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AB181352

Anti-VPS26 antibody [EPR13456]

4

(1 Review)

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(17 Publications)

Rabbit Recombinant Monoclonal VPS26 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 17 publications.

View Alternative Names

VPS26, VPS26A, Vacuolar protein sorting-associated protein 26A, Vesicle protein sorting 26A, hVPS26

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [EPR13456] (AB181352)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [EPR13456] (AB181352)

Immunofluorescent analysis of HeLa cells labeling VPS26 with ab181352 at 1/100 dilution (red). DAPI nuclear staining (blue).

Immunoprecipitation - Anti-VPS26 antibody [EPR13456] (AB181352)
  • IP

Supplier Data

Immunoprecipitation - Anti-VPS26 antibody [EPR13456] (AB181352)

Western blot analysis on immunoprecipitation pellet from HeLa cell lysate pulling down VPS26 using ab181352 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-VPS26 antibody [EPR13456] (ab181352)

Predicted band size: 38 kDa

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Western blot - Anti-VPS26 antibody [EPR13456] (AB181352)
  • WB

Supplier Data

Western blot - Anti-VPS26 antibody [EPR13456] (AB181352)

All lanes:

Western blot - Anti-VPS26 antibody [EPR13456] (ab181352) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Predicted band size: 38 kDa

false

Western blot - Anti-VPS26 antibody [EPR13456] (AB181352)
  • WB

Lab

Western blot - Anti-VPS26 antibody [EPR13456] (AB181352)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : VPS26 knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : HepG2 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab181352 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.

ab181352 was shown to specifically react with VPS26 when VPS26 knockout samples were used. Wild-type and VPS26 knockout samples were subjected to SDS-PAGE. ab181352 and ab18058 (loading control to Vinculin) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ( ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-VPS26 antibody [EPR13456] (ab181352)

Predicted band size: 38 kDa

false

  • Carrier free

    Anti-VPS26 antibody [EPR13456] - BSA and Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-VPS26 antibody [EPR13456]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-VPS26 antibody [EPR13456]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-VPS26 antibody [EPR13456]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-VPS26 antibody [EPR13456]

  • 578 PE

    PE Anti-VPS26 antibody [EPR13456]

  • 660 APC

    APC Anti-VPS26 antibody [EPR13456]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13456

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins (Probable). The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R (PubMed : 15078902, PubMed : 15078903). Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) (PubMed : 15247922). Required for the endosomal localization of WASHC2A (indicative for the WASH complex) (PubMed : 22070227). Required for the endosomal localization of TBC1D5 (PubMed : 20923837). Mediates retromer cargo recognition of SORL1 and is involved in trafficking of SORL1 implicated in sorting and processing of APP (PubMed : 22279231). Involved in retromer-independent lysosomal sorting of F2R (PubMed : 16407403). Involved in recycling of ADRB2 (PubMed : 21602791). Enhances the affinity of SNX27 for PDZ-binding motifs in cargo proteins (By similarity).
See full target information VPS26A
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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed39273021

2024

Cell-Sonar, a Novel Method for Intracellular Tracking of Secretory Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Brockmöller,Thomas Seeger,Franz Worek,Simone Rothmiller

Autophagy 20:863-882 PubMed37938196

2023

Autophagy captures the retromer-TBC1D5 complex to inhibit receptor recycling.

Applications

Unspecified application

Species

Unspecified reactive species

Julian M Carosi,Leanne K Hein,Jarrod J Sandow,Linh V P Dang,Kathryn Hattersley,Donna Denton,Sharad Kumar,Timothy J Sargeant

Carcinogenesis 43:1059-1070 PubMed36070764

2022

LAMTOR1 degrades MHC-II via the endocytic in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Wu,Qian Wang,Bowen Li,Meixi Jiang

The EMBO journal 41:e109646 PubMed35466426

2022

CROP: a retromer-PROPPIN complex mediating membrane fission in the endo-lysosomal system.

Applications

Unspecified application

Species

Unspecified reactive species

Thibault Courtellemont,Maria Giovanna De Leo,Navin Gopaldass,Andreas Mayer

Frontiers in immunology 12:814570 PubMed35095913

2022

Sorting Nexin 27 Enables MTOC and Secretory Machinery Translocation to the Immune Synapse.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia González-Mancha,Cristina Rodríguez-Rodríguez,Andrés Alcover,Isabel Merida

Cell reports 36:109338 PubMed34233182

2021

Regulation of NMDA receptor trafficking and gating by activity-dependent CaMKIIα phosphorylation of the GluN2A subunit.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Ling Hilary Yong,Lingrui Zhang,Liming Yang,Xiumin Chen,Jing Zhi Anson Tan,Xiaojun Yu,Mintu Chandra,Emma Livingstone,Jocelyn Widagdo,Marta M Vieira,Katherine W Roche,Joseph W Lynch,Angelo Keramidas,Brett M Collins,Victor Anggono

Autophagy 17:2217-2237 PubMed32960680

2020

Retromer regulates the lysosomal clearance of MAPT/tau.

Applications

Unspecified application

Species

Unspecified reactive species

Julian M Carosi,Leanne K Hein,Mark van den Hurk,Robert Adams,Bridget Milky,Sanjna Singh,Cedric Bardy,Donna Denton,Sharad Kumar,Timothy J Sargeant

Cell reports 29:2599-2607.e6 PubMed31775031

2019

SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Els F Halff,Blanka R Szulc,Flavie Lesept,Josef T Kittler

Nature communications 10:1454 PubMed30926795

2019

USP32 regulates late endosomal transport and recycling through deubiquitylation of Rab7.

Applications

Unspecified application

Species

Unspecified reactive species

Aysegul Sapmaz,Ilana Berlin,Erik Bos,Ruud H Wijdeven,Hans Janssen,Rebecca Konietzny,Jimmy J Akkermans,Ayse E Erson-Bensan,Roman I Koning,Benedikt M Kessler,Jacques Neefjes,Huib Ovaa

The Biochemical journal 475:1861-1883 PubMed29743203

2018

The Parkinson's disease VPS35[D620N] mutation enhances LRRK2-mediated Rab protein phosphorylation in mouse and human.

Applications

Unspecified application

Species

Unspecified reactive species

Rafeeq Mir,Francesca Tonelli,Pawel Lis,Thomas Macartney,Nicole K Polinski,Terina N Martinez,Meng-Yun Chou,Andrew J M Howden,Theresa König,Christoph Hotzy,Ivan Milenkovic,Thomas Brücke,Alexander Zimprich,Esther Sammler,Dario R Alessi
View all publications
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