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AB247016

Anti-VPS36 antibody

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(2 Publications)

Rabbit Polyclonal VPS36 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VPS36 aa 50-150.

View Alternative Names

C13orf9, EAP45, CGI-145, VPS36, Vacuolar protein-sorting-associated protein 36, ELL-associated protein of 45 kDa, ESCRT-II complex subunit VPS36

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-VPS36 antibody (AB247016)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-VPS36 antibody (AB247016)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling VPS36 using ab247016 at 4 μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS36 antibody (AB247016)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS36 antibody (AB247016)

Paraffin-embedded human testis tissue stained for VPS36 with ab247016 at a 1/20 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human VPS36 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q86VN1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Binds phosphoinosides such as PtdIns(3,4,5)P3.
See full target information VPS36
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 221: PubMed39545935

2024

MCRS1 sensitizes T cell-dependent immunotherapy by augmenting MHC-I expression in solid tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Li,Han Yi,Zheyu Jin,Kaitao Jiang,Kangkang Xue,Jin Wang,Yuping Qian,Qian Xiang,Sijing Zhu,Runhe Yan,Yulong Yang,Shenfei Sun,Kai Li,Zichu Zhou,Wei Yu,Ning Jiang,Chen Ding,Xinhua Lin,Jiang Zhong,Yuchao Dong,Yanfang Liu,Xiaofei Yu

Science (New York, N.Y.) 374:eabk0410 PubMed34882480

2021

PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation.

Applications

Unspecified application

Species

Unspecified reactive species

Federico Gulluni,Lorenzo Prever,Huayi Li,Petra Krafcikova,Ilaria Corrado,Wen-Ting Lo,Jean Piero Margaria,Anlu Chen,Maria Chiara De Santis,Sophie J Cnudde,Joseph Fogerty,Alex Yuan,Alberto Massarotti,Nasrin Torabi Sarijalo,Oscar Vadas,Roger L Williams,Marcus Thelen,David R Powell,Markus Schueler,Michael S Wiesener,Tamas Balla,Hagit N Baris,Dov Tiosano,Brian M McDermott,Brian D Perkins,Alessandra Ghigo,Miriam Martini,Volker Haucke,Evzen Boura,Giorgio Roberto Merlo,David A Buchner,Emilio Hirsch
View all publications
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Product promise

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