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AB249102

Anti-VPS28 antibody [EPR9880] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal VPS28 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Vacuolar protein sorting-associated protein 28 homolog, H-Vps28, ESCRT-I complex subunit VPS28, VPS28

7 Images
Immunoprecipitation - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (AB249102)
  • IP

Lab

Immunoprecipitation - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (AB249102)

This data was developed using ab154793, the same antibody clone in a different buffer formulation.

ab154793 (unpurified) at 1/50 immunoprecipitating VPS28 in jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (<a href='/en-us/products/primary-antibodies/vps28-antibody-epr9880-ab154793'>ab154793</a>)

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Immunoprecipitation - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (AB249102)
  • IP

Lab

Immunoprecipitation - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (AB249102)

This data was developed using ab154793, the same antibody clone in a different buffer formulation.

ab154793 (purified) at 1/50 immunoprecipitating VPS28 in jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (<a href='/en-us/products/primary-antibodies/vps28-antibody-epr9880-ab154793'>ab154793</a>)

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Western blot - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (AB249102)
  • WB

Unknown

Western blot - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (AB249102)

This data was developed using ab154793, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-VPS28 antibody [EPR9880] (<a href='/en-us/products/primary-antibodies/vps28-antibody-epr9880-ab154793'>ab154793</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

LnCaP cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 25 kDa

false

Western blot - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (AB249102)
  • WB

Lab

Western blot - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (AB249102)

This data was developed using ab154793, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-VPS28 antibody [EPR9880] (<a href='/en-us/products/primary-antibodies/vps28-antibody-epr9880-ab154793'>ab154793</a>) at 1/2000 dilution

All lanes:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Western blot - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (AB249102)
  • WB

Lab

Western blot - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (AB249102)

This data was developed using ab154793, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-VPS28 antibody [EPR9880] (<a href='/en-us/products/primary-antibodies/vps28-antibody-epr9880-ab154793'>ab154793</a>) at 1/1500 dilution

All lanes:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Western blot - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (AB249102)
  • WB

Lab

Western blot - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (AB249102)

This data was developed using ab154793, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-VPS28 antibody [EPR9880] (<a href='/en-us/products/primary-antibodies/vps28-antibody-epr9880-ab154793'>ab154793</a>) at 1/2000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 116 kDa,119 kDa,25 kDa,27 kDa,32 kDa,34 kDa,36 kDa,57 kDa,59 kDa

Observed band size: 116 kDa,25 kDa,36 kDa,40 kDa,57 kDa,63 kDa,87 kDa

false

Western blot - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (AB249102)
  • WB

Lab

Western blot - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (AB249102)

This data was developed using ab154793, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-VPS28 antibody [EPR9880] (<a href='/en-us/products/primary-antibodies/vps28-antibody-epr9880-ab154793'>ab154793</a>) at 1/1500 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9880

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 16
Primary Antibodies

AB251760

Anti-VPS37A/HCRP1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-VPS37A/HCRP1 antibody (AB251760)

  • 0
  • 0
Primary Antibodies

AB185548

Anti-ABCE1 antibody [EPR15373(B)] - C-terminal

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (AB185548)

  • 0
  • 0
Primary Antibodies

AB7780

Anti-Ubiquitin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin antibody (AB7780)

  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab249102 is the carrier-free version of ab154793.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the ESCRT-I complex, a regulator of vesicular trafficking process.
See full target information VPS28
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 60:2744-2760.e9 PubMed40460835

2025

ATG9A and ARFIP2 cooperate to control PI4P levels for lysosomal repair.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano De Tito,Eugenia Almacellas,Daniel Dai Yu,Emily Millard,Wenxin Zhang,Cecilia de Heus,Christophe Queval,Javier H Hervás,Enrica Pellegrino,Ioanna Panagi,Ditte Fogde,Teresa L M Thurston,Judith Klumperman,Maximiliano Gutierrez,Sharon A Tooze
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com