Anti-VPS52 antibody [EPR27031-70] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-VPS52 antibody [EPR27031-70] - BSA and Azide free (AB313786)

This data was developed using ab313785, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : Lane 1 : 37 seconds, lanes 2-3 : 125 seconds, lanes 4-6 : 48 seconds.

All lanes:

Western blot - Anti-VPS52 antibody [EPR27031-70] (<a href='/en-us/products/primary-antibodies/vps52-antibody-epr27031-70-ab313785'>ab313785</a>) at 1/1000 dilution

Lane 1:

SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 4:

A20 (mouse reticum sarcoma B lymphocyte) whole cell lysate at 20 µg

Lane 5:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 6:

NR8383 (rat lung macrophage (alveolar)) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 82 kDa

false

• WB

Supplier Data

Western blot - Anti-VPS52 antibody [EPR27031-70] - BSA and Azide free (AB313786)

This data was developed using ab313785, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In lanes 1-2, the lysates were stored at -80℃ prior to Western Blotting. The band beneath the target band (80 kDa) are likely to be degradation products. In Lane 3, the lysate was freshly made and used for Western Blotting immediately to minimize protein degradation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 92 seconds

All lanes:

Western blot - Anti-VPS52 antibody [EPR27031-70] (<a href='/en-us/products/primary-antibodies/vps52-antibody-epr27031-70-ab313785'>ab313785</a>) at 1/1000 dilution

Lane 1:

THP-1 (human monocytic leukemia monocyte) transfected with scrambled siRNA control whole cell lysate at 40 µg

Lane 2:

THP-1 transfected with siRNA specifically targeti VPS52 whole cell lysate at 40 µg

Lane 3:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 82 kDa

false

Exposure time: 92s

• WB

Lab

Western blot - Anti-VPS52 antibody [EPR27031-70] - BSA and Azide free (AB313786)

This data was developed using ab313785, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as a GAPDH loading control at 1/200000 dilution.

All lanes:

Western blot - Anti-VPS52 antibody [EPR27031-70] (<a href='/en-us/products/primary-antibodies/vps52-antibody-epr27031-70-ab313785'>ab313785</a>) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HepG2 transfected with siRNA specifically targeting VPS52 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 82 kDa

Observed band size: 82 kDa

false

Exposure time: 109s

• WB

Supplier Data

Western blot - Anti-VPS52 antibody [EPR27031-70] - BSA and Azide free (AB313786)

This data was developed using ab313785, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : Lanes 1-2 : 15 seconds, lanes 3-6 : 37 seconds.

All lanes:

Western blot - Anti-VPS52 antibody [EPR27031-70] (<a href='/en-us/products/primary-antibodies/vps52-antibody-epr27031-70-ab313785'>ab313785</a>) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

Human hippocampus tissue lysate at 20 µg

Lane 3:

Mouse hippocampus tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse testis tissue lysate at 20 µg

Lane 6:

Rat hippocampus tissue lysate at 20 µg

Secondary

Lanes 1 - 6:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/2000 dilution

Lanes 1 - 6:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/100000 dilution

Observed band size: 82 kDa

false