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AB102687

Anti-VPS4B/MIG1 antibody

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(4 Publications)

Rabbit Polyclonal VPS4B/MIG1 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VPS4B aa 150 to C-terminus.

View Alternative Names

SKD1, VPS42, MIG1, VPS4B, Vacuolar protein sorting-associated protein 4B, Cell migration-inducing gene 1 protein, Suppressor of K(+) transport growth defect 1, Protein SKD1

1 Images
Western blot - Anti-VPS4B/MIG1 antibody (AB102687)
  • WB

Supplier Data

Western blot - Anti-VPS4B/MIG1 antibody (AB102687)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-VPS4B/MIG1 antibody (ab102687) at 1/3000 dilution

Lane 1:

HaCaT whole cell extracts at 30 µg

Lane 2:

293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human VPS4B aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75351

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their ATP-dependent disassembly, possibly in combination with membrane fission (PubMed : 18687924). Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed : 22660413).. (Microbial infection) In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).
See full target information VPS4B
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 80:258 PubMed37594630

2023

Protease-independent control of parthanatos by HtrA2/Omi.

Applications

Unspecified application

Species

Unspecified reactive species

Jonas Weiß,Michelle Heib,Thiemo Korn,Justus Hoyer,Johaiber Fuchslocher Chico,Susann Voigt,Tomas Koudelka,Andreas Tholey,Dieter Adam

Nature cell biology 22:187-199 PubMed31932738

2020

The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew M Leidal,Hector H Huang,Timothy Marsh,Tina Solvik,Dachuan Zhang,Jordan Ye,FuiBoon Kai,Juliet Goldsmith,Jennifer Y Liu,Yu-Hsin Huang,Teresa Monkkonen,Ariadne Vlahakis,Eric J Huang,Hani Goodarzi,Li Yu,Arun P Wiita,Jayanta Debnath

Nature communications 10:1387 PubMed30918259

2019

Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Ju Im,Chan-Hyeong Lee,Pyong-Gon Moon,Gunassekaran Gowri Rangaswamy,Byungheon Lee,Jae Man Lee,Jae-Chul Lee,Jun-Goo Jee,Jong-Sup Bae,Taeg-Kyu Kwon,Keon-Wook Kang,Myeong-Seon Jeong,Joo-Eun Lee,Hyun-Suk Jung,Hyun-Joo Ro,Sangmi Jun,Wonku Kang,Seung-Yong Seo,Young-Eun Cho,Byoung-Joon Song,Moon-Chang Baek

PLoS pathogens 10:e1004390 PubMed25233119

2014

Crimean-Congo hemorrhagic fever virus entry into host cells occurs through the multivesicular body and requires ESCRT regulators.

Applications

Unspecified application

Species

Human

Olena Shtanko,Raisa A Nikitina,Cengiz Z Altuntas,Alexander A Chepurnov,Robert A Davey
View all publications
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Product promise

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