AB154793

Anti-VPS28 antibody [EPR9880]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal VPS28 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

Vacuolar protein sorting-associated protein 28 homolog, H-Vps28, ESCRT-I complex subunit VPS28, VPS28

7 Images

Lab

Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (AB154793)

ab154793 (unpurified) at 1/50 immunoprecipitating VPS28 in jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Lab

Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (AB154793)

ab154793 (purified) at 1/50 immunoprecipitating VPS28 in jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Unknown

Western blot - Anti-VPS28 antibody [EPR9880] (AB154793)

All lanes:

Western blot - Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

LnCaP cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 25 kDa

false

Lab

Western blot - Anti-VPS28 antibody [EPR9880] (AB154793)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-VPS28 antibody [EPR9880] (ab154793) at 1/2000 dilution

All lanes:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Lab

Western blot - Anti-VPS28 antibody [EPR9880] (AB154793)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1500 dilution

All lanes:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Lab

Western blot - Anti-VPS28 antibody [EPR9880] (AB154793)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-VPS28 antibody [EPR9880] (ab154793) at 1/2000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 116 kDa,119 kDa,25 kDa,27 kDa,32 kDa,34 kDa,36 kDa,57 kDa,59 kDa

Observed band size: 116 kDa,25 kDa,36 kDa,40 kDa,57 kDa,63 kDa,87 kDa

false

Lab

Western blot - Anti-VPS28 antibody [EPR9880] (AB154793)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1500 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Carrier free

Anti-VPS28 antibody [EPR9880] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9880

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the ESCRT-I complex, a regulator of vesicular trafficking process.

See full target information VPS28

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 102:105053 PubMed38471398

2024

High lymphocyte signature genes expression in parathyroid endocrine cells and its downregulation linked to tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Geng,Junjun Liu,Bingzhou Guo,Kailin Liu,Pengfei Gong,Bao Wang,Qiang Wan,Liang Sun,Jiajun Zhao,Yongfeng Song

Frontiers in molecular biosciences 8:634183 PubMed34395516

2021

Deciphering the Oncogenic Role of VPS28 Modulated by miR-491-5p in Breast Cancer Cells Using In Silico and Functional Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjie Shi,Daojun Hu,Yu Xing,Rui Zhuo,Qiufeng Lao,Hui Liu,Weiyi Pang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VPS28 antibody [EPR9880]

Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (AB154793)

Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (AB154793)

Western blot - Anti-VPS28 antibody [EPR9880] (AB154793)

Western blot - Anti-VPS28 antibody [EPR9880] (AB154793)

Western blot - Anti-VPS28 antibody [EPR9880] (AB154793)

Western blot - Anti-VPS28 antibody [EPR9880] (AB154793)

Western blot - Anti-VPS28 antibody [EPR9880] (AB154793)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise