Anti-VPS35 antibody [HL1017]

Rabbit Recombinant Monoclonal VPS35 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat, Cat, Dog, Zebrafish samples. Immunogen corresponding to Synthetic Peptide within Human VPS35.

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Images

Western blot - Anti-VPS35 antibody [HL1017] (AB308334), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

Synthetic Peptide within Human VPS35. The exact immunogen used to generate this antibody is proprietary information. Database link Q96QK1

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC/IF	WB
Human	Tested	Tested	Tested
Mouse	Tested	Expected	Tested
Rat	Tested	Expected	Tested
Cat	Expected	Expected	Tested
Dog	Expected	Expected	Tested
Zebrafish	Expected	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50.00000 - 1/100.00000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/50.00000 - 1/100.00000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/50.00000 - 1/100.00000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Cat, Dog, Zebrafish	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Cat, Dog, Zebrafish	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Cat	Dilution info 1/1000	Notes -
Species Dog	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Zebrafish	Dilution info 1/1000	Notes -

Target data

See full target information VPS35

Function

Acts as a component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35; however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway (PubMed:30213940). The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) (PubMed:15078903, PubMed:15247922, PubMed:20164305). Required for endosomal localization of WASHC2C (PubMed:22070227, PubMed:28892079). Mediates the association of the CSC with the WASH complex via WASHC2 (PubMed:22070227, PubMed:24819384, PubMed:24980502). Required for the endosomal localization of TBC1D5 (PubMed:20923837). (Microbial infection) The heterotrimeric retromer cargo-selective complex (CSC) mediates the exit of human papillomavirus from the early endosome and the delivery to the Golgi apparatus.

Alternative names

MEM3, TCCCTA00141, VPS35, Vacuolar protein sorting-associated protein 35, hVPS35, Maternal-embryonic 3, Vesicle protein sorting 35

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: Synthetic Peptide within Human VPS35. The exact immunogen used to generate this antibody is proprietary information. Database link Q96QK1
Carrier free: Yes
Clone number: HL1017
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-VPS35 antibody [HL1017] (ab308334)
Samples were seperated by 7.5% SDS-PAGE.
All lanes: Western blot - Anti-VPS35 antibody [HL1017] (ab308334) at 1/1000 dilution
Lane 1: Non-transfected (–) HeLa whole cell extracts at 30 µg
Lane 2: Transfected (+) HeLa whole cell extracts at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 91 kDa
Western blot - Anti-VPS35 antibody [HL1017] (ab308334)
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (ab308334) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-VPS35 antibody [HL1017] (ab308334) at 1/1000 dilution
Lane 1: Mouse brain lysate at 50 µg
Lane 2: Rat brain lysate at 50 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 91.7 kDa
Western blot - Anti-VPS35 antibody [HL1017] (ab308334)
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (ab308334) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody
All lanes: Western blot - Anti-VPS35 antibody [HL1017] (ab308334) at 1/1000 dilution
Lane 1: A549 cell lysate at 30 µg
Lane 2: H1299 at 30 µg
Lane 3: HCT116 at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 91.7 kDa
Western blot - Anti-VPS35 antibody [HL1017] (ab308334)
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (ab308334) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-VPS35 antibody [HL1017] (ab308334) at 1/1000 dilution
Lane 1: MDCK cell lysate at 30 µg
Lane 2: PG-4 cell lysate at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 91.7 kDa
Western blot - Anti-VPS35 antibody [HL1017] (ab308334)
Separated by 7.5% SDS-PAGE.
All lanes: Western blot - Anti-VPS35 antibody [HL1017] (ab308334) at 30 µg
All lanes: Whole zebrafish lysate at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
Predicted band size: 91.7 kDa
Western blot - Anti-VPS35 antibody [HL1017] (ab308334)
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (ab308334) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-VPS35 antibody [HL1017] (ab308334) at 1/1000 dilution
Lane 1: HEK293T cell lysate at 30 µg
Lane 2: A431 cell lysate at 30 µg
Lane 3: HeLa cell lysate at 30 µg
Lane 4: HepG2 cell lysate at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 91.7 kDa
Immunohistochemistry paraffin embedded sections - Anti-VPS35 antibody [HL1017] (ab308334)
Immunohistochemical analysis of rat testis tissue labeling VPS35 using ab308334 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min
Immunohistochemistry paraffin embedded sections - Anti-VPS35 antibody [HL1017] (ab308334)
Immunohistochemical analysis of mouse testis and pancreas tissue labeling VPS35 using ab308334 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/ Immunofluorescence - Anti-VPS35 antibody [HL1017] (ab308334)
For immunofluorescence analysis, HeLa cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of VPS35 using ab308334 at 1/500 dilution (green).
Immunohistochemistry paraffin embedded sections - Anti-VPS35 antibody [HL1017] (ab308334)
Immunohistochemical analysis of human lung cancer tissue labeling VPS35 using ab308334 at 1/50 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min