AB137027

Anti-VPS4B/MIG1 antibody [EPR9225]

RabMAb
Recombinant
What is this?

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(3 Publications)

Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027) is a rabbit monoclonal antibody detecting VPS4B/MIG1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

SKD1, VPS42, MIG1, VPS4B, Vacuolar protein sorting-associated protein 4B, Cell migration-inducing gene 1 protein, Suppressor of K(+) transport growth defect 1, Protein SKD1

2 Images

Flow Cytometry (Intracellular) - Anti-VPS4B/MIG1 antibody [EPR9225] (AB137027)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-VPS4B/MIG1 antibody [EPR9225] (AB137027)

Intracellular flow cytometric analysis of permeabilized 293T cells labelling VPS4B/MIG1 with ab137027 at 1/10 dilution (red). Rabbit IgG negative control is green.

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Western blot - Anti-VPS4B/MIG1 antibody [EPR9225] (AB137027)

All lanes:

Western blot - Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 49 kDa

false

Carrier free

Anti-VPS4B/MIG1 antibody [EPR9225] - BSA and Azide free
578 PE

PE Anti-VPS4B/MIG1 antibody [EPR9225]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-VPS4B/MIG1 antibody [EPR9225]
660 APC

APC Anti-VPS4B/MIG1 antibody [EPR9225]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-VPS4B/MIG1 antibody [EPR9225]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9225

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

What is this antibody validated in?
Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Human samples.

What is the molecular weight of VPS4B/MIG1?
Anti-VPS4B/MIG1 [EPR9225] (ab137027) specifically detects a band for VPS4B/MIG1 (UniProt: O75351) at a molecular weight of 49kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR9225] also available for your convenience: ab137027, Carrier free - ab248775, APC - ab319287, PE - ab319435, Alexa Fluor® 488 - ab319569, Alexa Fluor® 647 - ab319682

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their ATP-dependent disassembly, possibly in combination with membrane fission (PubMed : 18687924). Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed : 22660413).. (Microbial infection) In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).

See full target information VPS4B

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Proteomics 22:e2200049 PubMed36037246

2022

Proteomic characteristics of plasma and blood cells in natural aging rhesus monkeys.

Applications

Unspecified application

Species

Unspecified reactive species

Na Wu,Fuchuan Xiao,Jing Zhang,Yafei Chi,Yanan Zhai,Baian Chen,Jing Lu

Cell reports 33:108493 PubMed33326793

2020

Synthetic Lethal Interaction between the ESCRT Paralog Enzymes VPS4A and VPS4B in Cancers Harboring Loss of Chromosome 18q or 16q.

Applications

Unspecified application

Species

Unspecified reactive species

Jasper E Neggers,Brenton R Paolella,Adhana Asfaw,Michael V Rothberg,Thomas A Skipper,Annan Yang,Radha L Kalekar,John M Krill-Burger,Neekesh V Dharia,Guillaume Kugener,Jérémie Kalfon,Chen Yuan,Nancy Dumont,Alfredo Gonzalez,Mai Abdusamad,Yvonne Y Li,Liam F Spurr,Westley W Wu,Adam D Durbin,Brian M Wolpin,Federica Piccioni,David E Root,Jesse S Boehm,Andrew D Cherniack,Aviad Tsherniak,Andrew L Hong,William C Hahn,Kimberly Stegmaier,Todd R Golub,Francisca Vazquez,Andrew J Aguirre

Nature cell biology 19:787-798 PubMed28604678

2017

Dynamic subunit turnover in ESCRT-III assemblies is regulated by Vps4 to mediate membrane remodelling during cytokinesis.

Applications

Species

Unspecified reactive species

Beata E Mierzwa,Nicolas Chiaruttini,Lorena Redondo-Morata,Joachim Moser von Filseck,Julia König,Jorge Larios,Ina Poser,Thomas Müller-Reichert,Simon Scheuring,Aurélien Roux,Daniel W Gerlich

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VPS4B/MIG1 antibody [EPR9225]

Flow Cytometry (Intracellular) - Anti-VPS4B/MIG1 antibody [EPR9225] (AB137027)

Western blot - Anti-VPS4B/MIG1 antibody [EPR9225] (AB137027)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (3)

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