Goat Polyclonal VPS35 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 68 publications. Immunogen corresponding to Synthetic Peptide within Human VPS35 aa 750 to C-terminus.
Images
Publications
Filter by
- Frontiers in cell and developmental biology 11:12405582023A missense mutation in associated with defective spermatogenesis and male infertility.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatrin Meindl,Naomi Issler,Sara Afonso,Alberto Cebrian-Serrano,Karin Müller,Christina Sterner,Helga Othmen,Ines Tegtmeier,Ralph Witzgall,Enriko Klootwijk,Benjamin Davies,Robert Kleta,Richard WarthPubMed 37900275
- Nature communications 14:39112023Loss of the batten disease protein CLN3 leads to mis-trafficking of M6PR and defective autophagic-lysosomal reformation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlessia Calcagni',Leopoldo Staiano,Nicolina Zampelli,Nadia Minopoli,Niculin J Herz,Giuseppe Di Tullio,Tuong Huynh,Jlenia Monfregola,Alessandra Esposito,Carmine Cirillo,Aleksandar Bajic,Mahla Zahabiyon,Rachel Curnock,Elena Polishchuk,Luke Parkitny,Diego Luis Medina,Nunzia Pastore,Peter J Cullen,Giancarlo Parenti,Maria Antonietta De Matteis,Paolo Grumati,Andrea BallabioPubMed 37400440
- Nature communications 14:28472023Phospholipase D3 degrades mitochondrial DNA to regulate nucleotide signaling and APP metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZoë P Van Acker,Anika Perdok,Ruben Hellemans,Katherine North,Inge Vorsters,Cedric Cappel,Jonas Dehairs,Johannes V Swinnen,Ragna Sannerud,Marine Bretou,Markus Damme,Wim AnnaertPubMed 37225734
- Cell 186:2219-2237.e292023Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael D Healy,Kerrie E McNally,Rebeka Butkovič,Molly Chilton,Kohji Kato,Joanna Sacharz,Calum McConville,Edmund R R Moody,Shrestha Shaw,Vicente J Planelles-Herrero,Sathish K N Yadav,Jennifer Ross,Ufuk Borucu,Catherine S Palmer,Kai-En Chen,Tristan I Croll,Ryan J Hall,Nikeisha J Caruana,Rajesh Ghai,Thi H D Nguyen,Kate J Heesom,Shinji Saitoh,Imre Berger,Christiane Schaffitzel,Tom A Williams,David A Stroud,Emmanuel Derivery,Brett M Collins,Peter J CullenPubMed 37172566
- Science advances 9:eadd26712023CREB3L2-ATF4 heterodimerization defines a transcriptional hub of Alzheimer's disease gene expression linked to neuropathology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCláudio Gouveia Roque,Kyung Min Chung,Ethan P McCurdy,Radhika Jagannathan,Lisa K Randolph,Krystal Herline-Killian,Jimena Baleriola,Ulrich HengstPubMed 36867706
- iScience 26:1061502023Lysosomal trafficking of the glucose transporter GLUT1 requires sequential regulation by TXNIP and ubiquitin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSusan J Qualls-Histed,Casey P Nielsen,Jason A MacGurnPubMed 36890792
- The EMBO journal 42:e1122872023Retromer oligomerization drives SNX-BAR coat assembly and membrane constriction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNavin Gopaldass,Maria Giovanna De Leo,Thibault Courtellemont,Vincent Mercier,Christin Bissig,Aurélien Roux,Andreas MayerPubMed 36644906
- eLife 12:2023Exploring therapeutic strategies for infantile neuronal axonal dystrophy (INAD/PARK14).Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuang Lin,Burak Tepe,Geoff McGrane,Regine C Tipon,Gist Croft,Leena Panwala,Amanda Hope,Agnes J H Liang,Zhongyuan Zuo,Seul Kee Byeon,Lily Wang,Akhilesh Pandey,Hugo J BellenPubMed 36645408
- Cell reports 41:1116532022USP32-regulated LAMTOR1 ubiquitination impacts mTORC1 activation and autophagy induction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandra Hertel,Ludovico Martins Alves,Henrik Dutz,Georg Tascher,Florian Bonn,Manuel Kaulich,Ivan Dikic,Stefan Eimer,Florian Steinberg,Anja BremmPubMed 36476874
- Proceedings of the National Academy of Sciences of the United States of America 119:e21187551192022Retromer dysfunction in amyotrophic lateral sclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEduardo J Pérez-Torres,Irina Utkina-Sosunova,Vartika Mishra,Peter Barbuti,Mariangels De Planell-Saguer,Georgia Dermentzaki,Heather Geiger,Anna O Basile,Nicolas Robine,Delphine Fagegaltier,Kristin A Politi,Paola Rinchetti,Vernice Jackson-Lewis,Matthew Harms,Hemali Phatnani,Francesco Lotti,Serge PrzedborskiPubMed 35749364
