JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB211530

Anti-VPS26 antibody [CL2287] - C-terminal

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Monoclonal VPS26 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human VPS26A aa 300-350.

View Alternative Names

VPS26, VPS26A, Vacuolar protein sorting-associated protein 26A, Vesicle protein sorting 26A, hVPS26

11 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling VPS26 with ab211530 at 1/200 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)

Immunofluorescent analysis of MCF7 cells (PFA-fixed/Triton X-100 permeabilized) labeling VPS26 with ab211530 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.

Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)

Immunofluorescent analysis of U251 cells (PFA-fixed/Triton X-100 permeabilized) labeling VPS26 with ab211530 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.

Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)

Immunofluorescent analysis of U2OS cells (PFA-fixed/Triton X-100 permeabilized) labeling VPS26 with ab211530 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.

Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)

Immunofluorescent analysis of A431 cells (PFA-fixed/Triton X-100 permeabilized) labeling VPS26 with ab211530 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)

Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling VPS26 with ab211530 at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)

Immunohistochemical analysis of paraffin-embedded Human fallopian tube tissue labeling VPS26 with ab211530 at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling VPS26 with ab211530 at 1/200 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)

Immunofluorescent analysis of HeLa cells (PFA-fixed/Triton X-100 permeabilized) labeling VPS26 with ab211530 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.

Western blot - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)
  • WB

Supplier Data

Western blot - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)

All lanes:

Western blot - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530) at 1/200 dilution

All lanes:

U251 MG cell line lysate

Predicted band size: 38 kDa

false

Western blot - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)
  • WB

Supplier Data

Western blot - Anti-VPS26 antibody [CL2287] - C-terminal (AB211530)

Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.

All lanes:

Western blot - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)

Lane 1:

U-251 cells, transfected with control siRNA

Lane 2:

U-251 cells, transfected with target specific siRNA probe #1

Lane 3:

U-251 cells, transfected with target specific siRNA probe #2

Predicted band size: 38 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL2287

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human VPS26A aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

O75436

style
left-column

Alternative Products

Primary Antibodies

AB23892

Anti-VPS26 antibody

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody (AB23892)

  • 4
  • 12
Primary Antibodies

AB180172

Anti-VPS26 antibody [EPR13455(B)] - N-terminal

RabMAb|Recombinant|KO Validated

Western blot - Anti-VPS26 antibody [EPR13455(B)] - N-terminal (AB180172)

  • 0
  • 0
Primary Antibodies

AB181352

Anti-VPS26 antibody [EPR13456]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [EPR13456] (AB181352)

  • 4
  • 1

Complementary Products

Primary Antibodies

AB157220

Anti-VPS35 antibody [EPR11501(B)]

KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-VPS35 antibody [EPR11501(B)] (AB157220)

  • 2
  • 1
Primary Antibodies

AB2900

Anti-EEA1 antibody - Early Endosome Marker

KO Validated|Lab Essentials

Western blot - Anti-EEA1 antibody - Early Endosome Marker (AB2900)

  • 4
  • 39
Primary Antibodies

AB125025

Anti-ROCK2 antibody [EPR7141(B)]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 antibody [EPR7141(B)] (AB125025)

  • 4
  • 4
Primary Antibodies

AB115730

Anti-Glucose Transporter GLUT1 antibody [EPR3915]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB115730)

  • 4
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Acts as a component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins (Probable). The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R (PubMed : 15078902, PubMed : 15078903). Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) (PubMed : 15247922). Required for the endosomal localization of WASHC2A (indicative for the WASH complex) (PubMed : 22070227). Required for the endosomal localization of TBC1D5 (PubMed : 20923837). Mediates retromer cargo recognition of SORL1 and is involved in trafficking of SORL1 implicated in sorting and processing of APP (PubMed : 22279231). Involved in retromer-independent lysosomal sorting of F2R (PubMed : 16407403). Involved in recycling of ADRB2 (PubMed : 21602791). Enhances the affinity of SNX27 for PDZ-binding motifs in cargo proteins (By similarity).
See full target information VPS26A
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:342 PubMed40280967

2025

Targeting metabolic vulnerability by combining NAMPT inhibitors and disulfiram for treatment of recurrent ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kei Kudo,Yoshimi Endo Greer,Daniel R Crooks,Ye Yang,Jeffrey R Brender,Teruhiko Yoshida,Brittney S Harrington,Rahul Kamdar,Soumya Korrapati,Yusuke Shibuya,Leah Henegar,Jeffrey Kopp,Takeo Fujii,Stanley Lipkowitz,Christina M Annunziata
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com