Rabbit Recombinant Monoclonal VPS28 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Images
![Western blot - Anti-VPS28 antibody [EPR9880] (AB154793), expandable thumbnail](https://content.abcam.com/products/images/vps28-antibody-epr9880-ab154793--western-blot-img43027.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VPS28 antibody [EPR9880] (AB154793)")
![Western blot - Anti-VPS28 antibody [EPR9880] (AB154793), expandable thumbnail](https://content.abcam.com/products/images/vps28-antibody-epr9880-ab154793--western-blot-img78548.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VPS28 antibody [EPR9880] (AB154793)")
![Western blot - Anti-VPS28 antibody [EPR9880] (AB154793), expandable thumbnail](https://content.abcam.com/products/images/vps28-antibody-epr9880-ab154793--western-blot-img65024.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VPS28 antibody [EPR9880] (AB154793)")
![Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (AB154793), expandable thumbnail](https://content.abcam.com/products/images/vps28-antibody-epr9880-ab154793--immunoprecipitation-img102564.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (AB154793)")
![Western blot - Anti-VPS28 antibody [EPR9880] (AB154793), expandable thumbnail](https://content.abcam.com/products/images/vps28-antibody-epr9880-ab154793--western-blot-img153195.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VPS28 antibody [EPR9880] (AB154793)")
Publications
Filter by
- Frontiers in molecular biosciences 8:6341832021Deciphering the Oncogenic Role of VPS28 Modulated by miR-491-5p in Breast Cancer Cells Using In Silico and Functional Analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjie Shi,Daojun Hu,Yu Xing,Rui Zhuo,Qiufeng Lao,Hui Liu,Weiyi PangPubMed 34395516
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Not recommended |
| Mouse | Expected | Tested | Not recommended |
| Rat | Expected | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Component of the ESCRT-I complex, a regulator of vesicular trafficking process.
Alternative names
Vacuolar protein sorting-associated protein 28 homolog, H-Vps28, ESCRT-I complex subunit VPS28, VPS28
Recommended products
Rabbit Recombinant Monoclonal VPS28 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR9880
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-VPS28 antibody [EPR9880] (ab154793), expandable thumbnail](https://content.abcam.com/products/images/vps28-antibody-epr9880-ab154793--western-blot-img43027.jpg "Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)")
Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-VPS28 antibody [EPR9880] (ab154793) at 1/2000 dilution
All lanes: HepG2 cell lysate at 10 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
![Western blot - Anti-VPS28 antibody [EPR9880] (ab154793), expandable thumbnail](https://content.abcam.com/products/images/vps28-antibody-epr9880-ab154793--western-blot-img78548.jpg "Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)")
Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1500 dilution
All lanes: HepG2 cell lysate at 10 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
![Western blot - Anti-VPS28 antibody [EPR9880] (ab154793), expandable thumbnail](https://content.abcam.com/products/images/vps28-antibody-epr9880-ab154793--western-blot-img65024.jpg "Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)")
Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-VPS28 antibody [EPR9880] (ab154793) at 1/2000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Rat brain tissue lysate at 20 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 116 kDa, 119 kDa, 25 kDa, 27 kDa, 32 kDa, 34 kDa, 36 kDa, 57 kDa, 59 kDa
Observed band size: 116 kDa, 25 kDa, 36 kDa, 40 kDa, 57 kDa, 63 kDa, 87 kDa
![Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793), expandable thumbnail](https://content.abcam.com/products/images/vps28-antibody-epr9880-ab154793--immunoprecipitation-img102564.jpg "Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)")
Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)
ab154793 (unpurified) at 1/50 immunoprecipitating VPS28 in jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)
Predicted band size: 25 kDa
Observed band size: 25 kDa
![Western blot - Anti-VPS28 antibody [EPR9880] (ab154793), expandable thumbnail](https://content.abcam.com/products/images/vps28-antibody-epr9880-ab154793--western-blot-img153195.jpg "Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)")
Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1500 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Rat brain tissue lysate at 20 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
![Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793), expandable thumbnail](https://content.abcam.com/products/images/vps28-antibody-epr9880-ab154793--immunoprecipitation-img102565.jpg "Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)")
Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)
ab154793 (purified) at 1/50 immunoprecipitating VPS28 in jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)
Predicted band size: 25 kDa
Observed band size: 25 kDa
![Western blot - Anti-VPS28 antibody [EPR9880] (ab154793), expandable thumbnail](https://content.abcam.com/products/images/vps28-antibody-epr9880-ab154793--western-blot-img101211.jpg "Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)")
Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
All lanes: Western blot - Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: LnCaP cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 25 kDa
![Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793), expandable thumbnail](https://content.abcam.com/products/images/vps28-antibody-epr9880-ab154793--immunoprecipitation-img102565.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)")
![Western blot - Anti-VPS28 antibody [EPR9880] (ab154793), expandable thumbnail](https://content.abcam.com/products/images/vps28-antibody-epr9880-ab154793--western-blot-img101211.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)")
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