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human VPS35 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96QK1
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5.00000-8.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.03000-3.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Human | Dilution info 0.03000-3.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Acts as a component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35; however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway (PubMed:30213940). The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) (PubMed:15078903, PubMed:15247922, PubMed:20164305). Required for endosomal localization of WASHC2C (PubMed:22070227, PubMed:28892079). Mediates the association of the CSC with the WASH complex via WASHC2 (PubMed:22070227, PubMed:24819384, PubMed:24980502). Required for the endosomal localization of TBC1D5 (PubMed:20923837). (Microbial infection) The heterotrimeric retromer cargo-selective complex (CSC) mediates the exit of human papillomavirus from the early endosome and the delivery to the Golgi apparatus.
Alternative names
MEM3, TCCCTA00141, VPS35, Vacuolar protein sorting-associated protein 35, hVPS35, Maternal-embryonic 3, Vesicle protein sorting 35
Recommended products
Goat Polyclonal VPS35 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 68 publications. Immunogen corresponding to Synthetic Peptide within Human VPS35 aa 750 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human VPS35 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96QK1
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
The maintenance of organelles in eukaryotic cells depends on sorting proteins, which ensure the proper delivery of organelle-specific proteins. In S. cerevisiae, VPS35 has a direct role in the retrieval of vacuolar cargo proteins, suggesting that human VPS35 may have an analogous function in the maintenance of lysosomes.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
This image is courtesy of an anonymous customer reviewImmunocytochemistry/ Immunofluorescence - Anti-VPS35 antibody (ab10099)
Immunofluorescence analysis of HeLa cells, staining VPS35 with ab10099.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/150 in 10% Serum in PBS) for 16 hours at 4°C. An AlexaFluor®488-conjugated donkey anti-goat polyclonal IgG (1/300) was used as the secondary antibody.
Western blot - Anti-VPS35 antibody (ab10099)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: VPS35 knockout HAP1 cell lysate (20 μg)
Lane 3: NIH3T3 cell lysate (20 μg)
Lane 4: A549 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab10099 observed at 85 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.
ab10099 was shown to specifically react with VPS35 when VPS35 knockout samples were used. Wild-type and VPS35 knockout samples were subjected to SDS-PAGE. ab10099 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (loading control to GAPDH) were diluted to 1 μg/ml and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with IRDye® 800CW Donkey anti-Goat IgG (H + L) and Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216779) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.All lanes: Western blot - Anti-VPS35 antibody (ab10099)
Predicted band size: 92 kDa
Western blot - Anti-VPS35 antibody (ab10099)
All lanes: Western blot - Anti-VPS35 antibody (ab10099) at 1 µg/mL
Lane 1: Human Cerebellum at 35 µg
Lane 2: Mouse brain at 35 µg
Predicted band size: 92 kDa
Western blot - Anti-VPS35 antibody (ab10099)
Detected by chemiluminescence.
All lanes: Western blot - Anti-VPS35 antibody (ab10099) at 0.03 µg/mL
Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate (35µg protein in RIPA buffer).
Lane 2: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate(35µg protein in RIPA buffer).
Predicted band size: 92 kDa
Immunocytochemistry/ Immunofluorescence - Anti-VPS35 antibody (ab10099)
Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS35 antibody (ab10099)
Immunohistochemical analysis of paraffin embedded human prostate tissue labeling VPS35 with ab10099 at 8 ug/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS35 antibody (ab10099)
Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-VPS35 antibody (ab10099)
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com