed 36099918
- Journal of nanobiotechnology 20:3872022Directing ricin-based immunotoxins with targeting affibodies and KDEL signal peptide to cancer cells effectively induces apoptosis and tumor suppression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeong Guk Park,Heeyeon Kim,Heejin Jun,Sun Young Choi,Eunhee Kim,Sebyung KangPubMed 35999603
- Proceedings of the National Academy of Sciences of the United States of America 119:e21187551192022Retromer dysfunction in amyotrophic lateral sclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEduardo J Pérez-Torres,Irina Utkina-Sosunova,Vartika Mishra,Peter Barbuti,Mariangels De Planell-Saguer,Georgia Dermentzaki,Heather Geiger,Anna O Basile,Nicolas Robine,Delphine Fagegaltier,Kristin A Politi,Paola Rinchetti,Vernice Jackson-Lewis,Matthew Harms,Hemali Phatnani,Francesco Lotti,Serge PrzedborskiPubMed 35749364
- Cell reports 38:1102622022The neuronal retromer can regulate both neuronal and microglial phenotypes of Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYasir H Qureshi,Diego E Berman,Samuel E Marsh,Ronald L Klein,Vivek M Patel,Sabrina Simoes,Suvarnambiga Kannan,Gregory A Petsko,Beth Stevens,Scott A SmallPubMed 35045281
- Cell reports 37:1101822021Alzheimer's vulnerable brain region relies on a distinct retromer core dedicated to endosomal recycling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSabrina Simoes,Jia Guo,Luna Buitrago,Yasir H Qureshi,Xinyang Feng,Milankumar Kothiya,Etty Cortes,Vivek Patel,Suvarnambiga Kannan,Young-Hyun Kim,Kyu-Tae Chang,S Abid Hussaini,Herman Moreno,Gilbert Di Paolo,Olav M Andersen,Scott A SmallPubMed 34965419
- Science advances 7:eabg40072021De novo macrocyclic peptides for inhibiting, stabilizing, and probing the function of the retromer endosomal trafficking complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai-En Chen,Qian Guo,Timothy A Hill,Yi Cui,Amy K Kendall,Zhe Yang,Ryan J Hall,Michael D Healy,Joanna Sacharz,Suzanne J Norwood,Sachini Fonseka,Boyang Xie,Robert C Reid,Natalya Leneva,Robert G Parton,Rajesh Ghai,David A Stroud,David P Fairlie,Hiroaki Suga,Lauren P Jackson,Rohan D Teasdale,Toby Passioura,Brett M CollinsPubMed 34851660
- Brain communications 3:fcab2082021Neuronal deletion induces spinal cord motor neuron degeneration and early post-natal lethality.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDorian Sargent,Lindsey A Cunningham,Dylan J Dues,Yue Ma,Jennifer J Kordich,Gabriela Mercado,Patrik Brundin,Rita M Cowell,Darren J MoorePubMed 34704029
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Expected |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Abcam recommends using milk as the blocking agent. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes Abcam recommends using milk as the blocking agent. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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4 products for Alternative Product
Target data
Function
Acts as a component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins (Probable). The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R (PubMed:15078902, PubMed:15078903). Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) (PubMed:15247922). Required for the endosomal localization of WASHC2A (indicative for the WASH complex) (PubMed:22070227). Required for the endosomal localization of TBC1D5 (PubMed:20923837). Mediates retromer cargo recognition of SORL1 and is involved in trafficking of SORL1 implicated in sorting and processing of APP (PubMed:22279231). Involved in retromer-independent lysosomal sorting of F2R (PubMed:16407403). Involved in recycling of ADRB2 (PubMed:21602791). Enhances the affinity of SNX27 for PDZ-binding motifs in cargo proteins (By similarity).
Alternative names
VPS26, VPS26A, Vacuolar protein sorting-associated protein 26A, Vesicle protein sorting 26A, hVPS26
Recommended products
Rabbit Polyclonal VPS26 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 64 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Replenishment batches of our polyclonal antibody, ab23892 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab181352.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
Western blot - Anti-VPS26 antibody (ab23892)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: VPS26 knockout HAP1 cell lysate (20 μg)
Lane 3: Jurkat cell lysate (20 μg)
Lanes 1 - 3: Merged signal (red and green). Green - ab23892 observed at 40 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab23892 was shown to recognize VPS26 when VPS26 knockout samples were used, along with additional cross-reactive bands. Wild-type and VPS26 knockout samples were subjected to SDS-PAGE. ab23892 at a concentration of 1 μg/ml and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) diluted to 1/10000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-VPS26 antibody (ab23892)
Predicted band size: 38 kDa
Western blot - Anti-VPS26 antibody (ab23892)
ab23892 detects a band of ~ 38kDa in Human kidney lysate. This band is quenched by the addition of the immunizing peptide, ab24288. ab23892 also detects a band of the correct size in HEK293 and mouse kidney lysate (data not shown).
All lanes: Western blot - Anti-VPS26 antibody (ab23892) at 1 µg/mL
Lane 1: Human kidney lysate at 20 µg
Lane 2: Human kidney lysate at 20 µg with Human VPS26 peptide (ab24288)
SecondaryAll lanes: Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 25 kDa, 37 kDa, 50 kDa
This image is courtesy of an anonymous customer reviewWestern blot - Anti-VPS26 antibody (ab23892)
Blocked with 5% milk for 30 min at 25°C
All lanes: Western blot - Anti-VPS26 antibody (ab23892) at 1/1000 dilution
Lane 1: Rat NRK whole cell lysate at 25 µg
Lane 2: Rat NRK whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 40 kDa
Exposure time: 10s
This image is courtesy of an anonymous customer reviewWestern blot - Anti-VPS26 antibody (ab23892)
Blocked with 5% milk for 30 min at 25°C
All lanes: Western blot - Anti-VPS26 antibody (ab23892) at 1/1000 dilution
Lane 1: HEK293T whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 40 kDa
Exposure time: 2s
This image is courtesy of an anonymous customer review.Western blot - Anti-VPS26 antibody (ab23892)
All lanes: Western blot - Anti-VPS26 antibody (ab23892) at 1/1000 dilution
All lanes: COS whole cell lysate, African Green Monkey at 50 µg with Milk, 30 minutes, 25°C
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Exposure time: 10s
Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody (ab23892)
ab23892 staining VPS26 in U-2 OS cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab23892 at 5µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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